How many parameters can I control using a DASGIP or DASbox system?

DASGIP Parallel Bioreactor Systems provide comprehensive concurrent monitoring and control of process parameters. During the process run, events are logged together with up to 70 online process variables per bioreactor. In addition to the various DASGIP modules for monitoring and control, with DASware analyze users can integrate analytical devices provided by third parties. Seamless integration into the DASGIP or DASbox system even allows for closed loop solutions such as automated feeding strategies based on the current glucose concentration. Each process value can be used as a trigger enabling users to automate their process.

How many parameters can be displayed in a process overview in DASware control?

DASware control users can freely select all relevant process variables of all or selected bioreactors to be displayed in one chart. There are no limitations in the number of process parameters. When using DASware analyze, the displayed process parameters even include integrated data from third party laboratory devices, such as nutrient analyzers, mass spectrometers or Raman. The software features tables and trend graphs of all measured values. It continuously logs and stores all data and events. These data are immediately accessible for evaluation using e.g. Microsoft Excel. Advanced charting functionalities ease further data analysis and comparison of runs.

Do I need programming skills to automate my process in DASware control?

DASware control includes a set of easy to implement automation features without any need of programming. Profiles and triggers allow for various control strategies, all of them easy to select by mouse click with parameters entered manually or just copy-pasted. Users with programming skills and special control requirements, however, are free to run scripted control strategies and profiles as well.

Can I change triggers, profiles, cascades and scripts online in DASware control?

Set-points, profiles, cascades, and scripts are online editable in DASware control. Offline values and events can be entered during or after the run and documented with a time stamp. These time stamps can be set individually, e.g to meet the time, when the sample was taken. This provides users with great flexibility to interact with the control system and reconsider parameter settings even in highly automated processes. All events, including online changes, are recorded in a SQL Server database.

How difficult is it to modify a DASGIP system subsequently?

Due to the unique modular design of the DASGIP Parallel Bioreactor System upgrades and modifications can easily be done: Functions like OD monitoring, exhaust analysis or extra feed lines can be added as modules to the control system. It is just as easy to substitute the bioreactor type or to upgrade a 4-vessel system to eight vessels. Actually, system modifications are that simple, some customers even share specific modules. For instance, the DASGIP GA4 exhaust analyzer is renowned for being moved from one bioreactor system to another. Like this, it delivers reliable in-depth analysis of OTR, CTR, and RQ for many bioreactors on demand.

How many speed-controlled pumps do the DASGIP bioreactors feature?

Up to eight peristaltic speed-controlled pumps per vessel offer continuous flow rates from 0.3 mL/h - 5 L/h. Each pump can be operated independently. Two different pump head sizes for the multi pump modules DASGIP MP4 and MP8, fitting various tube sizes make the DASGIP multi pumps a flexible and accurate solution.

How are DASGIP vessels agitated?

DASGIP bioreactors feature powerful overhead drives with agitation speeds ranging 30 – 1,600 rpm. The DASGIP TC4SC4 controls temperature and agitation in up to four bioreactors. Users can individually define each outlet set-point as well as choose from different operation modes (e.g. continuous or interval agitation).

Can I integrate DASGIP with my existing historians & supervisory control?

Yes, with the software DASware connect an optional server allows corporate-wide exchange of all process data via seamless integration with historians and supervisory control systems such as Delta V, OSI Pi, etc. This includes events, set-up information, and system status.

How can I harness data generated by the DASGIP system?

With DASware control all data and events are logged in one central SQL Server database. Further features such as export into Excel, PDF, and other file formats via one mouse click and strong charting capabilities ease plotting and data analysis. To harness data from numerous projects, on the long-run advanced archiving capabilities and data retrieval is required. DASware discover offers comprehensive and user-defined archiving functions and data retrieval for powerful data comparison and analysis. Users can, for example, link the utilized strain or cell line with its media composition, the controller setpoints and feeding profiles as well as with achieved product yields or viable cell densities to select for the best optimized combination and process.

Does DASware control give any alarms in case of critical deviation?

Critical deviation of process parameters such as pH, DO, redox, and temperature are color-coded in the software and saved in the event log. DASware control professional additionally allows notification of supervisory control systems or mobile communication tools via SMS or e-mail.

Can you help adjusting my controller loop parameters in DASware control?

DASGIP controllers are PI controllers, which are configured with default controller parameters adapted to the type of vessel and default experimental requirements. Depending on special customer requirements it might be necessary to modify these parameters. Our service will be happy to help you finding the right settings.

How to calibrate a pH sensor in DASware control?

DASware control software allows for parallel calibration of all sensors used within one system with all data logged in the central database together with set-points, profiles, cascades, process values, user interactions and other events. Good maintenance will prolong the sensors' lifetime whereas high temperatures (i.e. during autoclavation), alkaline solutions (i.e. during alkaline deactivation), repeated etchings and improper maintenance will reduce the electrode's lifetime.

How reliable are exhaust data when working at benchtop scale?

