Technicians and scientists interested in the generation of transgenic animals. Basic experience with eukaryotic cells and microscopy required.Ziel des Seminars:
By the end of the course, participants should be able to perform microinjection into mouse zygotes as well as transfer embryonic stem cells into mouse 8-cell embryos and blastocysts using a specific micromanipulation setup.
A – Microinjection techniques for transgenic mice:
Inhalt des Seminars:
B – Theoretical and practical microscopy training:
- DNA/RNA Microinjection into zygotes
- Embryonic stem cell transfer into blastocysts
- Piezo-assisted stem cell transfer into 8-cell stage embryos
- This part of the training will be supported by Leica Microsystems
Please contact us!
Eppendorf Vertrieb Deutschland GmbH, Frau Ursula Hennecke, Peter-Henlein-Str. 2, 50389 Wesseling, Tel.: 02232-418 159, Email: email@example.com