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Webinar

Stem Cell Derived Exosome Production in Stirred-Tank Bioreactors

Lab Academy

Exosomes are a population of naturally occurring mobile, membrane-limited, 30 – 100 nm in diameter, extracellular vesicles containing a large number of proteins, lipids, messenger, and micro-RNAs. It was shown that they play a role in the mediation of intercellular communication, the modulation of immune-regulatory processes, tumor metabolism, and regenerative as well as degenerative processes. In recent years, there is increasing interest in the therapeutic potential of exosomes produced by Mesenchymal Stem Cells (MSCs).

Stirred-tank bioreactors offer the possibility to tightly control and monitor the production of exosomes, as well as the scalability, to produce increasing amounts. However, the cultivation of stem cells in stirred-tank bioreactors requires profound knowledge and precise control of the process.In our talk, we will highlight the potential and benefits of stirred-tank bioreactors in the cultivation of stem cells for exosome production. We will share our knowledge on how we developed the process in our DASbox® Mini Parallel Bioreactor System and scaled-up the process to 1 L controlled by our newest parallel bioreactor control system, the SciVario® twin.


Key Learning Objectives:

  • How to cultivate stem cells in a stirred-tank bioreactor:
    Learn how to identify the right cultivation method, vessel type, vessel material, and instrument configuration for your specific needs
  • Learn how to define and optimize important parameters to improve culture results – incl. Tips & Tricks
  • Live “Ask the Expert” session – Deepen your knowledge and optimize your process


Who Should Attend:


  • Scientists new to the cultivation of stem cells in bioreactors
  • Experienced scientists looking for tips and tricks to improve their stem cell culture and EV production processes


Duration:

1 hour


Speakers:

  • Jorge Escobar, Senior Research Scientist in the Applications Lab of Eppendorf, Inc.
  • Aurélie Tacheny, Project Manager and Application Specialist for Eppendorf Application Technologies


Register now and watch recording!

Speakers