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Regarding suitability of Eppendorf bioprocess equipment in GMP-regulated applications, please reach out to your Eppendorf sales representative.

Bioprocessing in Vaccine Development

Types of vaccines

Biopharmaceutical drugs have revolutionized the treatment of a wide range of diseases and are increasingly used in nearly all branches of medicine, including vaccinations. Vaccines contain weakened or inactive components of a pathogen that trigger an immune response within the body. This teaches the immune system to recognize and establish a rapid and effective response against that pathogen in future infections. This process is critical to protecting populations from rapidly spreading or potentially fatal diseases. Vaccines come in a wide variety of forms and use either the full pathogen, subunits of it, or the genetic information for producing the antigen of choice.

Read more about the different vaccine types‌

Read about the advantages of bioreactors for vaccine production‌

Get to know more about approaches and trends in vaccine development production‌

Bioprocess development for various vaccine types

Different production methods are required since the type of candidate antigen can vary depending on the target pathogen. Eppendorf offers upstream bioprocess solutions for the development of different types of vaccines using different cell types. Read more!

Bioreactor systems for bioprocess development for viral vector production

Viruses

Viruses are usually grown in cell lines, such as MRC-5 or Vero cells (e.g., hepatitis A or the polio vaccine). Learn more about bioprocess development for making viral vectors.

Bacteria

Bacterial pathogens are grown in bioreactors using optimized process conditions in a scalable manner. Read more about bacterial fermentation.

Recombinant proteins

Recombinant proteins can be expressed in microbial cells such as bacteria and yeast, or in mammalian cell culture. Learn more about cell culture in bioreactors.

Webinar: Stirred tank bioreactors in vaccine development

The use of cell culture in bioreactors offers a standardized, scalable, and cost-effective procedure for developing vaccines. Learn, how suspension cells were efficiently cultivated in bioreactors to produce AAV particles and COVID-19 spike-proteins in suspension culture.

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Webinar announcement: AAV production in bioreactors

Methods of vaccine development

Vaccines are critical to both human and animal health. They are becoming an ever more vital tool in the fight against disease as new diseases continue to emerge against a backdrop of increasing antimicrobial resistance. To meet growing global demands for new vaccines, it is important to be able to produce vaccines in an efficient and cost-effective manner. The use of cell culture in bioreactors offers a standardized, scalable, and cost-effective procedure for developing vaccines. Find out, how Eppendorf can support you in upstream bioprocess development and scale-up.

Process development

The purpose of upstream bioprocess development is to reduce the overall manufacturing cost of vaccine development while maximizing the quantity and quality of the desired final product. Various cultivation parameters, such as the temperature, pH , DO , shear force, and nutrient supply can affect cell growth and protein expression levels. Thus, it is essential to evaluate each of these conditions and their interactions to determine the most effective conditions for your bioprocess.
Learn more about how the Eppendorf solutions help you saving time in bioprocess development.
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Bioprocess scale-up

Bioprocess insight gained at small-scale can help to ensure a high level of success in your large-scale processes. Once the ideal conditions have been achieved, bioreactors can be used to effectively scale-up your culture from benchtop to pilot and industrial scales. Several scale-up strategies exist to ensure maximum efficiency and product quality is maintained.
Learn more about scale-up strategies and how the Eppendorf bioprocess equipment can help streamlining scale-up.
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Process intensification

Process intensification, a methodology for reducing equipment, energy consumption, and waste generation while achieving higher quality products, can make a substantial contribution to further increasing process productivity and lowering production costs. One way to optimize your bioprocessing is fine-tuning the control of your bioprocess through Process Analytic Technology (PAT) which can help to improve reproducibility, reduce batch-to-batch variation, and increase yields and product quality. Another important consideration when intensifying the rate or increasing the yield is which operation mode to use for your bioprocess. A variety of strategies including batch, fed-batch, and continuous cultures exist to help you optimize batch quality and yield.
Learn more about how to reduce batch-to-batch variability and increase yield.
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Eppendorf bioprocess solutions for vaccine development

Scalable systems‌

Single-use bioreactors‌

Cell culture in perfusion‌

Cultivation of adherent cells‌

Discover the Eppendorf bioreactor portfolio!
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How our customers use our products

Find some examples of how our customers use Eppendorf bioprocess equipment for vaccine process development.

Vaxcyte®: Scaling up cell-free expression

Read, how scientists at the University of Maryland School of Medicine and Vaxcyte® , Inc. scaled-up the cell-free expression of a vaccine candidate in Eppendorf bioreactor systems.

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Hunan Cancer Hospital: Perfusion cultivation of Vero cells

Read, how researchers at the Hunan Cancer Hospital, the Hunan Clinical Medical Research Center of Gastrointestinal Tumor, and Hefei University set up perfusion cultivation of Vero cells on microcarriers using the BioFlo® 320 bioreactor control system equipped with Cell-Lift impeller and decanter column.

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Worcester Polytechnic Institute: Vero cell culture on Fibra-Cel® Disks

Scientists at the Worcester Polytechnic Institute found bioreactors with a packed-bed made of Fibra-Cel® Disks to be an excellent choice for culturing Vero cells.

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A selection of our customers

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