Back and forth, and back again
Washing of pellets and subsequent discarding of supernatants requires many individual pipetting steps. The same applies to pooling of protein, peptide or virus containing supernatants for subsequent analysis. This could take forever, depending on the quantity of your samples, as step by step supernatants are removed and discarded or successively transferred into a collection vessel. To remove supernatants, you have to juggle your concentration repeatedly back and forth between not only between various reaction vessels and waste containers, but also put strain on your arm to keep moving. This can become inefficient and exhausting after a while.
Multi-aspiration with an electronic pipette – surprisingly easy and efficient
The solution: multi-aspiration instead of pipetting repeatedly back and forth. You never heard about it? Multi-aspiration is the consecutive aspiration of equal volumes of supernatants in one pipette tip. Of course, at your desired speed and volume. If you already have an electronic pipette, check out this straightforward feature. The multi-aspiration mode functions in these easy to follow steps:
- Adjust the volume and pipetting speed
- Aspirate multiple supernatants successively in one tip
- Dispense or discard