Webinars

Shakers and bioreactors are established versatile systems for cultivating microorganism, plant, and animal cells in suspension. Both methods aim at increasing the oxygen and nutrient supply in the medium to stimulate culture growth, but offer very different solutions in technique, volumes/throughput, control possibilities, and thus applications.

In this educational webinar, we will look at the biophysical background of both cultivation techniques, pros and cons, and areas of application. You will also learn how to optimize your process in shake flasks and bioreactors via proper choice of vessel type, vessel material, instrument configuration, and parameter settings. Additionally, we will introduce the use of feeding techniques bioreactors and have a glance on shakers too.

Date & Time

• Thursday, December 22, 2022
• 8 am PDT (Los Angeles), 11 am EDT (New York), 4 pm CET (Hamburg), 8.30 pm IST (Chennai), 11pm MYT (KL)/CST (Shanghai)
• Duration: 60 min

Target groups:

• Experienced scientists looking for tips and tricks to improve their processes
• Scientists new to cultivation of cells in suspension in shakers and/or bioreactors

Learning Objectives:

• Review: Why shake or stir cells and an outline of system specific differences
• Learn how to identify the right cultivation method, vessel type, vessel material, and instrument configuration for your specific needs
• Learn how to define and optimize important parameters to improve culture results – incl. Tips & Tricks
• Learn about feeding possibilities in shake flasks & bioreactors and their impact on your culture
• Live “Ask the Expert” session

Speaker:

• Ines Hartmann, Application Specialist Cell Handling, Eppendorf SE
   

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We often find ourselves in chaos: “Where is my key? Did I shut the fume hood? Is the PCR cycler due for maintenance again? I think I missed my bus home - let’s order an Uber.” The good news is, that nowadays, many digital tools can help us manage our private life more easily and increase our productivity in an efficient and sustainable manner. Your lab life and thus your research projects can benefit from many new digital solutions as well to save time, money and avoid headaches.

Have you ever thought about the impact of digital tools on your digital health? As new technologies emerge, they shape a more reliable way to manage data and people in the lab, however they need to be selected and implemented in a smart way. In this webinar, we will focus on the benefits of a connected digital laboratory and discuss why every lab should invest in connected digital solutions to enhance data integrity, increase efficiency and save money.

Key take aways from the webinar:

• What is digital health in the laboratory?
• How your lab benefits of investing into connected digital tools?
• How you can avoid disconnected data siloes?
• What you should consider while selecting digital tools?
• What solutions are currently available for your lab?

Covered areas in the session:

• Data Management, Data Collection, Digital Lab, Connected Lab, Data Integrity, How to ensure Digital Health, Open Ecosystems, Lab Management, Device Management, Sample Management, Avoiding Data Silos

Date & Time: 

• Tuesday, 04 Oct 2022, 3 pm CEST

Speakers:

• Zareh Zurabyan, Head of eLabNext Americas, Eppendorf Group
• Tobias Wingbermühle, CCO & Co-founder, Clustermarket
• Lynn Philip, Head of Portfolio Management, Eppendorf SE

Bio Speakers:

Zareh Zurabyan is a former biochemist researcher in the biofuels industry who has dedicated his career to improving human health by systematically assisting research and development-based labs with tech innovations to be successfully implemented in laboratories. As Head of eLabNext Americas, Zareh focuses on building a healthy Digital Strategy for labs, through Lab Digitization (ELN/LIMS), product innovation, automation, AI, and deeptech by establishing a sustainable infrastructure for laboratories. Learn more about eLabNext.

Tobias Wingbermühle is the Chief Commercial Officer and Co-founder of Clustermarket, the leading equipment scheduling and management software. He has over 8 years of sales and marketing experience in the scientific sector and has started two businesses. Learn more about clustermarket.

Lynn Philip is the head of portfolio management, digital solutions at Eppendorf SE. She is passionate about leveraging technological innovations to improve laboratory experience and to accelerate efficient scientific work. Lynn studied biochemistry at University at Buffalo and business management at University of Connecticut. She has spent 13+ years in product management in Medical Device & Life Science companies. At Eppendorf, Lynn is responsible for VisioNize portfolio of digital products that facilitates lab operations teams and scientists to make the most of their assets. Learn more about VisioNize Lab Suite.
 

