Are Eppendorf Microcentrifuges equipped with an imbalance switch-off function?

Yes, Microcentrifuges are equipped with an imbalance detection. As soon as an imbalance is detectetd, an error message occurs on the display and the run is stopped automatically.

For which applications can I use Eppendorf Microcentrifuge 5420?

Eppendorf Microcentrifuge 5420 allows a broad variety of applications. Up to 24 micro test tubes can be centrifuged at 21,300 xg. It can be used with tubes from 0.2 up to 2.0 mL. Possible applications include: -Isolation of DNA, RNA and proteins - Spin columns / Filter inserts - Aerosol-tight centrifugation of infectious samples - Pelletizing of cells (bacteria, eukaryotic cells) up to 2.0 mL - Spin down of PCR mixtures in tubes and strips

What kind of tubes can be centrifuged in Eppendorf Microcentrifuge 5420?

Due to the 2 different rotors and adapters, a variety of tubes can be centrifuged with the Centrifuge 5420: • Micro test tubes (0.5 - 2.0 mL) • PCR tubes and strips • Spin columns / Filter inserts (1.5/2.0 mL) You will find a detailed list of usable tubes in the centrifuges operating manual (chapter technical data) which can be downloaded from the Eppendorf homepage.

What are the noise levels of Eppendorf Microcentrifuges?

The noise level of Eppendorf Microcentrifuges ranges from 49 dB(A) to 58 dB(A). What does that mean? Here are some examples of every day life noises: 45 db(A) - a quiet library or soft conversation at home 50 db(A) - the hum of a refrigerator 58 db(A) - dishwasher running in the next room Eppendorf Microcentrifuges have a very moderate noise level though. Please find the exact numbers in the section technical data on the respective homepages or in the respective Operating Manuals.

How can Eppendorf Micro- and Multipurpose centrifuges be opened in case of a power failure?

Each Eppendorf centrifuge is equipped with an emergency lid release. The centrifuge lid can be opened e.g. with the support of the rotor key. Detailed description of this topic is listed in the respective Operating Manual which can be downloaded from the Eppendorf homepage.

What is the minimum selectable rotational speed of an Eppendorf Microcentrifuge?

The minimum selectable speed is 100 rpm or 1 rcf (g-force).

Can the fixed-angle rotors of Eppendorf Microcentrifuges be operated without rotor lids?

The fixed-angle rotor FA-45-18-11 can be used with or without rotor lid. With rotor lid the centrifugation is aerosol-tight and the Centrifuge 5418/5418 R is even quieter. The aerosol-tight centrifugation can only be carried out with the rotor lid. Especially when working with pathogenic microorganisms, we recommend the usage of the aerosol-tight lid. When centrifuging filter devices like spin columns or other comparable tubes, the aerosol-tight rotor lid must be used in any case. This protects the centrifuge from damage – e. g. by torn-off tube lids.

What is the FastTemp function of refrigerated Eppendorf Micro- and Multipurpose centrifuges?

The FastTemp function is a specific temperature control run which was developed to reach the set temperature as quickly as possible. This temperature control run can already be started during sample preparation by pressing the FastTemp button. This enables a centrifugation at the desired temperature directly after sample preparation without time loss. Please find more detailed information of the FastTemp function in the respective operating manuals of Eppendorf Micro- and Multipurpose centrifuges on the Eppendorf homepage.

How can Eppendorf Micro- and Multipurpose centrifuges be cleaned?

For cleaning and disinfection of Eppendorf centrifuges you will find detailed information in the respective operating manuals which can be found in the section manuals on this page. Furthermore we recommend the following papers which can be found in the section Notes&Papers on the Eppendorf homepage. - Whitepaper 014 - Maintenance Poster - Logfile for Routine Maintenance

Can I use both - rpm and g-force (rcf) - to set the speed parameter of an Eppendorf Microcentrifuge?

Yes. Each Eppendorf Microcentrifuge has the possibility to use rpm or g-force (rcf) to set the speed. It is equipped with a special button to easily change between rpm and rcf. For the centrifuges Minispin / Minispin plus a combination of buttons have to be pressed. Please refer to the devices Operating Manuals for more detailed information. Information available on the product page on eppendorf.com.

What is the advantage of the automatic lid release of Eppendorf Microcentrifuges?

The automatic lid release is a feature of Eppendorf's air cooled Microcentrifuges. It can be switched On and Off via the centrifuges operating menu. If it is activated, the centrifuge lid opens automatically once the centrifugation run is finished. This allows quicker access to the samples and avoids possible heat build-up. In addition, the open lid is a clear signal - even from a distance - that the centrifugation run has ended. This function has not been implemented in the refrigerated microcentrifuges from Eppendorf, as a set temperature can be set there which should also be maintained after the end of a centrifuge run.

Does the Eppendorf Microcentrifuge 5420 have a possibility to protect programs against overwriting?

Yes, it has. Please find details of this function in the Operating Manual of the device.

What does the Finished Time function of Eppendorf Microcentrifuges mean?

The Finished Time function counts and shows the elapsed time since the end of the centrifuge run.

How does the Timer function of Eppendorf Microcentrifuges work?

The "Timer" function is able to delay the start of a centrifuge run. This can be used for many nucleic acid purification kits where the buffer on the column should be incubated before the sample is centrifuged. Please find detailed information in the respective devices manual on the Eppendorf homepage.