Exhaust oxygen and carbon dioxide concentrations of multiple bioreactor units are continuously monitored with the DASGIP GA exhaust analyzer. Together with the mass flow measurement and the input values of oxygen and carbon dioxide concentrations, automatic calculation of oxygen transfer rate (OTR), carbon dioxide transfer rate (CTR), and respiratory quotient (RQ) is possible, permitting direct conclusions on the metabolic state of a culture. As the DASGIP GA considers humidity, volume, and total flow typical sources for inaccurate OTR and CTR data in small working volumes are eliminated.

Is it possible to automatically control the foam in DASGIP vessels?

All DASGIP vessels can be equipped with foam control components. All you need is a level/foam sensor connected to the DASGIP sensor module (e.g. PH4PO4L) and a feed line for dosing of the antifoam agent. Now foam formation can be detected by the level sensor and antifoam agent is automatically pumped into the vessel until all foam is collapsed. The DASware control software allows any feed line to be used for antifoam or level control. Since medium composition has a strong impact on the electrical conductivity of medium and foam, every conductivity-based level sensor needs to be adjusted to foam and medium conductivity. DASware control supports monitoring conductivity and free adjustment of the conductivity threshold.

What maintenance is required for Eppendorf bioprocess systems?

As all complex technical systems Eppendorf bioprocess equipment should be maintained regularly to keep all parts in good condition. This maintenance avoids cost-intensive down times and contributes to preservation of value. A complete preventive maintenance is recommended once a year. Additional maintenance actions are recommended to be executed by the user prior to every run or in regular cycles (e.g. every month). Our service will be happy to advice.

What do I have to consider when returning equipment for repair?

Equipment returned to Eppendorf for service or any other reason needs to be decontaminated first. Please fill out the Decontamination Form that you will find in the “Service & Support” section of the Eppendorf website and attach it to the package. Equipment which was sent without the completely filled form cannot be handled. This form must also be completed before any on-site service, whether covered under warranty or not, can commence.

What does your warranty cover?

Eppendorf issues a warranty of one year from the day of delivery on its products in accordance with our terms and conditions. Excluded are consumables such as cables, tubing, glassware, and sensors as well as used material.

Does Eppendorf offer bioreactor training?

We highly recommend a training at the customer’s site for all new customers. Besides this initial training, Eppendorf also offers individual trainings adapted to the user’s requirements like training of new employees or advanced training/recapitulation for employees already having experience with DASGIP or DASbox systems. All trainings are performed at the customer’s site using the customer’s equipment to have the training focused on the customer’s demands. The structure and methods of the training can be chosen to the customer’s requirements from general overview to detailed sessions on specific products or issues. Training in small groups is as possible as personal guidance.

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DASGIP^® Parallel Bioreactor System

Informações do produto

O DASGIP Parallel Bioreactor System é um sistema de biorreator para pesquisa e desenvolvimento de processos em volumes de trabalho de 200 mL a 1,8 L. Pode operar até 16 vidros ou BioBLU^® Single-Use Bioreactors em paralelo e é adequado para fermentação microbiana e cultura celular, incluindo o cultivo de células estaminais. A concepção modular permite aos usuários escolher a configuração mais adequada às suas necessidades de aplicação.

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Aplicações

Recursos

O DASGIP Parallel Bioreactor System possibilita um desenvolvimento de processos altamente eficiente, realizando o controle preciso dos parâmetros críticos do processo, a comparação rápida de numerosas condições experimentais e a utilização ideal de todos os dados gerados.
• O funcionamento paralelo de até 16 biorreatores economiza tempo e espaço e melhora a reprodutibilidade.
• Fácil conversão de biorreatores de uso único para biorreatores reutilizáveis e vice-versa.
• O conjunto de software DASware^® possibilita o monitoramento e o controle precisos dos parâmetros críticos do processo, bem como a implementação de circuitos de feedback personalizados, estratégias de alimentação e outras caraterísticas.
• A integração perfeita com o Bioprocess Autosampler proporciona a amostragem automatizada 24 horas por dia, 7 dias por semana, a fim de obter conjuntos de dados completos.
• Conecta-se ao software BioNsight^® cloud para monitoramento remota do processo e análise simples e remota de todos os conjuntos de dados, disponibilizando as informações necessárias para a devida tomada de decisões.

Funcionamento de vários biorreatores em paralelo

• O DASGIP Parallel Bioreactor System foi concebido como um sistema quádruplo que pode ser expandido para até 16 biorreatores funcionando em paralelo.
• Os pontos de ajuste, as estratégias de controle e o tempo de inoculação de cada biorreator podem ser controlados individualmente a partir de uma instalação de software. Desta forma, podem ser testadas várias condições do experimento em paralelo.

Automatizar a amostragem de bioprocessos

• O sistema de biorreatores pode ser perfeitamente integrado com o Bioprocess Autosampler, que coleta amostras de vários biorreatores 24 horas por dia, 7 dias por semana, em intervalos curtos e regulares, e as conserva temperadas para análise posterior.
• O Bioprocess Autosampler pode ser instalado com sistemas de controle de biorreatores novos ou adaptados a sistemas de controle de biorreatores existentes.

