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Liquid Handling FAQ

Pipetting into Eppendorf Tubes

01. How can I avoid dripping of the pipette when using ethanol?‌

02. How can I pipette glycerol?‌

03. Is it okay to cut the pipet tip prior to transferring glycerol?‌

04. How can I avoid foam formation inside my pipette?‌

05. How can I pipet detergents?‌

06. Why do aerosols bear a risk of contamination?‌

07. Why does a one-button operation pipette leads to less pipette contamination?‌

08. What is most important for an accurate pipetting result?‌

09. Why do I have to calibrate a pipette?‌

10. Why do I have to adjust a pipette for high density liquids?‌

11. What is an air-cushion pipette?‌

12. What does positive displacement system mean?‌

13. What is reverse pipetting?‌

14. When should I use a positive displacement system?‌

15. I have noticed that more solution is used for reverse pipetting than for normal pipetting. What is the reason for this?‌

16. Should the pipette tip be wetted with liquid before pipetting volumes smaller than 10 µL?‌

17. What is the recommendation regarding the handling of viscous liquids?‌

18. Which pipette type can be used to pipette 40 % polyethylene glycol 8000 (PEG)?‌

19. What is the best way to pipette chloroform?‌

20. What is the best way to pipette diethyl ether?‌

21. What should be considered when pipetting methanol with air-cushion pipettes?‌

22. What is the best pipette for pipetting a 99 % solution of n-amyl alcohol?‌

23. What is the best way to dispense organic solvent?‌

24. I would like to pipette dichloromethane and n-hexanol very precisely. Can I use air-cushion pipettes for this application?‌

25. How can radioactive contamination be removed from pipettes?‌

26. I very frequently work with different chemicals. Can you tell me from which point I need to adjust my pipettes?‌

27. Is it necessary to re-grease the piston of Eppendorf pipettes after autoclaving?‌

28. Do I need to disassemble the Eppendorf Research® plus before autoclaving?‌

29. Will water vapor enter the pipette while it is being autoclaved, causing water vapor to bead up on the inside of the numerical volume setting display?‌

30. How should contaminations on the outside of pipettes be removed?‌

31. How should disinfection of a pipette be performed?‌

32. What do I have to observe regarding maintenance and cleaning of pipettes?‌

33. What is an RFID chip used for?‌

34. Under which conditions will the use of low retention tips be advantageous?‌

35. Will foam formation, which is observed during pipetting of detergent containing solutions, be reduced when low retention tips are used?‌

36. Does the self-sealing mechanism of a blocking filter work with all kinds of liquids?‌