Are Eppendorf Microcentrifuges equipped with an imbalance switch-off function?

Yes, Microcentrifuges are equipped with an imbalance detection. As soon as an imbalance is detectetd, an error message occurs on the display and the run is stopped automatically.

For which applications can I use Eppendorf Microcentrifuge 5425/5425R?

Centrifuge 5425/5425R allows a broad variety of applications. Up to 24 micro test tubes can be centrifuged at 21,300 xg. It can be used with tubes from 0.2 up to 5.0 mL. Possible applications include: - Isolation of DNA, RNA and proteins - Spin columns / Filter inserts - Aerosol-tight centrifugation of infectious samples - Pelletizing of cells (bacteria, eukaryotic cells) up to 5.0 mL - Spin down of PCR mixtures in tubes or strips (12 x 8 strips or 1/4 PCR plate divisable)

What kind of tubes can be centrifuged in Eppendorf Microcentrifuge 5425/5425R?

Due to the 6 different rotors and adapters, a variety of tubes can be centrifuged with the Centrifuge 5425/5425R: • Micro test tubes (0.5 - 5.0 mL) • PCR tubes and strips and 1/4 divisable PCR plates • Spin columns / Filter inserts (1.5/2.0 mL) • Cryo tubes • HPLC tubes You will find a detailed list of usable tubes in the centrifuges Operating Manual (chapter technical data) which can be downloaded from the homepage.

What are the noise levels of Eppendorf Microcentrifuges?

The noise level of Eppendorf Microcentrifuges ranges from 49 dB(A) to 58 dB(A). What does that mean? Here are some examples of every day life noises: 45 db(A) - a quiet library or soft conversation at home 50 db(A) - the hum of a refrigerator 58 db(A) - dishwasher running in the next room Eppendorf Microcentrifuges have a very moderate noise level though. Please find the exact numbers in the section technical data on the respective homepages or in the respective Operating Manuals.

How can Eppendorf Micro- and Multipurpose centrifuges be opened in case of a power failure?

Each Eppendorf centrifuge is equipped with an emergency lid release. The centrifuge lid can be opened e.g. with the support of the rotor key. Detailed description of this topic is listed in the respective Operating Manual which can be downloaded from the Eppendorf homepage.

What is the minimum selectable rotational speed of an Eppendorf Microcentrifuge?

The minimum selectable speed is 100 rpm or 1 rcf (g-force).

Can the fixed-angle rotors of Eppendorf Microcentrifuges be operated without rotor lids?

The fixed-angle rotor FA-45-18-11 can be used with or without rotor lid. With rotor lid the centrifugation is aerosol-tight and the Centrifuge 5418/5418 R is even quieter. The aerosol-tight centrifugation can only be carried out with the rotor lid. Especially when working with pathogenic microorganisms, we recommend the usage of the aerosol-tight lid. When centrifuging filter devices like spin columns or other comparable tubes, the aerosol-tight rotor lid must be used in any case. This protects the centrifuge from damage – e. g. by torn-off tube lids.

What is the FastTemp function of refrigerated Eppendorf Micro- and Multipurpose centrifuges?

The FastTemp function is a specific temperature control run which was developed to reach the set temperature as quickly as possible. This temperature control run can already be started during sample preparation by pressing the FastTemp button. This enables a centrifugation at the desired temperature directly after sample preparation without time loss. Please find more detailed information of the FastTemp function in the respective operating manuals of Eppendorf Micro- and Multipurpose centrifuges on the Eppendorf homepage.

How can Eppendorf Micro- and Multipurpose centrifuges be cleaned?

For cleaning and disinfection of Eppendorf centrifuges you will find detailed information in the respective operating manuals which can be found in the section manuals on this page. Furthermore we recommend the following papers which can be found in the section Notes&Papers on the Eppendorf homepage. - Whitepaper 014 - Maintenance Poster - Logfile for Routine Maintenance

Can I use both - rpm and g-force (rcf) - to set the speed parameter of an Eppendorf Microcentrifuge?

Yes. Each Eppendorf Microcentrifuge has the possibility to use rpm or g-force (rcf) to set the speed. It is equipped with a special button to easily change between rpm and rcf. For the centrifuges Minispin / Minispin plus a combination of buttons have to be pressed. Please refer to the devices Operating Manuals for more detailed information. Information available on the product page on eppendorf.com.

Is it possible to smooth the acceleration or braking behaviour of an Eppendorf Microcentrifuge for the centrifugation of gradients or soft pellets?