What is the Short Spin function of Eppendorf Micro- and Multipurpose centrifuges?

The Short Spin function was developed to be able to perform a quick centrifuge run without having to set parameters. To start a short spin centrifugation, simply press the short key. Centrifugation will continue for as long as it is pressed. Please find detailed information on this function in the respective devices manual which can be found on their Eppendorf hompages.

How should I lubricate the seal of the FA-24x2 rotor lid of Eppendorf Microcentrifuge 5420?

Please lubricate the FA-24x2 rotor lid as described in the Operating Manual or the rotor supplementary sheet. Information available at the product page on eppendorf.com.

How do I recognize aerosol-tight rotors?

Firstly, the aerosol-tight rotors can be recognized by their designation abbreviation “FA” or “AT”; secondly, Eppendorf has color-coded the aerosol-tight rotors. All aerosol-tight rotors have a red rotor lid screw, and a red ring is printed on to the rotor shaft.

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Centrifuge 5420

Centrifuge 5420 24x2 rotor, side view, 2

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完美适配Eppendorf离心管的离心机

EppendorfCentrifuge 5420 适合用于以离心柱为基础的核酸纯化和凝胶提取工作流程。此款新型离心机十分适配Eppendorf 离心管，其占地面积小，配备24孔气密性固定角转，最大相对离心力21,300 × g。

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Centrifuge 5420

按键式, 非冷冻, 带气密性转子FA-24x2
230 V/50 – 60 Hz (CN)

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Centrifuge 5420应用广泛

Centrifuge 5420专为分子生物学常规应用场景而设计。适用范围包括DNA/RNA纯化、琼脂糖凝胶中提取DNA 以及PCR 反应体系构建。其占地面积极小，有助节省实验室台面空间，同时配备易于清洁的按键式控制面板。您可以同时离心多达 24 个 1.5 至 2.0 mL 离心管。适配器，适用于 0.4 和 0.5 mL 微量离心管以及0.6 mL BD Microtainer^® 管。适用于 4 × PCR 排管 or 32 × PCR 单管 (0.2 mL)。

Eppendorf Centrifuge 5420 助力提升效率和安全性

为保障操作人员安全，标配转子具备气溶胶防护功能，适合离心病毒、致病菌或放射性探针等有害样本，是实验室多种应用的理想选择。

是否已购买Eppendorf产品？是否完成注册？

您知道吗，完成Eppendorf Centrifuge 5420 注册即可享有以下权益？
• 每注册一件产品即可获得100epPoints^® ，可在积分商城兑换奖品
• 完成离心机、摇床、CO₂培养箱或循环仪注册可享额外3个月保修
• 每注册一件产品即可参与一次专属Eppendorf 抽奖
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注册步骤超级简单！共有两种方式：
1、下载Eppendorf 应用程序，扫描并注册；
2、访问Eppendorf官网，登录您的myEppendorf账号并输入产品序列号
更多信息请访问：www.eppendorf.link/product-registration

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包装材料处置
Eppendorf Centrifuge 5420 的包装由不同材料组成，包括木制托架、硬纸箱、塑料防尘罩和泡沫构件。材料的回收变得越来越重要：您知道欧洲几乎回收所有的纸板材料吗？我们的离心机纸板包装含有约 50%的回收材料。请通过收集冰箱纸板包装材料并将其防止在适当的回收容器中，来支持回收有价值原材料的全球可持续发展倡议。对于由LD-PE制成的塑料防尘罩和泡沫构件，建议选择具有PE回收资质的专业合作伙伴。建议联系本地废物运输商或设施管理团队，了解适合您所在企业的回收方案。

实验仪器处置
Eppendorf Centrifuge 5420 使用寿命长达数年，如需更换，请务必遵守当地有关此类仪器处置的规定和要求。我们强烈建议您在具有主动制冷仪器方面具有丰富经验的经认证本地回收合作伙伴。坚持“本地”回收处置能够减少运输影响。处置冷冻型离心机时，为确保安全且可持续地清除并回收冷冻液，建议选择“认证”回收合作伙伴。

去污
此设备曾在实验室中使用和/或用于处理生物样本。请记住对需要处理的设备进行充分去污。查看当地要求。有关更多信息，请联系当地的生物安全官员和/或废物管理官员。检查您当地的回收合作伙伴是否有特殊说明和/或文件要求。您也可以使用Eppendorf去污表格作为指导。

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规格	5420

最大 RCF	21300 × g
最大 RCF，带固定角转	21300 × g
速度	100 – 15,060 rpm
产品类型	离心机
可用转子	2
最大容量	24 × 1.5/2.0 mL
加速时间¹⁾	15 s
减速时间¹⁾	15 s
定时器	10 秒9小时59 分钟, 可连续离心
SOFT 软刹车功能	10 ramps
噪音水平	< 56 dB(A)，含 FA-24x2 气密性固定角转
电源	230 V, 50 – 60 Hz
最大能耗	290 W
尺寸（长x宽x高）	24 × 35 × 22 cm / 9.4 × 13.8 × 8.7 in
产品重量	13 kg / 28.7 lb
开盖高度	44 cm / 17.3 in
冷冻型	非冷冻