Are Eppendorf Micro- and Multipurpose centrifuges equipped with an imbalance switch-off function?

Yes, Micro- and Multipurpose centrifuges are equipped with an imbalance detection. As soon as an imbalance is detectetd, an error message occurs on the display and the run is stopped automatically.

What are the noise levels of Eppendorf Microcentrifuges?

The noise level of Eppendorf Microcentrifuges ranges from 49 dB(A) to 58 dB(A). What does that mean? Here are some examples of every day life noises: 45 db(A) - a quiet library or soft conversation at home 50 db(A) - the hum of a refrigerator 58 db(A) - dishwasher running in the next room Eppendorf Microcentrifuges have a very moderate noise level though. Please find the exact numbers in the section technical data on the respective homepages or in the respective Operating Manuals.

How can Eppendorf Micro- and Multipurpose centrifuges be opened in case of a power failure?

Each Eppendorf centrifuge is equipped with an emergency lid release. The centrifuge lid can be opened e.g. with the support of the rotor key. Detailed description of this topic is listed in the respective Operating Manual which can be downloaded from the Eppendorf homepage.

What is the minimum selectable rotational speed of an Eppendorf Microcentrifuge?

The minimum selectable speed is 100 rpm or 1 rcf (g-force).

Can the fixed-angle rotors of Eppendorf Microcentrifuges be operated without rotor lids?

The fixed-angle rotor FA-45-18-11 can be used with or without rotor lid. With rotor lid the centrifugation is aerosol-tight and the Centrifuge 5418/5418 R is even quieter. The aerosol-tight centrifugation can only be carried out with the rotor lid. Especially when working with pathogenic microorganisms, we recommend the usage of the aerosol-tight lid. When centrifuging filter devices like spin columns or other comparable tubes, the aerosol-tight rotor lid must be used in any case. This protects the centrifuge from damage – e. g. by torn-off tube lids.

What is the FastTemp function of refrigerated Eppendorf Micro- and Multipurpose centrifuges?

The FastTemp function is a specific temperature control run which was developed to reach the set temperature as quickly as possible. This temperature control run can already be started during sample preparation by pressing the FastTemp button. This enables a centrifugation at the desired temperature directly after sample preparation without time loss. Please find more detailed information of the FastTemp function in the respective operating manuals of Eppendorf Micro- and Multipurpose centrifuges on the Eppendorf homepage.

How can Eppendorf Micro- and Multipurpose centrifuges be cleaned?

For cleaning and disinfection of Eppendorf centrifuges you will find detailed information in the respective operating manuals which can be found in the section manuals on this page. Furthermore we recommend the following papers which can be found in the section Notes&Papers on the Eppendorf homepage. - Whitepaper 014 - Maintenance Poster - Logfile for Routine Maintenance

Can I use both - rpm and g-force (rcf) - to set the speed parameter of an Eppendorf Microcentrifuge?

Yes. Each Eppendorf Microcentrifuge has the possibility to use rpm or g-force (rcf) to set the speed. It is equipped with a special button to easily change between rpm and rcf. For the centrifuges Minispin / Minispin plus a combination of buttons have to be pressed. Please refer to the devices Operating Manuals for more detailed information. Information available on the product page on eppendorf.com.

Is it possible to smooth the acceleration or braking behaviour of an Eppendorf Microcentrifuge for the centrifugation of gradients or soft pellets?

Yes. The SOFT function of Eppendorf Microcentrifuges 5425R, 5427R, 5430/R was implemented to realise smooth acceleration and braking. It can be used for the centrifugation of gradients or soft pellets. With Microcentrifuges 5420 and 5425 one could choose between accelerating ramps 1 - 9 and braking ramps 0 - 9. The smaller the ramp the smoother the acceleration / braking.

What is the advantage of the automatic lid release of Eppendorf Microcentrifuges?

The automatic lid release is a feature of Eppendorf's air cooled Microcentrifuges. It can be switched On and Off via the centrifuges operating menu. If it is activated, the centrifuge lid opens automatically once the centrifugation run is finished. This allows quicker access to the samples and avoids possible heat build-up. In addition, the open lid is a clear signal - even from a distance - that the centrifugation run has ended. This function has not been implemented in the refrigerated microcentrifuges from Eppendorf, as a set temperature can be set there which should also be maintained after the end of a centrifuge run.