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We will discuss how PCR results are influenced by multiple factors during set-up, including PCR consumables and the reaction itself.

Key learning objectives:

• Ideal PCR set-up including primer design and template DNA selection
• PCR pipetting technique
• How to minimize evaporation
• Important features of a thermal cycler
• PCR optimization

Date & Time: 

• Wednesday, 05 Oct 2022, 16:00 PM (CEST)
• Duration: 60 minutes

Speaker:

• Dr. Steffen Riethmueller, Application Specialist, Eppendorf SE

Bio Speaker:

Dr. Steffen Riethmüller is Application Specialist focusing on thermal cyclers and mixers at Eppendorf SE in Hamburg, Germany. He studied technical and applied biology in Bremen and wrote his master thesis at the Max Planck Institute in Magdeburg. During his PhD at the Institute of Biochemistry in Kiel he characterized the soluble IL-6R (which is associated with chronic inflammatory diseases) with the help of PCR and other techniques. Before joining Eppendorf, he was working as a Postdoc at the Pharmaceutical Institute in Kiel.

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The generation of novel transgenic and genome engineered mouse models routinely require the delicate procedure of zygote or 2-cell embryo microinjections. In this webinar, transgenics senior expert and trainer Dr. Aurélie Jory (Lily) will talk about proper selection and process of microinjections - for higher cell survival and blastocyst rates.

Key learning objectives:

• Introduction to the different types of mouse embryo microinjection techniques and their different applications
• Learn how to identify high quality mouse embryos for microinjection
• Tips and tricks to minimize embryo lysis and enhance editing efficiencies
• Tips for proper equipment alignment and settings
• Demonstration of mouse 1-cell zygote and 2-cell embryo pronuclear and cytoplasmic micro-injections for high cell survival rates.

Date & Time: 

• Thursday, 13 Oct 2022, 2:00 PM (CET)
• Duration: 60 minutes

Speaker:

• Dr. Aurélie Jory (Lily) - Skill Development, Training & Workshops coordinator + Project management & strategy consultant for the Mouse Genome Engineering Facility & Animal Care and Resource Center @ BLiSC Bangalore, India

Bio Speaker:

Dr. Lily received her M.Sc. and PhD degrees from the Pasteur Institute in Paris, France.  Her research at the Pasteur Institute focused on mouse myogenesis and muscle stem cell biology in the laboratory of Professor Shahragim Tajbakhsh.  From this experience, she became an expert in mouse transgenesis and acquired all the technical skills necessary to establish transgenic & genome engineered animals. She then joined the laboratory of Professor Richard Mann at Columbia University, where in addition to working with zinc finger nuclease technology to understand transcriptional regulatory networks in Drosophila development, she also served as the laboratory manager.  Dr. Lily joined Bangalore Life Science Cluster [ BLiSC ] in November 2014 to establish & manage the Mouse Genome Engineering Facility as part of the DBT funded National Mouse Resource (NaMoR) initiative under which she also served as co-head of the Animal Care and Resource Center. In 2022; taking advantage of the unique facility setup established at ACRC & MGEF, Dr Lily is redirecting her activities to focus on establishing and coordinating a dedicated high-end skill development platform providing training and workshop programs for Indian and International scientists. Dr Lily also provides project management & strategy consulting services for the Mouse Genome Engineering Facility & Animal Care & Resource Center as well as for other facilities across India.  Dr Lily is a member of the International Society for Transgenic Technologies (ISTT), of the Indian Laboratory Animal Scientists Association (LASA) and of the Indian Society of Developmental Biologists (InSDB).

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Recombinant plasmid DNA (pDNA) is produced in bacterial cultures, mostly in Escherichia coli (E. coli) in different scales from tube culture to fermenter. Successful pDNA production with consistent high yields and quality relies on multiple factors, including the vector type, the host strain, the insert, and the methodology chosen for cultivation and downstream purification.

In this webinar, we review the distinct steps in the plasmid production workflow and give tips on optimization: from vector and strain selection, to cultivation from shake flask to fermenter up to downstream processing like harvesting and purification. The focus will be on the optimization of the cultivation in shake flasks and fermenters.

Target groups:

• Scientists and technical personnel in academic and industrial setting, as well as students who start or currently work on plasmid production and want to improve their plasmid yields in tube, shake or fermenter cultures.

