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Versatility Solution

Purifying peripheral blood mononuclear cells (PBMC), FACS/Flow cytometry and hybridoma technology include multiple centrifugation steps of temperature-sensitive samples in many different consumables. This requires great versatility and an advanced temperature management. In addition, PBMC isolation by Ficoll® gradient centrifugation must be carried out with a minimum of vibration during deceleration (breaking) within reasonable time. Optimized acceleration and deceleration ramps speed up high-yield PBMC isolation up to 88% (see Application Note 372). Refrigerated benchtop Centrifuge 5910 Ri in combination with swing-bucket Rotor S-4xUniversal offer great versatile and an advanced cooling performance for optimal sample protection. The new user management including run records optimally supports GxP documentation.

Key Benefits

Increased Efficiency‌

Improved Sample Safety‌

Better Ergonomics‌

Components

Centrifuge 5910 Ri

  • Super versatile multipurpose centrifuge with high capacity for broad range of consumables, refrigerated for temperature sensitive samples.
  • New swing-bucket standard-rotor S-4xUniversal and its unique universal adapters accommodate bottles, plates and conical tubes.
  • Max. capacity: 4 × 1,000 mL/4 × 5 MTP

Universal Adapter 1

  • For 5.0 mL and 15 mL conical tubes, MTP/DWP
  • Max. capacity: 17 x 5/15 mL tubes or 1 x MTP/DWP
  • Max. speed: 4,324 × g (tubes), 3,237 × g (plates)

Rotor S-4xUniversal with buckets

  • Swing-bucket rotor for broad range of high capacity applications in tubes, bottles and plates
  • Aerosol-tight caps optional available.
  • Max. speed: 4,347 × g (4,500 rpm).

Universal Adapter 3

  • For 25 mL and 50 mL conical tubes, 250 mL bottles, MTP
  • Max. capacity: 7 x 25/50 mL tubes, 1 x bottles or MTP
  • Max. speed: 4,324 × g (50 mL tubes), 2,604 × g (plates)

Recommended Consumables

Key Methods

Consumables

Advantages

Antigen concentration

  • Protein LoBind® Tubes 15 mL, PCR clean
  • Protein LoBind® Tubes 50 mL, PCR clean
  • Conical Tubes 25 mL, screw cap, Starter Pack, PCR clean
  • DWP 96x2000µL White, Protein LoBind® , PCR clean
  • Minimization of unspecific sample loss with LoBind® consumables
  • Safe sample handling due to lid tightness
  • Safe centrifugation with high g-force stability & screw Cap
Hybridoma technology
  • Conical Tubes 50 mL rack, sterile
  • Conical Tubes 15 mL rack, sterile
  • Protein LoBind® Tubes 15 mL, PCR clean
  • Protein LoBind® Tubes 50 mL, PCR clean
  • Conical Tubes 25 mL, SnapTec® , sterile
  • Reduced risk of culture contamination with high grade sterile purity grade
  • Minimization of unspecific sample loss with LoBind® consumables
FACS/Flow cytometry
  • Protein LoBind® Tubes 15 mL, PCR clean
  • Protein LoBind® Tubes 50 mL, PCR clean
  • Optional: Conical Tubes 50 mL, amber, sterile
  • Optional: Conical Tubes 25 mL, screw cap, PCR clean
  • Minimization of sample photodegradation with LoBind® consumables
  • Less degradation of photo-sensitive samples due to amber material
PBMC Isolation
  • Conical Tubes 50 mL rack, sterile
  • Conical Tubes 15 mL rack, sterile
  • Conical Tubes 25 mL, screw cap, sterile
  • Reduced risk of culture contamination with high grade sterile purity grade
  • Safe sample handling due to lid tightness
  • Safe centrifugation with high g-force stability & screw Cap

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