For which SmartBlocks can the ThermoTop be used?

The ThermoTop is compatible with folowing SmartBlocks: SmartBlock 0.5 mL SmartBlock 1.5 mLSmartBlock 2.0 mL SmartBlock plates SmartBlock PCR 96 SmartBlock PCR 384 SmartBlock DWP 500 SmartBlock DWP 1000

What is the temperature control range of the ThermoTop?

The temperture control range of the ThermoTop corresponds to the temperature control range of the device or Smartblock with which the ThermoTop is used. Further information can be found in the operating manual.

Can cell culture plates be used in the SmartBlock plates?

Cell culture plates comply with the SBS standard can be used in the SmartBlock plates. Temper polystyrene plates up to 70°C max. Further information can be found in the operating manual.

Is it possible to put the SmartBlocks for a rapid cool down into a -20°C freezer?

The SmartBlocks can be used at ambient temperatures of 5°C – 40°C and can be cooled appropriate. Cooling down of SmartBlocks in a freezer should not be done.

Which SmartBlocks and vessels can be used with ThermoMixer F models?

The ThermoMixer F models possess a fix installed SmartBlock. ThermoMixer F0.5: installed SmartBlock 0.5 mL for 24 0.5 mL mirco test tubes ThermoMixer F1.5: installed Smartblock 1.5 mL for 24 1.5 mL micro test tubes ThermoMixer F2.0: installed SmartBlock 2.0 mL for 24 2.0 mL mirco test tubes ThermoMixer FP: installed SmartBlock plates for all common plates formats Detailed informations can be found in the operating manual.

Do the IsoRacks fit upon the SmartBlocks for microreaction tubes (0.5 mL; 1.5 mL; 2.0 mL)?

No. The IsoRacks fit only upon the Thermoblocks for the older devices Thermomixer comfort, Thermomixer compact & ThermoStat plus.

Can PCR plates be used in SmartBlock plates respectively ThermoMixer FP?

SmartBlock plates and ThermoMixer FP (with the fixed SmartBlock plates) are dedicated for using MTP’s, DWP’s and also other skirted plates which meet the SBS standard. For optimal usage of PCR plates the SmartBlock PCR 96 and PCR 384 in combination with ThermoMixer C or ThermoStat C is the proper choice. These SmartBlocks possess conical bores which surround the conical wells of PCR plates properly for a perfect temperature transition.

SmartBlock cryo thaw on ThermoMixer C - What is the ‘Thawing cells’ program?

The 'Thawing cells' program is an optimized program to thaw mammalian cells in 1.8-2.0 mL cryovials: reproducibly contamination free standardized It is a ready-to use program, in most cases no changes are required. The pre-set time is 3 minutes (can be extended up to 5 minutes), the pre-set mixing frequency is 500 rpm (can be adapted between 300 – 800 rpm), the temperature profile is fixed. The settings are based on tests with standard mammalian cell lines (CHO, HEK, S16) up to highly sensitive stem cells (hiPSCs) at tested conditions with 2.0 mL cryovials / 1.0 mL fill (1 – 5 vials in parallel). Other volumes or more vials may require an adaption of thawing time. Further information can be found in the Application Note 437 .

What is the difference between the SmartBlock cryo thaw and the SmartBlock cryo?

The 'SmartBlock cryo thaw' replaces the 'SmartBlock cryo'. Newly implemented is an automatic function for thawing cells (program 'Thawing cells') which is available in combination with the ThermoMixer C.

Is it possible to use the SmartBlock cryo thaw with the ThermoStat C?

The SmartBlock cryo thaw is still compatible with the ThermoStat C. However, the automatic thawing function 'thawing cells' is limited to the ThermoMixer C as it requires mixing.

The ThermoMixer C does not show the program ‘Thawing cells’ when inserting the SmarBlock cry thaw. Why?

The software version 3.5.0 or higher is required on the ThermoMixer C to operate the program 'Thawing cells'. How to upgrade the software is described in the operating manual.

Is it possible to use the SmartExtender with the SmartBlock cryo thaw?

The Smart Extender can be used with the SmartBlock cryo thaw, but not in parallel a 'Thawing cells' program is running. In case the user is operating the SmartExtender and intends to start the thawing procedure, the user is informed by a message with the option to stop the SmartExtender and vice versa. There is no automatic stop or re-start of the running program to not disturb the application.

SmartBlock cryo thaw - Does the mixing during the thawing program harm the cells?

No, on the contrary, a slight mixing (500 rpm combined with a small 3 mm orbit) helps to distribute the temperature in the vial evenly and reduces temperature gradients and inhomogeneous warming of the cells by preventing sedimentation. The reproducibility and suitability of the thawing method have been demonstrated by: – in‑vial temperature measurements – analysis of post‑thaw cell viability – further downstream tests (confirming pluripotency for stem cells). Further information can be found in the Application Note 437 .

Is it possible to use former SmartBlock cryo with the program ‘thawing cells’?

No, the program 'thawing cells' is limited to the SmartBlock cryo thaw.