Key learning objectives:

• Learn about the different application fields of plasmid production
• Get an overview on the basic steps involved in plasmid production
• Learn what to consider in plasmid vector and host strain selection
• Learn how to optimize your plasmid culture in tube and shake flask culture
• Learn about stirred-tank fermenter culture set-up and scale-up considerations
• Get advice on harvesting and purification
• Live: “Ask the Expert” session – Deepen your knowledge and optimize your plasmid culture

Date & Time: 

• Wednesday, September 28, 2022, 4:00 PM (CEST)
• Duration: 45 minutes

Speaker:

• Ines Hartmann – Application Specialist Cell Handling Eppendorf SE

Bio Speaker:

Ines Hartmann is an application specialist for cell handling and responsible for the shakers and incubators at the Eppendorf headquarter in Hamburg, Germany. She joined the company in 2008 and has worked on several topics in cell handling, liquid handling and consumables. She obtained her diploma degree in biology at the Humboldt University of Berlin, Germany and gained expertise in various laboratory techniques.

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Various approaches exist for optimizing laboratory processes through digital applications ensuring not only efficiency but also safety. When it comes to a reliable sample storage process, it is mandatory for any further downstream application that your irreplaceable samples are well-maintained and remain viable in best quality. So why not considering smart tools such as sample and device monitoring software offering you the level of security you strive for?
Eppendorf drives the development of smart lab management software solutions for laboratory and device management, such as the VisioNize^® Lab Suite, which enhance not only productivity for all routine tasks in the laboratory but also offering users peace of mind.

In our webinar Thomas will highlight the benefits of remote monitoring for sample storage and laboratory processes, followed by a live demo of our Eppendorf digital platform VisioNize^® Lab Suite.

Date & Time: 

• Tuesday, September 5, 2022, 4:00 PM (CEST)

Speaker:

• Thomas Schackel, PhD – Application Specialist Eppendorf SE

Bio Speaker:

Thomas is an application specialist at Eppendorf SE whose passion became to connect scientists with their lab and IoT lab equipment. Before he joined Eppendorf in 2021, Thomas gained his PhD in neuroscience at the Spinal Cord Injury Center in the University Hospital of Heidelberg. His work was focused on a bioengineering approach combining stem cell therapy and biomaterial implants to improve neuroregeneration after traumatic spinal cord injury. At Eppendorf, Thomas is responsible to drive lab digitization via IoT lab devices facilitating scientists and lab managers to remotely monitor and manage their lab assets.

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Pipettes and dispensers are precision instruments that need some care to always work at peak performance. Depending on your application, your liquid handling tools may become a potential source of sample contamination or incorrect pipetting results if not treated properly.

Join our webinar, receive practical tips on how to clean and maintain your pipettes and learn why a regular calibration is important for the reproducibility of your research.

You'll learn:

• Why and how to properly clean pipettes and dispensers as part of your lab routine
• Practical tips & tricks for cleaning electronic pipettes
• What pipette calibration is and why it should be performed on a regular basis

Date & Time: 

• Tuesday, February 28, 2023, 16:00 (CEST)

Speaker:

• Dr. Maren Rudolph, Application Specialist Liquid Handling, Eppendorf SE
• Dr. Nadine Mellies, Application Specialist Liquid Handling, Eppendorf SE

Bio Speaker:

Dr. Maren Rudolph
Dr. Maren Rudolph is Application Specialist with a focus on Manual Liquid Handling at Eppendorf SE in Hamburg, Germany. She holds a PhD in the field of medical microbiology focusing on the visualization of bacterial proteins during host cell infection at the Institute of Medical Microbiology, Virology and Hygiene in Hamburg, Germany. Since joining Eppendorf in February 2022, she is responsible for applications of liquid handling systems and trainings.

Dr. Nadine Mellies
Being responsible for applications of liquid handling systems and trainings, Dr. Nadine Mellies is Application Specialist focusing on Manual Liquid Handling at Eppendorf SE in Hamburg, Germany. She holds a PhD in Biology with a focus on male reproductive biology working with mouse and human stem cells at the Institute of Human Genetics Goettingen, Germany. After completing her PhD thesis, she joined Eppendorf in June 2016 with 4+ years of experience in cell culture applications.