Yes. The SOFT function of Eppendorf Microcentrifuges 5425R, 5427R, 5430/R was implemented to realise smooth acceleration and braking. It can be used for the centrifugation of gradients or soft pellets. With Microcentrifuges 5420 and 5425 one could choose between accelerating ramps 1 - 9 and braking ramps 0 - 9. The smaller the ramp the smoother the acceleration / braking.

What is the advantage of the automatic lid release of Eppendorf Microcentrifuges?

The automatic lid release is a feature of Eppendorf's air cooled Microcentrifuges. It can be switched On and Off via the centrifuges operating menu. If it is activated, the centrifuge lid opens automatically once the centrifugation run is finished. This allows quicker access to the samples and avoids possible heat build-up. In addition, the open lid is a clear signal - even from a distance - that the centrifugation run has ended. This function has not been implemented in the refrigerated microcentrifuges from Eppendorf, as a set temperature can be set there which should also be maintained after the end of a centrifuge run.

What does the Finished Time function of Eppendorf Microcentrifuges mean?

The Finished Time function counts and shows the elapsed time since the end of the centrifuge run.

How does the Timer function of Eppendorf Microcentrifuges work?

The "Timer" function is able to delay the start of a centrifuge run. This can be used for many nucleic acid purification kits where the buffer on the column should be incubated before the sample is centrifuged. Please find detailed information in the respective devices manual on the Eppendorf homepage.

What is the Short Spin function of Eppendorf Micro- and Multipurpose centrifuges?

The Short Spin function was developed to be able to perform a quick centrifuge run without having to set parameters. To start a short spin centrifugation, simply press the short key. Centrifugation will continue for as long as it is pressed. Please find detailed information on this function in the respective devices manual which can be found on their Eppendorf hompages.

How do I recognize aerosol-tight rotors?

Firstly, the aerosol-tight rotors can be recognized by their designation abbreviation “FA” or “AT”; secondly, Eppendorf has color-coded the aerosol-tight rotors. All aerosol-tight rotors have a red rotor lid screw, and a red ring is printed on to the rotor shaft.

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Centrifuge 5425/5425 R - 24位实验室标准

Centrifuge 5425 and 5425 R centrifuge Rotor FA-24 x 2-PTFE for 24 x 1.5 or 2.0 mL tubes

Eppendorf Centrifuge 5425 and 5425 R’s centrifuge Rotor FA-18 x 2-KIT

离心机5425用Eppendorf离心机转子F-32 x 0.2-PCR，带孔盖

通用实验室产品版本

IVD 产品版本

产品信息

Eppendorf Centrifuge 5425与5425 R是真正的实验室标配设备。这些 24 孔静音微型离心机适配 0.2 至 5 mL Eppendorf 离心管和PCR管，具备多项特色功能，适合一系列分子和细胞生物学应用。冷冻型Centrifuge 5425 R采用先进冷却系统，具备精准温控功能，适用于离心温度敏感型样本。

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特征

运行安静、高效

5425系列24孔微量离心机专为静音运行而设计。12 分钟以内的短时间运行非常安静，平均噪音水平为 45 分贝，最大相对离心力可达 21,300 × g。这足以完成大多数常用 DNA/RNA 纯化试剂盒的离心步骤。较长时间运行时，可以通过冷却风扇实现散热。

Eppendorf Centrifuge 5425 R与Eppendorf Centrifuge 5425 高级功能

作为热销产品Centrifuge 5424/5424 R 的新一代机型，两款新品不仅延续了前代产品的各项优点，还配备了全新转子、新增了软件功能，旨在提升日常工作效率和幸福感。

搭载改进型冷却系统Centrifuge 5425 R，可在最高转速下确保最佳分离效果，并使温度降至 4 °C。离心机可在 8 分钟内完成预冷（~21 °C 至 4 °C），可随时进入工作状态。

全系列 6 个离心机转子

这种微型离心机搭载 6 个转子，应用范围广泛。该产品首次搭载可容纳 96 根 PCR 试管的水平转子，用于在 PCR/qPCR 前后快速离心液滴和冷凝液。您还可以使用带螺旋盖或锁扣盖的固定角转，用于离心细菌和酵母（容量：10 根 5 mL 试管）。

