Are Eppendorf Microcentrifuges equipped with an imbalance switch-off function?

Yes, Microcentrifuges are equipped with an imbalance detection. As soon as an imbalance is detectetd, an error message occurs on the display and the run is stopped automatically.

What are the noise levels of Eppendorf Microcentrifuges?

The noise level of Eppendorf Microcentrifuges ranges from 49 dB(A) to 58 dB(A). What does that mean? Here are some examples of every day life noises: 45 db(A) - a quiet library or soft conversation at home 50 db(A) - the hum of a refrigerator 58 db(A) - dishwasher running in the next room Eppendorf Microcentrifuges have a very moderate noise level though. Please find the exact numbers in the section technical data on the respective homepages or in the respective Operating Manuals.

How can Eppendorf Micro- and Multipurpose centrifuges be opened in case of a power failure?

Each Eppendorf centrifuge is equipped with an emergency lid release. The centrifuge lid can be opened e.g. with the support of the rotor key. Detailed description of this topic is listed in the respective Operating Manual which can be downloaded from the Eppendorf homepage.

What is the minimum selectable rotational speed of an Eppendorf Microcentrifuge?

The minimum selectable speed is 100 rpm or 1 rcf (g-force).

Can the fixed-angle rotors of Eppendorf Microcentrifuges be operated without rotor lids?

The fixed-angle rotor FA-45-18-11 can be used with or without rotor lid. With rotor lid the centrifugation is aerosol-tight and the Centrifuge 5418/5418 R is even quieter. The aerosol-tight centrifugation can only be carried out with the rotor lid. Especially when working with pathogenic microorganisms, we recommend the usage of the aerosol-tight lid. When centrifuging filter devices like spin columns or other comparable tubes, the aerosol-tight rotor lid must be used in any case. This protects the centrifuge from damage – e. g. by torn-off tube lids.

What is the FastTemp function of refrigerated Eppendorf Micro- and Multipurpose centrifuges?

The FastTemp function is a specific temperature control run which was developed to reach the set temperature as quickly as possible. This temperature control run can already be started during sample preparation by pressing the FastTemp button. This enables a centrifugation at the desired temperature directly after sample preparation without time loss. Please find more detailed information of the FastTemp function in the respective operating manuals of Eppendorf Micro- and Multipurpose centrifuges on the Eppendorf homepage.

How can Eppendorf Micro- and Multipurpose centrifuges be cleaned?

For cleaning and disinfection of Eppendorf centrifuges you will find detailed information in the respective operating manuals which can be found in the section manuals on this page. Furthermore we recommend the following papers which can be found in the section Notes&Papers on the Eppendorf homepage. - Whitepaper 014 - Maintenance Poster - Logfile for Routine Maintenance

Can I use both - rpm and g-force (rcf) - to set the speed parameter of an Eppendorf Microcentrifuge?

Yes. Each Eppendorf Microcentrifuge has the possibility to use rpm or g-force (rcf) to set the speed. It is equipped with a special button to easily change between rpm and rcf. For the centrifuges Minispin / Minispin plus a combination of buttons have to be pressed. Please refer to the devices Operating Manuals for more detailed information. Information available on the product page on eppendorf.com.

Is it possible to smooth the acceleration or braking behaviour of an Eppendorf Microcentrifuge for the centrifugation of gradients or soft pellets?

Yes. The SOFT function of Eppendorf Microcentrifuges 5425R, 5427R, 5430/R was implemented to realise smooth acceleration and braking. It can be used for the centrifugation of gradients or soft pellets. With Microcentrifuges 5420 and 5425 one could choose between accelerating ramps 1 - 9 and braking ramps 0 - 9. The smaller the ramp the smoother the acceleration / braking.

What is the advantage of the automatic lid release of Eppendorf Microcentrifuges?

The automatic lid release is a feature of Eppendorf's air cooled Microcentrifuges. It can be switched On and Off via the centrifuges operating menu. If it is activated, the centrifuge lid opens automatically once the centrifugation run is finished. This allows quicker access to the samples and avoids possible heat build-up. In addition, the open lid is a clear signal - even from a distance - that the centrifugation run has ended. This function has not been implemented in the refrigerated microcentrifuges from Eppendorf, as a set temperature can be set there which should also be maintained after the end of a centrifuge run.

What benefits do I gain from the centrifugation of my samples with the swing bucket rotor?

Swing-bucket rotors are particularly advantageous for gradient centrifugation applications as horizontal layers are formed that remain in this position at the end of centrifugation (e.g. blood/Ficoll).

Which tubes can be centrifuged in Eppendorf Microcentrifuge 5427 R?

Due to the different rotors and adapters, a wide variety of tubes can be centrifuged with the Centrifuge 5427 R: • Micro test tubes (0.5 - 5 mL) • Cryo tubes • HPLC vials • PCR strips and 0.2 mL PCR tubes • Spin columns / Filter inserts (1.5/2.0 mL) Please find detailed information in the devices operating manual (chapter technical data) on the Eppendorf homepage.