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We presented you why and when it makes sense to automate your liquid handling – but how does this automation actually look like? Join us on an introduction to the work principles of automation and learn how pipetting robots can take over critical pipetting steps or complete workflows.

We will show you examples of the automation of several key methods including extraction protocols and NGS and demo both the automation hardware and software.

You'll learn:

• How automated pipetting and sample processing works
• Automation examples of applications like NGS
• How automation software looks like and how it is used

Date & Time: 

• Tuesday, January 31, 2023, 16:00 (CEST)

Speaker:

• Dr. Tim Schommartz, Global Marketing Manager, Eppendorf SE

Bio Speaker:

Dr. Tim Schommartz works as Global Marketing Manager for automated Liquid Handling and plates at the Eppendorf Headquarters in Hamburg, Germany. He studied Molecular Life Science in Germany and Singapore. During his studies, he obtained a broad background in different workflows, ranging from molecular biology, cell- and infection biology to immunology and biochemistry. Tim Schommartz holds a Ph.D. in Virology from the University of Hamburg, focusing on viral cross-species infection. He joined Eppendorf in 2016 as an application specialist for automated liquid handling, where he acquired a deep understanding of automating lab routines.

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Despite the growing number of new technologies and automated instruments, routine liquid handling is still a manual process in many labs. The trend towards automation is however continuously gaining importance with complex applications, such as NGS library preparation.

A manifold of automated liquid handling systems, suiting all budgets and workloads, are now available: Designed to improve consistency and efficiency by eliminating both person-to-person and day-to-day variability. But how to get started with automation? When does it make sense to automate workflows and methods, and isn’t it super complicated to implement an automated liquid handler in a lab? Join our webinar to get answers to these questions!

You'll learn:

• What are the benefits of automation
• In which cases it makes sense to automate liquid handling workflows
• How automation can support even low-throughput applications

Date & Time: 

• Tuesday, November 15, 2022, 16:00 (CEST)

Speaker:

• Dr. Jessica Wagener, Application Specialist Liquid Handling, Eppendorf SE

Bio Speaker:

Jessica Wagener is Application Specialist focusing on automated liquid handling at Eppendorf SE in Hamburg, Germany. After studying biology at the universities Marburg and Duesseldorf, she started to work towards her PhD with a focus on reproductive biology at the Medical School Essen. She joined Eppendorf in 2012 as an Application Specialist for cell handling and workflows sharing her knowledge in seminars and user workshops around the world.

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Not all liquids in the lab are as easy to pipette as samples that behave like water. Many researchers have to deal with viscous, dense, volatile or foaming and even contagious samples on a daily basis.

In this webinar, you'll learn which liquid handling tools to use and techniques to employ to increase your pipetting performance and the reproducibility of your experiments with challenging liquids.

You'll learn:

• The typical challenges encountered when working with liquids that behave different than water
• How to maintain your pipetting performance with different tools, techniques and workarounds
• Which "lab myths" you shouldn't believe when dealing with challenging liquid classes

Date & Time: 

• Tuesday, October 25, 2022, 16:00 (CEST)

Speaker:

• Dr. Nadine Mellies, Application Specialist Liquid Handling, Eppendorf SE

Bio Speaker:

Being responsible for applications of liquid handling systems and trainings, Dr. Nadine Mellies is Application Specialist focusing on Manual Liquid Handling at Eppendorf SE in Hamburg, Germany. She holds a PhD in Biology with a focus on male reproductive biology working with mouse and human stem cells at the Institute of Human Genetics Goettingen, Germany. After completing her PhD thesis, she joined Eppendorf in June 2016 with 4+ years of experience in cell culture applications.

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Many scientists all over the world already upgraded to electronic pipettes to reduce strain on their thumbs and arms in the lab or to increase the reproducibility of their experiments with motor-operated pipetting cycles.

Learn why switching to electronic pipettes can be beneficial for your applications and receive answers to typical questions regarding their use and new options with connected electronic devices.