离心期间保护管盖

Eppendorf Kit rotor F-45-18-11-Kit可在 18 根离心柱和 1.5/2.0 mL 试管中以 18565 x g 的最大速度离心样品。这种独特的转子解决了 MiniPrep 离心柱套件最常见的使用问题，即打开的离心管盖会在洗脱过程中断裂。发生这种问题的通常原因是：标准转子不能为离心过程中打开的离心管盖提供足够的支持。然而， Eppendorf Kit rotor 采用延伸边缘设计，可在离心过程中为管盖提供支撑，防止其断裂。您的管盖再也不会在离心过程中发生断裂！

用途广泛的离心机转子适配器

Eppendorf Rotor FA-10 x 5气密性固定角转可轻松实现在Eppendorf Tubes 5.0 mL 离心管中获取细菌、酵母或细胞，以及 DNA 或 RNA 和质粒DNA 的纯化。另外还提供适配器，以实现在不更换转子的情况下使用 1.5/2 mL 离心管以及 HPLC 和 Cryo 冻存管进行离心。

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是否已购买Eppendorf产品？是否完成注册？

您知道吗，完成EppendorfEppendorf Centrifuge 5425 注册即可享有以下权益？
• 每注册一件产品即可获得100epPoints^®，可在积分商城兑换奖品
• 完成离心机、摇床、CO₂培养箱或循环仪注册可享额外3个月保修
• 每注册一件产品即可参与一次专属Eppendorf 抽奖
• 专属产品故事刊登在Eppendorf官网

注册步骤超级简单！共有两种方式：
1、下载Eppendorf 应用程序，扫描并注册；
2、访问Eppendorf官网，登录您的myEppendorf账号并输入产品序列号
更多信息请访问：www.eppendorf.link/product-registration

生命周期结束

包装材料处置
Eppendorf Centrifuge 5425的包装由不同材料组成，包括木制托架、硬纸箱、塑料防尘罩和泡沫构件。材料的回收变得越来越重要：您知道欧洲几乎回收所有的纸板材料吗？我们的离心机纸板包装含有约 50%的回收材料。请通过收集冰箱纸板包装材料并将其防止在适当的回收容器中，来支持回收有价值原材料的全球可持续发展倡议。对于LD-PE和泡沫制成的塑料防尘罩，我们建议选择可以回收PE材料的专门回收合作伙伴料。建议联系本地废物运输商或设施管理团队，了解适合您所在企业的回收方案。

实验仪器处置
Eppendorf Centrifuge 5425 使用寿命长达数年，如需更换，请务必遵守当地有关此类仪器处置的规定和要求。我们强烈建议您在具有主动制冷仪器方面具有丰富经验的经认证本地回收合作伙伴。坚持“本地”回收处置能够减少运输影响。处置冷冻型离心机时，为确保安全且可持续地清除并回收冷冻液，建议选择“认证”回收合作伙伴。

去污
此设备曾在实验室中使用和/或用于处理生物样本。请记住对需要处理的设备进行充分去污。查看当地要求。有关更多信息，请联系当地的生物安全官员和/或废物管理官员。检查您当地的回收合作伙伴是否有特殊说明和/或文件要求。您也可以使用Eppendorf去污表格作为指导。

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规格	5425	5425 R

最大 RCF	21300 × g	21300 × g
最大 RCF，带固定角转	21300 × g	21300 × g
最大 RCF，带水平转子	3217 × g	3217 × g
速度	100 – 15,060 rpm	100 – 15,060 rpm
产品类型	离心机	离心机
可用转子	6	6
最大容量	10 × 5.0 mL	10 × 5.0 mL
加速时间¹⁾	15 s	15 s
减速时间¹⁾	15 s	15 s
定时器	10 秒9小时59 分钟, 可连续离心	10 秒9小时59 分钟, 可连续离心
SOFT 软刹车功能	10 ramps	是
噪音水平	前 12 分钟平均为 45 dB(A)，之后为 < 51 dB(A)，含 FA-24x2 转子	<54 dB(A)
电源	230 V, 50 – 60 Hz	230 V, 50 – 60 Hz
最大能耗	280 W	360 W
尺寸（长x宽x高）	24 × 39 × 24 cm / 9.5 × 15.4 × 9.5 in	29 × 48 × 26 cm / 11.42 × 18.9 × 10.24 in
产品重量	15.6 kg / 34.4 lb	21.0 kg / 46.3 lb
开盖高度	48 cm / 18.8 in	50.8 cm / 20.0 in
冷冻型	非冷冻	冷冻型
远程设备监视和通知 (VisioNize^®)	VisioNize^®框已启用	VisioNize^®框已启用
温度范围	−	-10 °C 至+40 °C