For inactivating prions, can the rotors of the Centrifuge 5430/ 5430R be autoclaved at 135 °C ?

The standard rotor FA-45-30-11 can be autoclaved at 135 °C.

Sometimes my Centrifuge 5430/ 5430 R displays "EP". What does that mean?

"EP" is displayed when the automatic standby function is active. It is not an error code. If the centrifuge remains unused for more than 20 min., the standby function is activated, indicated by "EP" on the display. By using the standby key, the centrifuge is re-activated and ready to use. The letters "EP" were chosen because a lot of Eppendorf products already have this prefix in their name.

What tubes can be centrifuged with the Centrifuge 5430/ 5430 R?

Due to the different rotors and adapters, a wide variety of tubes and plates can be centrifuged with the Centrifuge 5430/ 5430 R: • Micro test tubes (0.5 - 2.0 mL) • PCR strips and 0.2 mL PCR tubes • Spin columns • Cryo tubes • Conical tubes (15 mL and 50 mL) • Blood collection tubes • Oak Ridge Tubes (10 mL, 30 mL and 50 mL) • PCR plates (96 and 384 well) • Micro test plates • Deepwell plates (up to 29 mm height) • Slides

What is the temperature range of the Centrifuge 5430 R?

With the Centrifuge 5430 R it is possible to set the temperature within a range from -11 °C to +40 °C.

What is the benefit of a so-called Kit rotor?

When working with nucleic acid purification kits with spin columns by certain manufacturers, sometimes tube lids cannot be closed any more. This means you have to centrifuge with open tube lids. These tube lids might rip off during the stress of centrifugation and might damage the centrifuge. In order to prevent this, the kit rotor with the raised rotor rim was designed. Open tube lids, supported against the rim, will not rip off during centrifugation. Another feature of this rotor is the enlarged distance between the rotor bores. The enlarged distance between the bores offers a very convenient handling when loading / unloading the rotor. Especially when working with long spin columns (e. g. from Princeton Separations) you will need a larger distance between the bores to load the rotor fully and make use of the complete rotor capacity.

Can the Centrifuge 5430/ 5430 R be opened in case of a power failure?

Yes, in the event of a power failure, the unit can be opened by means of the rotor key. Before the centrifuge is opened, always remove the mains plug from the plug socket and wait for the rotor to come to a standstill. For the emergency release of the centrifuge lid, the small plastic caps on the left-hand and right-hand side of the Centrifuge 5430 have to be removed. Then insert the rotor key into the nut underneath the cap and open the lid latches by turning in anticlockwise direction.

What is the meaning of the red knob on the rotor lid?

A fixed-angle rotor with a red knob on the rotor lid indicates aerosol-tightness.

What can I use the "Fast Temp pro" function for?

With the "Fast Temp pro" function it is possible to automatically start the "Fast Temp" temperature control run at a predetermined time. In order that the Centrifuge 5430 R already shows the desired temperature at the start of work, one can set a point in time in the device menu at which the temperature control run should be started. The rotor then begins to spin in the centrifuge at a rotor and temperature-specific rotational speed in order to quickly reach the set nominal temperature. This function can thereby be programmed in such a way that it is carried out once or several times. You can find a more detailed description in the operating instructions of the Centrifuge 5430/5430 R.

Which material is the standard fixed-angle rotor for micro test tubes made of?

The aerosol-tight standard fixed-angle rotor is made of massive, anodized aluminum. This material is durable and has got a very good chemical resistance due to the special coating. The aerosol-tight rotor lid is made of the same material.

Can the Centrifuge 5430/5430 R be operated in the cooling room at 4° C?

The Centrifuge 5430 can be operated at an ambient temperature in the range from 4° C to 40° C. Operation in the cooling room at a temperature of down to 4° C is therefore possible. The permitted range of the ambient temperature is between 10 °C and 40 °C for the Centrifuge 5430 R, so that this centrifuge may not be operated in a cooling room at 4 °C.

Why are the standard rotors in the micro centrifuges 5418 R, 5420, 5424 R, 5425, 5427 R, 5430 and 5430 R aerosol-tight?

At Eppendorf, the safety of the user always has the highest priority. That is why we now fit our centrifuges with aerosol-tight rotors as standard – at no extra charge.

How can the Centrifuge 5430/ 5430 R be cleaned?

The Centrifuge 5430/5430 R can be cleaned with neutral detergents. For disinfection, we recommend alcohol or alcoholic disinfectants. Make sure that the relevant legal requirements and directives are observed when choosing the disinfection method.

Does the Centrifuge 5430/ 5430 R recognize an inserted rotor automatically?

The Centrifuge 5430/ 5430 R has got an automatic rotor recognition, so that a newly inserted rotor is recognized automatically as soon as a run is started. The maximum speed will be automatically limited for each rotor. This prevents overspeeding of the rotor.