Is there a certificate on the sustainability of our Centrifuge 5427 R?

Centrifuge 5427R (green coolant) an ACT Certificate is available. Please find the Certificate on the Eppendorf homepage in section Notes & Papers.

For what applications are aerosol-tight rotors important?

Aerosol-tight rotors are always recommended or even prescribed when working with hazardous or potentially hazardous substances. These can, for example, be viruses, pathogenic bacteria, potentially infectious blood samples or radioactive sample material.

What does the Finished Time function of Eppendorf Microcentrifuges mean?

The Finished Time function counts and shows the elapsed time since the end of the centrifuge run.

What is the Short Spin function of Eppendorf Micro- and Multipurpose centrifuges?

The Short Spin function was developed to be able to perform a quick centrifuge run without having to set parameters. To start a short spin centrifugation, simply press the short key. Centrifugation will continue for as long as it is pressed. Please find detailed information on this function in the respective devices manual which can be found on their Eppendorf hompages.

What kind of cooling liquid is used for the Centrifuge 5427 R?

The cooling system operates with 75g of the "green" cooling liquid R290, also known as propane.

For which applications can I use Eppendorf Microcentrifuge 5427R?

Centrifuge 5427R allows a broad variety of applications. Up to 24 micro test tubes can be centrifuged at 25,001 xg / 48 micro test tubes at 18,213 xg. It can be used with tubes from 0.2 up to 5.0 mL. Possible applications include: - Isolation of DNA, RNA and proteins - Spin columns / Filter inserts - Aerosol-tight centrifugation of infectious samples - Pelletizing of cells (bacteria, eukaryotic cells) up to 5.0 mL - Spin down of PCR mixtures in tubes or strips

Are there any additional safety features implemented due to R290?

Like all our cooling systems, the cooling system of Centrifuge 5427R is a closed system which has no leakage of cooling liquid. For the unlikely case of leakage two additional safety measures are implemeted: All potentially sparks emitting components are shielded, before the centrifuge is ready for operation, a ventilation system takes care to exchange the air in the centrifuge. Therefore, you might notice a few more seconds prolonged initialization phase.

Do I have to take special cautions during transport, storage or installation of the Centrifuge 5427 R due to the green cooling liquid?

No special transport requirements are necessary as only 75 g propane are used. This also means that the centrifuge can be transported by air freight. The room where the centrifuge 5427 R is stored and installed needs to have a volume of minimal 10 m³. In case that more than one device is installed, the requirement stays to 10 m³. During operation leave a safety clearance of 50 cm around the device and at least 30 cm to the rear of the device.

How do I recognize aerosol-tight rotors?

Firstly, the aerosol-tight rotors can be recognized by their designation abbreviation “FA” or “AT”; secondly, Eppendorf has color-coded the aerosol-tight rotors. All aerosol-tight rotors have a red rotor lid screw, and a red ring is printed on to the rotor shaft.

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Centrifuge 5427 R

ACT certificate for sustainability score of Eppendorf Centrifuge 5427 R

ACT certificate for sustainability score of Eppendorf Centrifuge 5427 R with rotor FA45-30-11

ACT certificate for sustainability score of Eppendorf Centrifuge 5427 R with rotor FA45-48-11

ACT certificate for sustainability score of Eppendorf Centrifuge 5427 R without rotor

通用实验室产品版本(GLP)

产品信息

Eppendorf Centrifuge 5427 R 是一款用于高通量研究应用的高速冷冻微型离心机。搭载48孔双排转子，比24孔标准微型离心机的试管容量高出一倍，并且采用天然制冷剂，提高实验室工作的可持续性。

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1 产品

Centrifuge 5427 R (EU-IVD)

旋钮式, 冷冻型, 不含转子
ACT, 230 V/50 – 60 Hz (CN)

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目录编号 5429000097

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特征

此款冷冻型微量离心机采用天然制冷剂：Eppendorf Centrifuge 5427 R

全球变暖及其连锁反应是我们这个时代面临的最严峻挑战之一。在过去，氢氟烃曾在空调等冷却系统以及实验室离心机或冰箱中用作制冷剂，但由于其化学结构，可能会导致全球变暖。要为子孙后代守护好地球家园，我们必须改用更加环保的碳氢化合物类制冷剂。碳氢化合物属于天然制冷剂，其全球增温潜势与CO₂相当。为在实验室环境中践行环保理念，Eppendorf 现已推出首款采用天然制冷剂的离心机，旨在保护您的样本和我们共同的地球。
冷冻型微量离心机Centrifuge 5427 R专为高通量研究应用而设计。配备48孔气密性转子，能够安全、可靠地离心高难度样本，从而提高离心效率，满足离心需求。这款小型离心机的相对离心力高达25,001 x g，足以满足所有分子生物学应用需求。所有气密性转子均配备便于轻松打开和关闭的Eppendorf QuickLock 快速锁定转子盖，确保安全离心有害样本。如果24孔微量离心机无法满足您对离心管通量的需求，并且您不需要Centrifuge 5427 R多功能交叉型离心机所提供的额外灵活性，请选择该款Centrifuge 5430 R离心机。

