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How to Facilitate Your Whole Genome Sequencing Experiment
Лабораторная академия
Whole genome sequencing offers the most comprehensive tool for genomic studies. Yet library preparation steps, including DNA extraction, quantification, fragmentation, normalization, purification and quality control pose major labor-intensive bottle necks for the generation of such sequencing data. Usually all steps are done with the aid of a kit but still, preparing a library for whole genome sequencing takes time and is a lot of work.
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To evaluate our system, we prepared 8 libraries from 200 to 300 ng DNA from Coriell Institute female reference cell line NA12878 using Nextera DNA Flex and sequenced them in two independent experiments. The average fragment size was closely clustering around ~600 bp as recommended by Illumina. Library yields were highly reproducible and comparable to manual data. This translated to good sequencing performance data with high read diversity and even coverage across the genome and autosomes and a low coefficient of variance (CV). To read further how the epMotion can contribute to optimize your NGS experiment check our white paper.
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