What tubes do you recommend for centrifugation at 30,000 x g?

The specifications of the tube manufacturers on the centrifugation resistance must generally be taken into consideration for the centrifugation of tubes of all kinds. This applies in particular for centrifugation at 30,000 x g, as only very few micro test tubes can withstand these extreme gravitational forces to date without breaking. The following tubes can, for example, be centrifuged at 30,000 x g: • Eppendorf Safe-Lock Tubes 0.5 mL • Eppendorf Safe-Lock Tubes 1.5 mL • Eppendorf Tubes 3810X 1.5 mL

When does the opening of the centrifuge lid take place automatically or manually?

Following the end of a centrifuge run, only the lid of the Centrifuge 5430 is automatically opened in order to provide quicker access to the sample and to avoid potential heat accumulation. When working with radioactive material, this function should be deactivated in the device menu and the centrifuge lid manually opened upon conclusion of the centrifugation run. The Centrifuge 5430 R does not come equipped with the option of automatic or manual opening of the centrifuge lid. In order to maintain a consistent temperature in the rotor chamber and in the sample, the centrifuge lid for this device must always be manually opened by pressing the "open" key.

The "Fast Temp" function is a temperature control run of the Centrifuge 5430 R without samples and with a rotor and temperature-specific rotational speed in order to quickly bring the rotor chamber, incl. the rotor, buckets and adapters, to the set nominal temperature. In order that the samples can be centrifuged from the beginning on at the desired temperature, one starts this temperature control run during sample preparation by pressing the "Fast Temp" key. In addition to the current temperature and the g-count (rcf) / rotational speed, the remaining duration of the temperature control run also appears in the display, so that one can more precisely plan the subsequent workflow. You can find a more detailed description in the operating instructions of the Centrifuge 5430/5430 R.

What is the lowest temperature that can be reached with the Centrifuge 5430 R?

The lowest temperature of the Centrifuge 5430 R is -11 °C. The device is thereby optimized with all rotors in such a way that at least 4 °C is maintained with all rotors at the appropriate maximum speeds.

Does the Centrifuge 5430 R has an energy saving function?

Yes, the ECO shut-off automatically switchs off the compressor after 8 hours of non-use. The device has a stand-by button.

When does the continuous cooling of the rotor chamber start?

The continuous cooling starts with each rotor standstill of the Centrifuge 5430 R in order to maintain the preselected nominal temperature of the rotor chamber not only during centrifugation. To this purpose the following prerequisites must be met: - The centrifuge is switched on. - The centrifuge lid is closed. - The nominal temperature is lower than the ambient temperature. - The centrifuge is not in standby mode. Continuous cooling is ended by opening the centrifuge lid or by pressing the standby key.

我想提高我的技能，你们能够提供哪些类型的培训或在线培训？

请访问 Eppendorf 网页关于 Eppendorf 培训中心的信息或联系您当地的 Eppendorf 客服，以获取更多信息和报价。

进行维护之前，需要我怎么样处理设备？

首先需要清除设备污染。设备不得锁定且所有样品已从设备中移除。

我可以自己进行软件更新吗？

编号对于带 USB 接口的离心机，应由有资质的 Eppendorf 维护技术人员通过现有 USB 端口进行软件更新。这样才能确保实现最新的安全要素、对转子进行最新检测并且通过最新参数优化机器运行。

转子检查是维护服务的一部分吗？

不是。可以在维护服务之外单独订购，具体取决于转子类型（固定角转子或水平转子）。

请联系您当地的 Eppendorf 服务团队。您可以在我们的主页或资讯材料中找到联系信息。

离心机需要多久维护一次？

这取决于使用强度，并且因设备负载而异。然而，建议每年至少维护离心机一次。

为什么要定期维护离心机？

为了确保设备的最优性能和精确度。这也能早期发现任何缺陷，然后可以很容易地纠正。执行大量离心任务会增加转子损坏的可能性。通过正规检查，可以在早期阶段检查出任何损坏，也就是说，可以在转子发生任何严重损坏之前更换。

离心机维护服务价格？

费用取决于型号和所执行的维护。请联系您当地的 Eppendorf 客服。

怎么样清洁离心机？

请使用中性清洁剂进行清洁。我们建议使用乙醇或醇类消毒剂进行消毒。请确保在选择消毒方法时遵守适用的法律法规和准则。您可以观看视频“Centrifuge Maintenance - The basis for your lab safety”（离心机维护 - 实验室安全的基础）或从我们的网站下载操作手册了解详细信息。

