Are Eppendorf consumables free of RNA?

All consumables certified with "PCR clean" are tested free of human DNA, DNase, RNase and PCR inhibitors. The following applies from our point of view: where no human DNA is detected, there can also be no RNA, which is considerably more unstable.

Are lot specific certificates for the purity grades available in the internet?

Yes, the lot specific purity certificates, so-called Test report, can be downloaded at the certificates homepage .

Which advantages do Eppendorf Polypropylene Microplates have over plates made from polystyrene?

Plates made from polypropylene may be used across a broad spectrum of applications, as they are more resistant to temperature variations as well as various chemicals than plates made from polystyrene. Furthermore, they are more robust, allowing them to be centrifuged at higher g-forces. In addition, biomolecules, such as nucleic acids and proteins, bind less strongly to polypropylene.

How may the Eppendorf PP Microplates be sealed?

Eppendorf offers a variety of sealing options for Microplates. Self-adhesive sealers, films and foils for heat sealing, as well as re-usable sealing mats, are available. Information regarding the compatibility of the different sealing methods is listed in the Application Note no. 239 .

Which advantages do the black Eppendorf PP Microplates offer?

Plates made from black material are especially well suited for fluorescence assays (e.g. PicoGreen ® ). The black wells prevent “cross talk” between wells and reduce background signals. The black Eppendorf PP Microplates feature the unique OptiTrack Matrix, the first technology to allow for fast and easy well identification in black plates. Due to carefully selected materials, the plates have very low autofluorescence. The superior signal-to-noise ratio contributes to the increased sensitivity of fluorescence assays (see Application Note no. 203 ).

Which advantages do the white Eppendorf PP Microplates offer?

Plates made from white material may be used for luminescence assays (chemiluminescence, scintillation proximity assays (SPA) etc.), as well as for fluorescence assays (fluorescence intensity, polarization, etc.). The white wells prevent “cross talk” between the wells while amplifying the individual signals. The white Eppendorf PP Microplates feature the unique OptiTrack Matrix, the first technology to allow for fast and easy well identification in white plates. Due to carefully selected materials, the plates offer maximum signal amplification. Due to their very low autofluorescence, the white Eppendorf Microplates are equally well suited for fluorescence assays. Taken together, these features allow for an improvement in the sensitivity of analyses.

May the Eppendorf PP Microplates be stored at –196°C in liquid nitrogen?

No, we recommend storage at a minimum of –80°C.

May the Eppendorf PP Microplates be autoclaved?

Yes; however, the exact specifications of the Microplates could be compromised. The plates are available in the qualities sterile or PCR clean. Hence, autoclaving is necessary only under exceptional circumstances.

Are the Eppendorf PP Microplates in LoBind quality coated or siliconized?

No, the LoBind quality of the Eppendorf PP Microplates is not derived from siliconization or any other type of coating. The functionality is achieved through a carefully selected material, in combination with a special manufacturing process.

How high may Eppendorf PP Microplates be centrifuged?

The Eppendorf PP Microplates may be centrifuged up to 6000 x g. Please follow the instructions provided in the manual.

May immunoassays such as ELISA be performed in the Eppendorf PP Microplates?

Plates made from polypropylene are not suited for the standard variant, where antigen or antibodies are bound to the well wall. For this application, plates made from polystyrene are used, which have a higher binding capacity for biomolecules. However, for antibody-based fluorescence or luminescence assays (e.g. with beads) Eppendorf PP Microplates with black or white wells are suitable, respectively.

Is it possible to perform absorption measurements in the Eppendorf PP Microplates?

Typically, absorption measurements are not performed in plates made from polypropylene, but in plates made from polystyrene, as this material generally has a very high transparency. Since the Eppendorf PP Microplates show considerably higher transparency compared to other plates made from polypropylene (see Application Note no. 202 ), the Eppendorf Microplates may be used for absorption measurements in certain cases.

Why are the Eppendorf PP Microplates offered in Sterile and PCR clean, but not in Eppendorf quality?

The Eppendorf PP Microplates are a very high quality product. In order to underline the quality, all plates are available in the degrees of purity PCR clean or Sterile. This increases the confidence and comfort of the user, who may employ the plates directly for valuable samples in sophisticated applications.

Are Eppendorf PP Microplates also available with barcode?

Yes, the option of Eppendorf Microplates with barcode is available. Further information can be found on the product page microplates .

