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Centrifugation Applications
Lab Academy
- Cell Biology
- Centrifugation
- Centrifuges & Rotors
- Workflows
Centrifuges are used for many separation processes in the laboratory. The centrifuge works on the sedimentation principle: substances are separated according to their density under the influence of g-force. Depending on the sample type, there are several centrifugation methods available. Examples include isopycnic, ultrafiltration, density gradient, phase separation, and pelleting.
Workflows
DNA extraction - typical workflow via kit purification
Plasmid Mini-Prep* from E.coli over night culture (5mL)
Plasmid Maxi-Prep from E.coli overnight culture* (Commercial Kit protocol)
Protein extraction using TRIzol® Reagnet*
Preparation plasma, platelet, serum
Workflows
One of the most common methods of centrifugation is pelleting, which is used for many popular purification protocols, such as:
You can find some typical application protocols in our "basics in centrifugation" section. In each case, centrifugation, centrifuge accessories, centrifuge function and rotor specifications are stated.
- DNA extraction
- Plasmid DNA extraction (mainly bacteria or yeast)
- Protein extraction
- Extraction of blood cells
You can find some typical application protocols in our "basics in centrifugation" section. In each case, centrifugation, centrifuge accessories, centrifuge function and rotor specifications are stated.
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References:
Trizol® is a registered trademark of Fisher Scientific *:
Trizol® is a registered trademark of Fisher Scientific *:
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