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Clean and secure cell thawing

Lab Academy

Thawing cells is a daily business in cell culture and microbiology labs. Usually a water bath is used for this task but it makes sense to think about new method and cleaner options. A clean way to thaw cell lines and bottled medium is to use a thermal block on a thermal mixer. We compared both methods and show the advantages of rethinking your cell thawing.

The water bath is currently the standard for thawing cell lines. It is in most laboratories and is always heated to 37 °C. The flexibility to use many different laboratory vessels, including larger bottles, is undisputed. However, it also has some disadvantages for the user and the cells. Continuous operation of the water bath, often with the lid open, because large vessels are being heated with the medium, leads to high energy consumption. In addition, the use of chemicals to disinfect the water is controversial. However, if these are not used, there is a risk of contamination. Water splashes when inserting and removing vessels can thus contaminate other vessels. Every vessel gets wet and must be dried and disinfected before it can be used in the sterile bench. The user's hands can also get wet and must be properly dried and disinfected. Gloves should be changed after removing vessels. In addition, the cleaning of a water bath is expensive. During this time, it cannot be used. Depending on the model, the subsequent warm-up time is a few minutes to an hour or longer.

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