You'll learn:

• How electronic pipettes increase the reproducibility of your experiments
• How easy it can be to operate an electronic pipette
• How advanced pipetting modes are a real benefit to the efficiency of your workflow
• Watch-outs for electronic pipette maintenance and answers to misconceptions

Date & Time: 

• Tuesday, Septermber 27, 2022, 16:00 (CEST)

Speaker:

• Dr. Maren Rudolph, Application Specialist Liquid Handling, Eppendorf SE

Bio Speaker:

Dr. Maren Rudolph is Application Specialist with a focus on Manual Liquid Handling at Eppendorf SE in Hamburg, Germany. She holds a PhD in the field of medical microbiology focusing on the visualization of bacterial proteins during host cell infection at the Institute of Medical Microbiology, Virology and Hygiene in Hamburg, Germany. Since joining Eppendorf in February 2022, she is responsible for applications of liquid handling systems and trainings.

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Piston-stroke pipettes with an air-cushion are standard workhorses in labs all around the world. Did you know though, that precise pipetting results are not only created by using premium pipettes and tips but also by always employing proper pipetting technique?

In this webinar, you will learn about different pipetting techniques (such as forward/reverse pipetting) and when to use what. We also share practical tips & tricks on how to improve your pipetting skills and increase the reproducibility of your research.

You'll learn:

• How to pipette properly with air-cushion pipettes
• How to avoid typical pitfalls and increase your pipetting performance in the lab
• Why pipettes and pipette tips need to be considered as a liquid handling system

Date & Time: 

• Wednesday, Septermber 7, 2022, 16:00 (CEST)

Speaker:

• Dr. Nadine Mellies, Application Specialist Liquid Handling, Eppendorf SE

Bio Speaker:

Being responsible for applications of liquid handling systems and trainings, Dr. Nadine Mellies is Application Specialist focusing on Manual Liquid Handling at Eppendorf SE in Hamburg, Germany. She holds a PhD in Biology with a focus on male reproductive biology working with mouse and human stem cells at the Institute of Human Genetics Goettingen, Germany. After completing her PhD thesis, she joined Eppendorf in June 2016 with 4+ years of experience in cell culture applications.

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Most researchers use common air-cushion pipettes for any experiment. These pipettes however have limitations when it comes to samples that don't behave like water. Here, positive displacement pipettes or dispensers can be of great advantage.

Choosing the right liquid handling tool is critical for optimal results and can be a deciding factor for the reproducibility of your research. Join this webinar and get to know a few simple questions that will help you to find the ideal pipetting system for your experiments.

You'll learn:

• The basics of different liquid handling tool operating principles
• Key pipetting terminology: Air-cushion, positive displacement, variable/fixed volume, single-/multi-channel, …
• Which factors to consider when choosing the perfect pipette for your lab

Date & Time: 

• Wednesday, August 24, 2022, 16:00 (CEST)

Speaker:

• Dr. Nadine Mellies, Application Specialist Liquid Handling, Eppendorf SE

Bio Speaker:

Being responsible for applications of liquid handling systems and trainings, Dr. Nadine Mellies is Application Specialist focusing on Manual Liquid Handling at Eppendorf SE in Hamburg, Germany. She holds a PhD in Biology with a focus on male reproductive biology working with mouse and human stem cells at the Institute of Human Genetics Goettingen, Germany. After completing her PhD thesis, she joined Eppendorf in June 2016 with 4+ years of experience in cell culture applications.

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2020 & 2021 have shown us how important digital solutions have become to enable collaboration and to work remotely. With less access to the lab and your colleagues, the move to digital tools is even more important.

In this On Demand webinar you will be given an introduction to where the digital lab space can become a reality for you and how to begin your digital transformation.

We will cover the areas where digitization will improve your workflow and sample safety, giving you more time to focus on what is important. Your Science!

Learning Objectives

• Understanding the potential impact of the available digital solutions on your work and the benefits of "Going Digital" in the Lab
• Considerations for starting and navigating through the transformation to a digital laboratory
• Identification of starting points for your laboratory and areas where digitization will improve your workflow and sample safety

Date & Time: 

• Thursday, June 30, 2022, 5:30pm (CEST)

Speaker:

• Stephen Dey. Head of Regional Segment Marketing, Eppendorf UK

Bio Speaker:

Science background and has been involved with digitalization project at Eppendorf for the last 5 years. He currently heads the European segment marketing team.

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We will discuss the background of speed in PCR cyclers, including technical specifications and materials. We will reveal the secret of ramp rates and go into the details of PCR optimization. Finally, we will explain the correct combination of cycler, consumable and reagent to get the maximum out of your PCR.

