The power of NGS
Scoring in discovery power, sensitivity, scalability and throughput, next-generation sequencing (NGS) truly deserves to be a "next-generation" approach. The advantages are manifold. You can study whole genomes in a single NGS experiment and identify novel variants and transcripts in short time. Target regions of interest with large portions of genes can be sequenced simultaneously across a tremendous number of samples. SNPs can be discovered in large scale. The capacity of massive parallel sequencing offers many opportunities to address a variety of biological problems.
Typical error sources in NGS
PCR is the backbone of the most NGS protocols. The applications include converting samples to sequencing-ready amplicon libraries, sample/library enrichment including quality control and quantification. PCR is used in library preparation for both whole genome and targeted sequencing approaches. In NGS experiments, the processes are complex and expensive. The sample material is often very valuable. It is obvious, what counts in NGS PCR: Successful sample processing and reliable and reproducible results based on an optimal resource management. Therefore, it is worth to avoid potential sources of error.
Do you have reliability, quality and performance of cyclers and consumables in mind? If not, you should. Here comes the “why”: PCR cycler and consumables can lead to non-specific amplifications, loss of yield and can impede reliability and reproducibility of results!