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Eppendorf SmartBlock

Eppendorf ThermoStat C com diferentes opções de Smartblocks

Remover o Eppendorf SmartBlock pressionando a alavanca (Passo 1)

Remover o Eppendorf SmartBlock pressionando a alavanca (Passo 2)

Remover o Eppendorf SmartBlock pressionando a alavanca (Passo 3)

Eppendorf SmartBlock para recipientes de 24 x 1,5 mL

Eppendorf SmartBlock para recipientes de 24 x 2,0 mL

Eppendorf SmartBlock for 8 x 5.0 mL vessels

Eppendorf SmartBlock para recipientes de 8 x 15,0 mL

Eppendorf SmartBlock para 4 recipientes de 50,0 mL

Adaptador para tubos cônicos de 25 mL a ser utilizado no Eppendorf ThermoMixer C

Eppendorf SmartBlock para recipientes de 24 x 12 mm

Eppendorf SmartBlock for 24 x cryo vessels with several cryovials

Eppendorf SmartBlock DWP 500 with white DWP

Eppendorf SmartBlock DWP 1000 com DWP na bancada

Eppendorf SmartBlock for plates, DWP branca-azul incluída

Image – Eppendorf SmartBlock for 96-well PCR plates com placa PCR azul de 96 poços com semi-saia

Eppendorf SmartBlock for 384-well PCR plates com placa PCR vermelha

Eppendorf Transfer_Rack_1.5/2.0 mL_para transferir amostras do Eppendorf ThermoMixer C

Image – Set of Eppendorf SmartBlocks with ThermoTop

Informações do produto

Substituível em segundos.
Esses SmartBlocks controlados por sensor individualmente são a sua solução para manter temperaturas exatas e constantes. Uma ampla gama de SmartBlocks para todos os tubos de laboratórios suportam várias aplicações: para utilização com o Eppendorf ThermoMixer^® C (aquecimento/refrigeração e agitação) e ThermoStat C (aquecimento/refrigeração).

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Informações do produto

Dados técnicos

Recursos

Usando tubos de tamanhos diferentes no seu fluxo de trabalho laboratorial?
A flexibilidade nunca foi tão grande. Eppendorf oferece uma ampla gama de Eppendorf SmartBlocks para tubos de 0,2 mL a 50 mL bem como placas (MTP, DWP, e placas PCR 96 / 384) para usar com Eppendorf ThermoMixer^® C e ThermoStat C. Todos os Eppendorf SmartBlocks estão equipados com o sistema Eppendorf Eppendorf QuickRelease extraordinário que torna a substituição do bloco super rápida e fácil.
Para um ajuste e transferência de temperatura ideais, recomendamos a utilização de Eppendorf Deepwell Plates com as Eppendorf SmartBlock DWP 500 e Eppendorf SmartBlock DWP 1000 correspondentes. Observe que Eppendorf SmartBlock DWP 500 e Eppendorf SmartBlock DWP 1000 só podem ser utilizados com Eppendorf Deepwell Plates.

Nenhuma ferramenta disponível?

Os Eppendorf SmartBlocks podem ser facilmente substituídos. Somente pressionando a alavanca na frente do bloco e ele pode ser removido – em alguns segundos - não são necessárias ferramentas.

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Especificações	Eppendorf SmartBlock 0.5 mL	Eppendorf SmartBlock 1.5 mL	Eppendorf SmartBlock 2.0 mL	Eppendorf SmartBlock 5 mL	Eppendorf SmartBlock 15 mL	Eppendorf SmartBlock 50 mL	Eppendorf SmartBlock PCR 96	Eppendorf SmartBlock PCR 384	Eppendorf SmartBlock plates	Eppendorf SmartBlock DWP 500	Eppendorf SmartBlock DWP 1000	Eppendorf SmartBlock cryo descongelamento	Eppendorf SmartBlock 12 mm

ThermoTop	sim	sim	sim	−	−	−	sim	sim	sim	sim	sim	não	−
Capacidade de amostras	24 × tubos microteste (0,5 mL)	24 × tubos microteste (1,5 mL)	24 × tubos microteste (2,0 mL)	8 × 5 mL	8 tubos cônicos × 15 mL	4 × tubos cônicos 50 mL	1 placa PCR 96	1 placa PCR 384	1 × placa microteste / 1 × placa deepwell	Eppendorf Deepwell Plates 96/500 µL	Eppendorf Deepwell Plates 96/1.000 µL	24 × tubos criogênicos (1,5/2,0 mL)	24 × tubos microteste, diâmetro 12 mm
SmartExtender	sim	sim	sim	sim	sim	sim	sim	sim	sim	sim	sim	sim	sim
ThermoMixer C / ThermoStat C	sim	sim	sim	sim	sim	sim	sim	sim	sim	sim	sim	sim	sim
Temp. máx.	100 °C	100 °C	100 °C	100 °C	100 °C	100 °C	100 °C	100 °C	100 °C	100 °C	100 °C	110 °C¹⁾	110 °C¹⁾
Tampa	sim	sim	sim	−	−	−	sim	sim	sim	sim	sim	−	−
Velocidade máx.	2.000 rpm	2.000 rpm	2.000 rpm	1.000 rpm	1.000 rpm	1.000 rpm	2.000 rpm	3.000 rpm²⁾	3.000 rpm³⁾	1.600 rpm	1.600 rpm	2.000 rpm	2.000 rpm

1)Somente disponível com ThermoStat C

2)3.000 rpm (2.000 com tampa)

3)3.000 (2.000 com tampa ou uso de DWP; 1.000 para uso de DWP >80 °C)

Downloads: Eppendorf SmartBlock

Folheto CD

Eppendorf ThermoMixer^® C

Our all-in-one versatile device for your everyday mixing and tempering tasks

Eppendorf Temperature Control and Mixing Instruments

PDF 2.1MB

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Eppendorf SmartBlock

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ThermoStat C Smartblock selection

Remover o Eppendorf SmartBlock 1

Remover Eppendorf SmartBlock (Passo 2)

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