Your learnings:

• Technical background of PCR cyclers
• What is responsible for speed in your PCR experiment
• The ideal combination for speeding up your PCR

Date & Time: Thursday, July 7, 2022, 5:00 PM (CEST), 60 minutes

Speakers: 

• Hanae König, Global Marketing Manager PCR & ThermoMixer, Eppendorf SE
• Adam Waite, Product Manager for amplification reagents, Promega

Speaker Bio:

Hanae König, Global Marketing Manager PCR & ThermoMixer, Eppendorf SE
Hanae König studied biology, focusing on medical microbiology during her PhD. After starting with Eppendorf as an application specialist for PCR she took different positions in the company and currently enjoys coordinating the global marketing of PCR and mixer devices.

Adam Waite, Product Manager for amplification reagents, Promega
Adam Waite is the Product Manager for amplification reagents at Promega in Madison, WI. He studied biology with additional focus on biochemistry at the University of Wisconsin-Madison. Shortly after, he joined Sigma-Aldrich (now MilliporeSigma) where he first supported and then managed the life science solvent product line. He completed his MBA at the University of Wisconsin-Milwaukee before transitioning to global management of MilliporeSigma’s bulk solvents portfolio. Since joining Promega in 2019, he has overseen the global expansion of Promega’s amplification reagents and continues to seek out solutions that improve researcher quality of life.

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The laboratory of today relies on digitization at every step of the experimental workflow. Though digitization offers more information, they cannot prevent failures alone because scientists need to react quickly to prevent issues, e.g. sample loss. Even the smallest change in temperature can affect the quality of a sample, so notification and monitoring systems are needed.

In this webinar, we will discuss fundamentals of prepping your lab to go digital in a strategic way with the focal point of ensuring "Sample Safety" and "Data Integrity". We will discuss healthy habits, and implementation game plans for a reliable sample storage process post choosing your leading system (ELN/LIMS/LES) of choice.

Date & Time: Monday, May 2, 2022, 5:00 PM (CEST)

Speaker: Zareh Zurabyan, Head of eLabNext, Eppendorf Group

Speaker Bio:
Zareh Zurabyan, a former biochemist researcher in the biofuels industry, has dedicated his young career to improving human health by systematically assisting research and development-based innovations to be successfully implementations in laboratories. Currently working as the Head of eLabNext Eppendorf Group. he has been responsible for growing and maintaining a consistent business growth of 200% year-after-year since the conception of his division that has accelerated management of research data. Zareh's focus on Digital Health, through Lab Digitization, product innovation, and building sustainable infrastructure for laboratories, has facilitated the efficient, compliant, and secure development of therapeutics as well as aided the healthcare system to improve human conditions.

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Contamination is an everyday challenge in cell culture labs. Undetected or ignored contamination endanger the reliability and reproducibility of experimental data. Are ethanol and antibiotics the best measures to prevent microbial contamination? Get to know the most effective methods to detect mycoplasma and to fight against the common types of contaminants. In this educational webinar you will learn how to recognize and identify different types of contaminants in cultivated cells. We will discuss how to apply aseptic techniques and preventive hygiene and give practical tips for preventing contamination by incorporating standard procedures into your daily culture practice.

Covered areas in the session:

• Types and sources of contamination
• Aseptic techniques and preventive hygiene
• Best practices to keep your lab contamination-free

Date & Time: March, 31th, 8am PDT, 11am EDT, 4pm CET, 8.30pm IST, 11pm MYT (KL)/CST

Speaker: Dr. Jessica Wagener (Application Specialist Cell Handling)

Speaker Bio:
Dr. Jessica Wagener is Application Specialist focusing on Cell Handling at Eppendorf Headquarter in Hamburg, Germany. Entering a cell culture laboratory as a graduate student for the first time in 2006, she came to Eppendorf with 5+ years of experience in cell culture. After studying Biology at the Universities of Marburg and Düsseldorf, she investigated apoptosis mechanisms in different cell lines for her diploma thesis. Following one year as Research Associate working with primary cells she started to work towards her PhD with a focus on reproductive biology at the Medical School Essen. She joined Eppendorf in April 2012 shortly after completing her PhD in Biology.

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