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Eppendorf UVette®

Product Information

Working with very low amount of samples?
The UVette is a disposable UV/Vis cuvette with a light transmission of 220 nm to 1,600 nm for spectrophotometer and photometers. As an example of Eppendorf’s expertise in the production of high quality consumables, the UVette offers superb purity, convenience and precision for photometric analysis. The unique shape of the UVette combines two optical light path lengths to offer greater flexibility.

You will find additional download material at the bottom of this page

Single Devices (8)

Accessories (6)


  • original Eppendorf plastic cuvette, individually packed, (including RNase-free, DNase-free) and protein-free
  • PCR clean, 50 – 2,000 µL, 80 pcs.
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Catalog no. 0030106300

  • reclosable box
  • Eppendorf Quality™, 50 – 2,000 µL, 200 pcs.
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Catalog no. 0030106318

  • for photometer/spectrophotometer with light path height
  • 8.5 mm
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Catalog no. 4099001009

  • for photometer/spectrophotometer with light path height
  • 10 mm
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Catalog no. 4099002005

  • for photometer/spectrophotometer with light path height
  • 15 mm
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Catalog no. 4099003001

  • for photometer/spectrophotometer
  • GeneQuant I/II
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Catalog no. 4099004008

  • for photometer/spectrophotometer with light path height
  • 20 mm
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Catalog no. 4099005004

  • 80 UVettes and 1 universal adapter for light path height 15 mm, convertible to 8.5 mm
  • 50 – 2,000 µL
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Catalog no. 4099100007

Product Information

Technical Data

Features

The Eppendorf UVette allows for measurements in the UV/Vis range with transparency from 220 nm to 1,600 nm with just a typical minimum volume of 50 µL. The UVette allows the user to choose between two optical path lengths (10 mm and 2 mm). The path length can be reduced by a factor of x5 without diluting or wasting any sample – simply by turning the UVette 90°. The UVette is suitable for fluorescence assays due to its low auto-fluorescence. The PCR-clean and protein-free individually sealed variant is ideal for accurate nucleic acid and protein quantification. The UVette is ideally matched for use with the Eppendorf BioPhotometer and Eppendorf BioSpectrometer families. The UVette can be used in other spectrophotometers with the aid of an adapter.
The UVettes are manufactured from crystal clear plastic, which is UV/Vis transparent and features translucency between 220 nm and 1,600 nm. The openly accessible side walls allow for a visual check of the liquid inside the UVette from all sides. This enables the user to detect air bubbles, for example, which may alter the test result. Furthermore, the funnel–shaped design of the cuvette base prevents a capillary effect and ensures that even the minimum volume of 50 µL constantly remains centered in the measuring area.
Two packaging sizes and purity grades are available:
• The individually packaged UVettes are PCR-clean & protein-free and are the ideal choice for valuable samples. Samples can be recovered for downstream applications without the risk of degradation or contamination. Lot specific certificates are available on the Eppendorf webpage.
• The routine pack contains UVettes with Eppendorf certified quality and is the ideal choice for routine applications where many samples need to be screened. It offers safe storage in a reclosable box and convenient access to each cuvette which makes filling easy.

For safe handling, cuvettes should be handled in combination with specific cuvette holders.

Selection guide for Eppendorf photometers

One cuvette - two path lengths


The unique shape of the UVette allows sample measurement using two different optical path lengths, which are clearly marked. A rotation of the UVette by 90° provides a choice between the 10 mm and the 2 mm optical path lengths. If the sample concentration is too high when measuring using the 10 mm light path, the UVette may simply be rotated by 90°, and the measurement can be repeated in the 2 mm light path.
On the other hand, if the sample concentration is lower than expected, one can easily switch from the 2 mm to the 10 mm light path. Thus the UVette provides great flexibility by covering measurements across a broad range of concentrations. Further information, e.g. about the chemical resistance of the UVette are available in our Knowledge Base.

Design


The UVettes are manufactured from crystal clear plastic, which is UV/Vis transparent and features translucency between 220 nm and 1,600 nm. The openly accessible side walls allow for a visual check of the liquid inside the UVette from all sides. This enables the user to detect air bubbles, for example, which may alter the test result. Furthermore, the funnel-shaped design of the cuvette base prevents a capillary effect and ensures that even the minimum volume of 50 µL constantly remains centered in the measuring area.

Two packaging sizes and purity grades are available:


• The individually packaged UVettes are PCR–clean & protein–free and are the ideal choice for valuable samples. Samples can be recovered for downstream applications without the risk of degradation or contamination. Lot specific certificates are available on the Eppendorf webpage.
• The routine pack contains UVettes with Eppendorf certified quality and is the ideal choice for routine applications where many samples need to be screened. It offers safe storage in a reclosable box and convenient access to each cuvette which makes filling easy.

Safe line-up of your cuvettes needed?


Due to their light weight and the small footprint, cuvettes tend to tip on the bench. To avoid this loss of high value samples due to these accidents, a dedicated rack for cuvettes is recommended. These racks have special square-footed wells for the cuvettes.
On top, the array of several cuvettes with individual sample content in a specific rack reduces the risk of mixing up your samples.