96-Wells at Once – Faster and More Reproducible Cell Assays

Lab Academy 12. September 2016

Cell Based Assays are an established standard tool in life science and performed in 96 and 384-well plates. They require multichannel pipetting to ensure quick, efficient, and reproducible handling – especially when multiple plates need to be processed. A manual 12-channel pipette, an electronic 12-channel pipette and a semi-automated 96-channel electronic pipette (epMotion 96) were compared for cell seeding, cytotoxic agent and assay reagent addition.

Cell viability was evaluated after treatment with a staurosporine dose-response curve. Profiles generated were comparable for each instrument used for cell seeding and reagent adding. Nevertheless, with a manual pipetting system, the assay reproducibility was affected as indicated by higher standard deviations.

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S/B and Z´factor calculated for CellTubes - Blue Cell Viability Assay according to the instrument used for cell seeding and reagent adding

Intra- and inter-plate CVs calculated for CellTubes- Blue Cell Visability Assay for the respective instruments used

With all assay performance parameters studied (signal-to-background ratio, Z’-factor, intra-plate and inter-plate CV), it was demonstrated that the assay robustness and reproducibility were significantly better when an electronic pipette (epMotion 96 or electronic pipette) was used.

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Among all instruments tested, the epMotion 96 appears as the fastest option since a complete cell-based assay requires only 4 minutes, that means 5 times less hands-on time than what is needed with the manual pipette.

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- to read the whole Application Note.

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96-Wells at Once – Faster and More Reproducible Cell Assays

Eppendorf Deutschland

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