内有天然制冷剂！

Centrifuge 5427 R是第一个采用碳氢化合物制冷的Eppendorf离心机。碳氢化合物（HC）也称为绿色冷却剂或天然冷却剂。两种最常见的代表性烃类气体为丙烷和乙烷（也分别被称为 R290 和 R170）。 HC冷却液的GWP更直接地等同于 CO₂。 R290是大型商用冷却系统中常用的冷却液。由于其优越的效率和性能，所使用的绿色冷却液的量明显低于使用传统HFC冷却的类似系统。由于这些优点，R290 和R170都在SNAP（美国环保署替代臭氧消耗物质的重大新替代品政策计划）清单上，以供冷藏
实验室仪器使用，如离心机或超低温冰箱。

轻触式盖子闭合，符合人体工程学操作

无需用力拍打盖子或使用不必要的力度 − 只要手指轻轻一按，您就可以离开！

操作简单安全

我们的气密性Eppendorf QuickLock转子只需转动 1/4 圈便可关闭。这不仅节省了您的日常工作时间，还可以减轻腕部压力。

–用于Eppendorf离心管的高速水平转子

使用我们独特的水平转子进行梯度离心或生产紧凑颗粒，适配 24 x 1.5 mL 或 2 mL 离心管，速度高达16,050 x g。搭载气密性QuickLock转子盖，离心危险样品时更快捷安全。

全新明亮的LCD液晶显示屏

我们不仅为广受欢迎的Centrifuge 5427 R冷冻离心机配备了全新显示屏，还添加了一些新功能，让您的日常实验室工作更加轻松——例如一键瞬时离心按钮、运行结束后的计时器以及更精确的<60秒运行时间调节。

是否已购买Eppendorf产品？是否完成注册？

您知道吗，完成EppendorfEppendorf Centrifuge 5427 R 注册即可享有以下权益？
• 每注册一件产品即可获得100epPoints^® ，可在积分商城兑换奖品
• 完成离心机、摇床、CO₂培养箱或循环仪注册可享额外3个月保修
• 每注册一件产品即可参与一次专属Eppendorf 抽奖
• 专属产品故事刊登在Eppendorf官网

注册步骤超级简单！共有两种方式：
1、下载Eppendorf 应用程序，扫描并注册；
2、访问Eppendorf官网，登录您的myEppendorf账号并输入产品序列号
更多信息请访问：www.eppendorf.link/product-registration

生命周期结束

包装材料处置
Eppendorf Centrifuge 5427 R的包装由不同材料组成，包括木制托架、硬纸箱、塑料防尘罩和泡沫构件。材料的回收变得越来越重要：您知道欧洲几乎回收所有的纸板材料吗？我们的离心机纸板包装含有约 50%的回收材料。请通过收集冰箱纸板包装材料并将其防止在适当的回收容器中，来支持回收有价值原材料的全球可持续发展倡议。对于LD-PE和泡沫制成的塑料防尘罩，我们建议选择可以回收PE材料的专门回收合作伙伴料。建议联系本地废物运输商或设施管理团队，了解适合您所在企业的回收方案。

实验仪器处置
Eppendorf Centrifuge 5427 R使用寿命长达数年，如需更换，请务必遵守当地有关此类仪器处置的规定和要求。我们强烈建议您在具有主动制冷仪器方面具有丰富经验的经认证本地回收合作伙伴。坚持“本地”回收处置能够减少运输影响。处置冷冻型离心机时，为确保安全且可持续地清除并回收冷冻液，建议选择“认证”回收合作伙伴。

去污
此设备曾在实验室中使用和/或用于处理生物样本。请记住对需要处理的设备进行充分去污。查看当地要求。有关更多信息，请联系当地的生物安全官员和/或废物管理官员。检查您当地的回收合作伙伴是否有特殊说明和/或文件要求。您也可以使用Eppendorf去污表格作为指导。

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规格	5427 R

ACT 标签	是
最大 RCF	25001 × g
最大 RCF，带固定角转	25001 × g
最大 RCF，带水平转子	16049 × g
速度	100 – 16,220 rpm (50 rpm 步进)
产品类型	离心机
可用转子	9
最大容量	48 × 1.5/2.0 mL
定时器	5 秒至9小时59 分钟
SOFT 软刹车功能	是
噪音水平	< 55 dB(A)，配有转子 FA-45-48-11
电源	230 V, 50 – 60 Hz
最大能耗	495 W
尺寸（长x宽x高）	31.9 × 54.0 × 25.4 cm / 12.6 × 21.3 × 10 in
产品重量	30 kg / 66.1 lb
开盖高度	56 cm / 21.9 in
冷冻型	冷冻型
远程设备监视和通知 (VisioNize^®)	VisioNize^®框已启用
温度范围	-11 °C to +40 °C (This represents the minimum and maximum temperatures that can theoretically be reached. Please note that the actual minimum and maximum temperatures depend on the rotor and speed used.)