What benefits do I gain from the centrifugation of my samples with the swing bucket rotor?

Swing-bucket rotors are particularly advantageous for gradient centrifugation applications as horizontal layers are formed that remain in this position at the end of centrifugation (e.g. blood/Ficoll).

Which tubes can be centrifuged in Eppendorf Microcentrifuge 5427 R?

Due to the different rotors and adapters, a wide variety of tubes can be centrifuged with the Centrifuge 5427 R: • Micro test tubes (0.5 - 5 mL) • Cryo tubes • HPLC vials • PCR strips and 0.2 mL PCR tubes • Spin columns / Filter inserts (1.5/2.0 mL) Please find detailed information in the devices operating manual (chapter technical data) on the Eppendorf homepage.

Is there a certificate on the sustainability of our Centrifuge 5427 R?

Centrifuge 5427R (green coolant) an ACT Certificate is available. Please find the Certificate on the Eppendorf homepage in section Notes & Papers.

For what applications are aerosol-tight rotors important?

Aerosol-tight rotors are always recommended or even prescribed when working with hazardous or potentially hazardous substances. These can, for example, be viruses, pathogenic bacteria, potentially infectious blood samples or radioactive sample material.

What does the Finished Time function of Eppendorf Microcentrifuges mean?

The Finished Time function counts and shows the elapsed time since the end of the centrifuge run.

What is the Short Spin function of Eppendorf Micro- and Multipurpose centrifuges?

The Short Spin function was developed to be able to perform a quick centrifuge run without having to set parameters. To start a short spin centrifugation, simply press the short key. Centrifugation will continue for as long as it is pressed. Please find detailed information on this function in the respective devices manual which can be found on their Eppendorf hompages.

What kind of cooling liquid is used for the Centrifuge 5427 R?

The cooling system operates with 75g of the "green" cooling liquid R290, also known as propane.

For which applications can I use Eppendorf Microcentrifuge 5427R?

Centrifuge 5427R allows a broad variety of applications. Up to 24 micro test tubes can be centrifuged at 25,001 xg / 48 micro test tubes at 18,213 xg. It can be used with tubes from 0.2 up to 5.0 mL. Possible applications include: - Isolation of DNA, RNA and proteins - Spin columns / Filter inserts - Aerosol-tight centrifugation of infectious samples - Pelletizing of cells (bacteria, eukaryotic cells) up to 5.0 mL - Spin down of PCR mixtures in tubes or strips

Are there any additional safety features implemented due to R290?

Like all our cooling systems, the cooling system of Centrifuge 5427R is a closed system which has no leakage of cooling liquid. For the unlikely case of leakage two additional safety measures are implemeted: All potentially sparks emitting components are shielded, before the centrifuge is ready for operation, a ventilation system takes care to exchange the air in the centrifuge. Therefore, you might notice a few more seconds prolonged initialization phase.

Do I have to take special cautions during transport, storage or installation of the Centrifuge 5427 R due to the green cooling liquid?

No special transport requirements are necessary as only 75 g propane are used. This also means that the centrifuge can be transported by air freight. The room where the centrifuge 5427 R is stored and installed needs to have a volume of minimal 10 m³. In case that more than one device is installed, the requirement stays to 10 m³. During operation leave a safety clearance of 50 cm around the device and at least 30 cm to the rear of the device.

How do I recognize aerosol-tight rotors?

Firstly, the aerosol-tight rotors can be recognized by their designation abbreviation “FA” or “AT”; secondly, Eppendorf has color-coded the aerosol-tight rotors. All aerosol-tight rotors have a red rotor lid screw, and a red ring is printed on to the rotor shaft.

