Webinars

Bioreactors and shakers are used to cultivate microorganisms, plant, insect, and mammalian cells in different volumes. Upscaling of production often involves shake flasks and bioreactors. Shake flasks are used for bioreactor inoculum production and are an easy-to-use instrument for early process development like organism screening and media design. At the next process steps, highly controllable bioreactors are needed for flexible solutions, automated process control, and predictable scalability when larger quantities of cells are required. The challenge during upscaling is to keep a constant efficiency and quality during all process steps.

In this webinar, we will depict the typical applications of shakers and bioreactors in an upscaling bioprocess. We will highlight applicational differences and factors that influence cultivation and system transition. Furthermore, we will introduce to you the basic components of a bioreactor and the various process parameters that can be controlled. Finally, we will go through different process strategies to further optimize a process and increase production.

Target groups:

•  Beginners, who start using shake flasks and bioreactors for cultivation and upscaling
•  Early bioreactor users, who want to deepen their knowledge and understanding of the possibilities of process control strategies

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Scheduling conflict?

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In this webinar you will:

•  Learn about typical applications of shake flasks and stirred-tank bioreactors in bioprocess development
•  Learn about influencing factors and optimizing setscrews in shaking/stirring
•  Learn about the basic bioreactor components and the different process parameters that can be monitored and controlled, and what they tell you about your culture
•  Learn about feeding possibilities and their impact on your culture

Date & Time
December 2, 2020 at 08:00 am PDT, 11:00 am EDT, 5:00 pm CEWT

Speakers
Ines Hartmann, Application Specialist Cell Handling, Eppendorf AGDavid Solbach, Scientific Communications Manager, Eppendorf Bioprocess Center
 

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An appropriate dissolved oxygen (DO) concentration in the culture medium is a key driver for high bioprocess yields. DO is determined by the oxygen supply to the bioprocess system and the oxygen consumption of the cultured microorganism simultaneously. In high-density fermentation of aerobic bacteria and fungi, for example, a large amount of oxygen must be introduced into the medium to avoid oxygen limitation during robust microbial growth. In other applications, like the cultivation of microaerophilic bacteria for probiotics production or gut microbiome research, the DO concentration must be controlled precisely at a low level.

In this webinar, we showcase advanced strategies to control the dissolved oxygen concentration in the culture medium and to reproduce it at different scales.

Key Learning Objectives:

• Importance of bioreactors’ oxygen transfer capabilities for bioprocess scale-up
• Method for measuring the oxygen transfer rate (OTR) of a bioreactor
• Hands-on expertise on how to optimize oxygen supply in high-density fermentation processes
• Hands-on expertise on how to establish microaerobic conditions for lactic acid bacteria bioprocesses

Date & Time
Tuesday, December 08, 2020 at 9am EST| 2pm BST| 3pm CEST

Speakers
Ying Yang, Senior Research Scientist, Bioprocess, Eppendorf Inc.
 

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