Controlling Expansion and Cardiomyogenic Differentiation

To harness the potential of human pluripotent stem cells (hPSCs), abundant provision of their differentiated progenies is required. During this webinar we present how hPSC expansion as matrix-independent aggregates in suspension culture (3D) was successfully combined with cardiomyogenic differentiation. A multi-well screening approach was scaled-up to stirred-tank bioreactors applying controlled feeding strategies (Batch and Cyclic Perfusion) followed by differentiation.
Optimized conditions in 100 mL cultivations enabled the production of 40 million cardiomyocytes (>80% purity), the majority of which displayed ventricular-like action potentials and were directly applicable for bioartificial cardiac tissue formation, a potential strategy for heart repair, drug discovery and safety pharmacology.

This webinar took place on June 9, 2015.

Key Learning Objectives:

• Learn about challenges in up-scaling hPSC suspension cultures and the latest achievements using stirred-tank bioreactors.
• Understand the role of bioreactor design for cell aggregate formation and
• Learn about tools and process parameters for controlled cultivation of stem cell cultures.
  

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