Are Eppendorf tubes, plates (also LoBind variants), and pipette tips resistant to 100% acetonitrile?

Eppendorf tubes, plates including LoBind, and pipette tips are resistant to 100% acetonitrile only in room temperature conditions. Above 40°C and prolonged exposure (days) may lead to partial damage and is not recommended. For lower acetonitrile concentrations the resistance parameters will be better so prolonged exposure and higher temperatures may be possible. This must be however tested by the customer.

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Eppendorf Microplates & Assay/Reader Plates

Stacked assay reader plates - clear, white and black

产品信息

Eppendorf Microplates 微孔板与Assay/Reader Plates检测分析板具备卓越的透光度，可显著提升微孔板检测灵敏度与可复现性。采用高品质聚丙烯制成，单孔性能经过优化，易于把控整个实验流程，实现样本高效回收。且信噪比高、检出限低，让每一次检测结果都值得信赖。无论是开展荧光检测、化学发光检测还是可见光检测，总有一款微孔板能够满足各项检测需求。

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产品信息

用途

特征

Eppendorf Microplates微孔板助力简化移液、管理和样本回收操作。OptiTrack^® matrix标签采用字母数字组合，易于读取，助您快速定位单个孔井，简化操作流程，降低移液操作失误风险。Eppendorf Microplates微孔板由高透光聚丙烯制成，一眼即可轻松找到样本。如需尽可能提高样本回收率，请选购LoBind^®低吸附微孔板。Assay/Reader plates系列专为可靠测量而设计，无论是可见光吸光度检测、荧光检测还是化学发光检测，均能适配顶读式酶标仪。
即使是低浓度样本，也能产生清晰信号。黑色微孔板信噪比高、检出限低，适合用于荧光检测。发光检测或弱荧光检测则推荐选用白色microplates微孔板，其表面能够最大限度反射光线，从而提升检测灵敏度。

优越透光性与OptiTrack^® matrix标识系统

Eppendorf Microplates微孔板采用优质聚丙烯制成，兼具优越透光性及可复现性，敬请放心使用。高对比度OptiTrack matrix标识系统有助快速识别孔井、降低出错和污染风险，从而提高实验室工作效率。
了解关于Eppendorf Microplates微孔板如何用于快速样本鉴定的更多信息，请参阅第202期应用说明《Eppendorf聚丙烯微孔板：快速准确识别样本》。

采用RecoverMax^®孔井设计，可在更短时间内实现更高回收率

Eppendorf微孔板采用RecoverMax^®优化孔井结构，确保达到出色的样本回收率和混匀性能。孔角圆滑，有助防止样本损失并尽可能减少残留；方形板架轮廓流畅规整，能够切实杜绝交叉污染风险。

Eppendorf Microplates微孔板透明度高、品质卓越，现已推出多种规格：

• 96孔与384孔
• 平底（F）、圆底（U）和锥底（V）
• 纯度等级包括Eppendorf PCR clean洁净级和Sterile无菌级
• 同步推出DNA和Protein LoBind低吸附型号
• g–Safe^®：可承受高达6,000 x g离心力，具有优秀的离心稳定性，有助于加快实验流程并提升样本质量。
• 孔缘加高、孔井表面光滑，确保可靠密封，即使进行热封也无影响。
• 高品质聚丙烯材质，确保化学耐受性、耐机械应力和耐极端温度。
• 可高温高压灭菌（121 °C、20 分钟）。
• 生产过程中不使用脱模剂、塑化剂和抑菌剂。

更多信息请参阅第56期应用说明《原装Eppendorf离心管 ^®、吸头与工作板的高品质材料：聚丙烯特性及耐化学性》，以及第239期应用说明《Eppendorf深孔板和微孔板密封方法研究》。

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Quality – Stability – Purity: Eppendorf Plates^®

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Eppendorf Microplates & Assay/Reader Plates

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产品信息

优越透光性与OptiTrack® matrix标识系统

采用RecoverMax®孔井设计，可在更短时间内实现更高回收率

Eppendorf Microplates微孔板透明度高、品质卓越，现已推出多种规格：

用途

特征

下载: Eppendorf Microplates & Assay/Reader Plates

优越透光性与OptiTrack^® matrix标识系统

采用RecoverMax^®孔井设计，可在更短时间内实现更高回收率