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Centrifuge 5427 R - For high-throughput

Centrifuge 5427 R with hydrocarbon cooling

High-throughput microcentrifuge for up to 48 x 1.5/2 mL tubes

Woman closing microcentrifuge lid with one finger touch

Centrifuge 5427 R offers a great versatility with nine different rotor options

Microcentrifuge rotor for up to 48 x 1.5/2 mL microtubes

Microcentrifuge rotor for up to 30 x 1.5/2 mL microtubes

Microcentrifuge swing-bucket rotor for 24 x 1.5 mL tubes

Microcentrifuge rotor for 24 x 1.5/2 mL spin-colum tubes

Microcentrifuge rotor for 24 x 1.5/2 mL tubes

Microcentrifuge rotor for 5 mL Eppendorf tubes

ACT certificate for sustainability score of Eppendorf Centrifuge 5427 R

ACT certificate for sustainability score of Eppendorf Centrifuge 5427 R with rotor FA45-30-11

ACT certificate for sustainability score of Eppendorf Centrifuge 5427 R with rotor FA45-48-11

ACT certificate for sustainability score of Eppendorf Centrifuge 5427 R without rotor

General Lab Product Version

Product Information

Eppendorf Centrifuge 5427 R is a high-speed, refrigerated microcentrifuge for high-throughput research. It doubles your tube capacity compared to a standard 24-place microcentrifuge thanks to its 48-place double-row rotor, while contributing to a more sustainable lab environment with its natural refrigerant.

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Learn more about sustainability at Eppendorf - incl. how to recognize a sustainable centrifuge

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You will find additional download material at the bottom of this page

Products (1)

Sets & Bundles (2)

Services (16)

Rotors (9)

Accessories (1)

1 Products

Centrifuge 5427 R (EU-IVD)

rotary knobs, refrigerated, without rotor
ACT, 230 V/50 – 60 Hz (EU)

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Catalog no. 5429000015

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€ 6.555,95

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Product Information

Technical Data

Features

Introducing our refrigerated microcentrifuge with natural refrigerant: the Eppendorf Centrifuge 5427 R

Global warming and its consequences are one of the greatest challenges of our time. Hydrofluorocarbons, which have been used as refrigerants in cooling systems such as air-conditioners as well as laboratory centrifuges or freezers in the past, can contribute to global warming due to their chemical structure. To protect our planet for future generations, it is important to switch to much more environmentally friendly, "green" cooling agents − hydrocarbons. These natural coolants have a Global Warming Potential similar to CO₂. To support this matter in the laboratory environment, Eppendorf now offers the first centrifuge in our portfolio with a natural refrigerant to protect your samples − and the planet.
The refrigerated microcentrifuge Centrifuge 5427 R was designed with high-throughput research applications in mind. With its aerosol-tight 48-place rotor it handles even the toughest challenges in a safe and reliable manner to provide you the efficiency boost you need. With speeds of up to 25,001 x g, this microcentrifuge is fast enough for all your molecular biology applications. All aerosol-tight rotors are equipped with the Eppendorf QuickLock lids for easy rotor lid opening and closing to enable safe centrifugation of hazardous samples. Choose the Centrifuge 5427 R if a 24-place microcentrifuge doesn't meet your needs regarding tube capacity and if you don't need the extra versatility our cross-over Centrifuge 5430 R can offer you.

Natural refrigerant inside!

Centrifuge 5427 R is the first Eppendorf centrifuge with hydrocarbon cooling. Hydrocarbons (HC) are also known as green or natural coolants. The two most commonly used representatives are propane and ethane (known as R290 and R170, respectively). The GWP of HC cooling liquids are more directly equivalent to CO₂. R290 is a commonly used cooling liquid in large commercial cooling systems. Due to their superior efficiency and performance, the amount of green cooling liquids used is significantly lower than that of a similar system with classic cooling by HFCs. Due to these advantages, R290 and R170 are both on the SNAP (Significant New Alternatives Policy Program for substitution of ozone-depleting substances by EPA) list for approved coolants for usage in refrigerated
laboratory instruments such as centrifuges or even ULT freezers.

Soft-touch lid closure for ergonomic operation

No need to smash the lid or use unnecessary force − just a soft finger push and you are good to go!

Simple and safe handling

Our aerosol-tight Eppendorf QuickLock rotors close with just one quarter of a turn. This not only saves you time during everyday use, it also takes strain off your wrist.

High–speed swing-bucket rotor for Eppendorf tubes

Perform gradient centrifugation or produce compact pellets with our unique swing–bucket rotor for 24 x 1.5 mL or 2 mL tubes with speeds up to 16,050 x g. Centrifuge hazardous samples safely and effortlessly thanks to the aerosol-tight QuickLock rotor lid.

