In principle, reverse pipetting is used when working with air cushion pipettes in space – just like on earth for liquids with high viscosity, strong detergent solvents or high vapor pressure. This means that the pipetting button is pressed down to the second stop when the liquid is aspirated and only to the first stop when it is dispensed – so that after dispensing the correct volume, residual liquid remains in the tip. There are, of course, other techniques which do only make sense on Earth, such as prewetting of an air cushion when working with high volatile liquids to prevent dripping. To ensure the best possible results independent of the pipetting technique, care should also be taken to keep the air cushion between the piston and the liquid as small as possible. In some experiments, especially when working with nonaqueous liquids, a positive displacement pipette is the better choice. This is, because using this type of pipette leads to a better pipetting result in terms of accuracy and precision than air cushion pipettes.Therefore, always consider which pipetting technique you are using for an experiment – and make sure that it is always used in the same way by all persons.
Inside the ISS, everything that is not attached, floats around and even the smallest impulse can impact its direction. For example, the pipette tips from a box commonly used in terrestrial laboratories would become airborne and float through the labatory – and even more dangerous: used pipette tips could turn into dangerous contaminated projectiles after their ejection.Although they won’t come as projectiles on Earth: Be careful with your pipette tips!
Find out more from outter space, click here.