Adequado para cultura celular e fermentação microbiana

• O DASGIP Parallel Bioreactor System adapta-se às necessidades de diferentes tipos de células, incluindo bactérias, leveduras, células de insetos, células diferenciadas de mamíferos e células estaminais.
• Os dois modelos são adaptados para cumprir os requisitos de fluxo de gás de aplicações microbianas e de cultura celular, respectivamente.
• O DASGIP Parallel Bioreactor System é compatível com os biorreatores concebidos para atender as necessidades das culturas microbianas e celulares, respectivamente.

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Especificações	DASGIP^® Parallel Bioreactor System	DASGIP^® Parallel Bioreactor System

Aplicação	Cultura celular	Microbiologia
Número de unidades paralelas	Até 16	Até 16
Software	Controle DASware, outro DASware opcional	Controle DASware, outro DASware opcional
Interface do usuário	Computador de processo com monitor	Computador de processo com monitor
Consumo de energia típico (sistema de 4 dobras sem computador de processo)	480 W (a 230 V)/ 432 W (a 115 V) (595 W/552 W sem DASGIP Bioblock)	541 W (a 230 V)/ 467 W (a 115 V) (639 W/584 W sem DASGIP Bioblock)
Dimensões (W × D × H, sistema de 4 dobras sem computador de processo)	140 x 70 x 53 cm (55 x 28 x 21 in)	140 x 70 x 53 cm (55 x 28 x 21 in)
Peso típico	78 kg (60 kg sem DASGIP Bioblock)	96 kg (78 kg sem DASGIP Bioblock)
Bactérias/levedura/fungos	sim	sim
Mamífero/Animal	sim	sim
Inseto	sim	sim
Vasos intercambiáveis	sim	sim
Validável	não	não
Células-tronco	sim	sim

Bioreatores
Recipientes	Recipientes de vidro/descartáveis	Recipientes de vidro/descartáveis
Esterilização	Autoclavável/ pré-esterilizado	Autoclavável/ pré-esterilizado
Volume total	1,5 L e 2,3 L	1,3 L e 2,1 L

Agitação
Acionamento	Acionamentos superiores direto (recipientes de vidro)/magnéticos (descartáveis)	Acionamentos superiores direto (recipientes de vidro)/magnéticos (descartáveis)
Faixas de velocidade	30 – 1.250 rpm (padrão)/ 100 – 1.600 rpm (opcional)	100 – 1.600 rpm (padrão)/ 30 – 1.250 rpm (opcional)
Impelidores	Pás inclinadas	Tipo Rushton

Gaseificação
Alimentação de gás	TMFC; gaseificação da superfície e/ou aspersor	TMFC/rotâmetro; aspersor
Taxas de fluxo de gás padrão	0,1 – 50 sL/h, 0,1 – 40 sL/h CO₂ (DASGIP MX4/4, disponíveis outros módulos)	0,5 – 250 sL/h, 0,5 – 150 sL/h CO₂ (DASGIP MX4/4H, disponíveis outros módulos)
Mistura de gás padrão	Ar, N₂, O₂ e/ou CO₂	Ar, N₂, O₂ e/ou CO₂

Alimentação
Linhas de alimentação por vaso	Até 8	Até 8
Taxas de alimentação padrão (dependendo do diâmetro do tubo)	0,3 – 9,5 mL/h a 13 – 420 mL/h (DASGIP MP8) 10 – 70 mL/h a 0,4 – 5 L/h (DASGIP MP4)	0,3 – 9,5 mL/h a 13 – 420 mL/h (DASGIP MP8) 10 – 70 mL/h a 0,4 – 5 L/h (DASGIP MP4)

Monitoramento e controle
Regulagem de temperatura	Aquecimento e refrigeração integrados no DASGIP Bioblock (dedos de refrigeração adicionais opcionais)	Aquecimento e refrigeração integrados no DASGIP Bioblock (dedos de refrigeração adicionais opcionais)
Faixa de temperatura padrão (a 25° C de temperatura ambiente)	5 K acima da temperatura do agente de refrigeração – 99°C (DASGIP Bioblock)¹⁾	5 K acima da temperatura do agente de refrigeração – 99°C (DASGIP Bioblock)¹⁾
Controle de pH	−	Ácido e/ou base, e outras configurações
Controle de OD	Cascata (concentração de O₂, taxa de fluxo de gás) e outras configurações	Cascata (velocidade de agitação, concentração de O₂ opcional, taxa de fluxo de gás opcional) e outras configurações
Medição de ORP (redox)	-	Opcional
Nível/espuma	Opcional	Opcional
Medição de OD	Opcional (DASGIP OD4)	Opcional (DASGIP OD4)
Condensação de exaustão	Resfriado a água ou sem líquido (Peltier c/ DASGIP EGC4)	Resfriado a água ou sem líquido (Peltier c/ DASGIP EGC4)
Análise de exaustão	Opcional (DASGIP GA4)	Opcional (DASGIP GA4)
1)Aplicam-se as condições específicas da aplicação.

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DASGIP® Parallel Bioreactor System