New, brighly lit LCD

We did not only add a new display to our popular Centrifuge 5427 R but also new features to make your everyday lab life easier − like the one-push short spin button, the end-of run timer and an even finer time adjustment for runs <60 seconds.

Proud owner of an Eppendorf product? Already registered?

Did you know that if you register your Eppendorf Centrifuge 5427 R with us you will get the following?
• 100 epPoints^® (for each product you register) to redeem in our rewards shop
• Additional 3 months warranty when you register a centrifuge, shaker, CO₂ incubator or cycler
• Entry in an exclusive Eppendorf prize draw (for each product you register)
• Get your personal product story to be posted on the Eppendorf website

Getting started is super easy! Either -
1. Download the Eppendorf App, scan and register OR
2. Visit our webpage, log onto your myEppendorf account and enter the product serial number
More information: www.eppendorf.link/product-registration

End-of-life

Disposal of packaging
The packaging of the Eppendorf Centrifuge 5427 R contains different materials, this can include a wooden pallet, cardboard, a plastic dust cover, and some foam parts. Recycling of materials becomes more and more important every day: Are you aware that nearly all cardboard material is recycled in Europe? Our centrifuge packaging cardboard material consists of ca. 50% recycling material. Please support the global sustainability initiative of recycling valuable raw material by also collecting the freezer cardboard packaging material and disposing of it in the appropriate collection container at your organization. In respect to the plastic dust cover made of LD-PE and the foam parts, we recommend to select a dedicated recycling partner where PE material can be recycled. We suggest you contact your local waste hauler or facility management team to understand the available recycling options for your organization.

Disposal of instrument
Our Eppendorf Centrifuge 5427 R lasts for many years, but if it needs to be replaced, we kindly ask you to fulfill local requirements for disposal of these instruments. We strongly recommend a certified local recycling partner with experience in instruments with active cooling. Keeping it “local“ reduces the impact of transportation, and the "certified" aspect is recommended due to the safe and sustainable removal and recycling of the cooling liquids if it is a refrigerated centrifuge.

Decontamination
This piece of equipment was used in a laboratory and/ or was used to handle biological samples. Please keep in mind to adequately decontaminate the equipment which needs to be disposed. Check local requirements. For more information, get in contact with your local biosafety officer and/ or waste officer. Check if your local recycling partner has special instructions and/ or documentation requirements. You may also use the Eppendorf decontamination form sheet as guidance.

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Specifications	Centrifuge 5427 R (EU-IVD)

ACT label	yes
Max. RCF	25001 × g
Max. RCF with fixed-angle rotor	25001 × g
Max. RCF with swing-bucket rotor	16049 × g
Speed	100 – 16,220 rpm (50 rpm steps)
Product type	Centrifuges
Rotors available	9
Max. capacity	48 × 1.5/2.0 mL
Timer	5 s to 9:59 h
SOFT brake function	yes
Noise level	< 55 dB(A) with rotor FA-45-48-11
Power supply	230 V, 50 – 60 Hz
Max. power consumption	495 W
Dimensions (W × D × H)	31,9 × 54,0 × 25,4 cm / 12,6 × 21,3 × 10 in
Product weight	30 kg / 66,1 lb
Height (with open lid)	56 cm / 21,9 in
Cooling	refrigerated
Remote Device Monitoring and Notifications (VisioNize^®)	VisioNize^® box enabled
Temperature range	-11 °C to +40 °C (This represents the minimum and maximum temperatures that can theoretically be reached. Please note that the actual minimum and maximum temperatures depend on the rotor and speed used.)

Downloads: Centrifuge 5427 R

Selection Guide

Centrifuge Family Overview (IVD)

Overview of all Centrifuges: Capacity and Technical Data

PDF 2.2MB

English

Brochure

Protect Your Samples – And the Planet (IVD)

Centrifuge 5427 R with hydrocarbon cooling

PDF .6MB

English

Brochure

Your Daily Companion (IVD)

Eppendorf Microcentrifuges

PDF 8.0MB

English

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Centrifuge 5427 R - For high-throughput