离心结束后，是否应该盖上冷冻离心机盖？

如果经常在低温下使用离心机，您应该盖上冷冻离心机盖。不使用时（例如夜间），请关掉离心机并清洁转子腔。离心机盖应保持打开状态以干燥离心机。

IQ 或 OQ 是什么？有什么用？

安装和操作验证；IQ 记录设备已正确交付和安装。 OQ 检查设备是否正常运行。 IQ/OQ 确保设备满足您的质量管理要求，确保设备正常运行且符合制造商指标。

离心机需要多长时间进行一次 OQ？

公司负责对设备进行技术检查。 Eppendorf 建议定期进行设备验证，具体取决于使用强度。然而，至少应每年进行一次 OQ。

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Centrifuge 5430/ 5430 R - 高速离心机

High-speed centrifuges Centrifuge 5430 and 5430 R

Eppendorf Centrifuge 5430 R with 12 centrifuge rotors

Rotary knob control on the Centrifuge 5430

Centrifuge rotor for microplates on Centrifuge 5430

Eppendorf Rotor A-2-MTP for Centrifuge 5430 with lid

Eppendorf centrifuge Rotor F-35-6-30 with 50 mL Conical tubes

Eppendorf centrifuge Rotor F-45-48-11 for Centrifuge 5430 R with lid

Eppendorf centrifuge Rotor FA-45-30-11 for Centrifuge 5430 with lid

Eppendorf Rotor FA-45-24-11-HS for Centrifuge 5430 with lid

Eppendorf Rotor FA-45-24-11-Kit for Centrifuge 5430 with lid

Eppendorf Rotor F-45-64-5-PCR for Centrifuge 5430 with lid

Eppendorf Rotor F-45-18-17-Cryo for Centrifuge 5430 with lid and cryovial adapters

Large backlit display on high-speed centrifuge Centrifuge 5430 R

产品信息

Centrifuge 5430和 5430 R 融合两种离心机的特色功能。这些高速离心机兼具微型离心机的小巧体积与多用途离心机的多样功能。

显示更多产品信息

您可以在以下网址找到其他下载资料本页面底部

产品信息

技术数据

特征

Centrifuge 5430 和 5430 R 集体积小巧的微型离心机与多用途离心机的特色功能于一身。这款高速离心机不仅搭载适用于 Eppendorf 试管和 PCR 管的离心转子，还搭载适用于微孔板和 15/50 mL 锥形管的离心转子。
转子程序中还提供额外的 Eppendorf QuickLock^® 离心转子，包括 48 孔转子（1.5 mL 或 2.0 mL）、16 孔转子Eppendorf Tubes 5.0 mL 和水平转子（24 x 1.5 mL 或 2.0 mL）。Centrifuge 5430和 Centrifuge 5430 R 具备无与伦比的多功能性，是我们Eppendorf微型离心机系列的旗舰产品。
按键式和旋钮式可选

操作简单安全

我们的气密性Eppendorf QuickLock转子只需转动 1/4 圈便可关闭。这不仅节省了您的日常工作时间，还可以减轻腕部压力。

体积小巧

压缩机位于 Centrifuge 5430 R 后面，冷冻款 Centrifuge 5430, 体积小巧，开盖高度低。

先进的操作系统

Centrifuges 5430和 5430 R 搭载先进的操作系统，具备更加节能的 ECO 智能关闭和FastTemp预冷功能。它们均搭载菜单驱动的多语言操作菜单以及大尺寸背光显示屏幕，可以保存多达 99 个用户定义程序和 5 个程序键，以方便访问常规程序。
Eppendorf高速离心机Centrifuge 5430 R不仅配备标准FastTemp预冷功能，而且搭载名为 FastTemp pro^® 的独特软件选项。FastTemp pro功能可根据预设时间和日期实现快速自动预冷。FastTemp pro 可以设置为特定日期，也可以设置为一周中指定日期的重复事件。当您离开实验室时，只需将离心机设置为待机状态， FastTemp pro 功能便可在次日早晨完成预冷。这不仅有助于简化操作流程；还可以整晚为您节省高达 90 % 的能耗。
ECO 智能关闭功能可在设备处于非活动状态 1/2/4/8 小时后关闭压缩机，整晚可节约 88 % 的能耗，并延长压缩机使用寿命。此功能可在菜单设置中停用。

您是否拥有 Eppendorf 产品？已经注册了？

您知道吗，每款 Eppendorf 注册产品均可在我们的奖励商店兑换以下?
• 100 点 epPoints^®
• 当您注册离心机、振动试验机、CO2 培养箱或循环仪时，可享受额外 3 个月的保修服务
• 每款注册产品均可参加一次 Eppendorf 独家抽奖活动
• 在 Eppendorf 网站上分享您的个人产品故事

操作非常简单！您可以 -
1. 下载 Eppendor f应用程序，扫描并注册，或者
2. 访问我们的网页，登录 myEppendorf 帐户并输入产品序列号
更多信息敬请访问：www.eppendorf.com/productregistration

废弃产品

包装处置
离心机包装包含不同材料，包括木制托盘、纸板、塑料防尘罩和一些泡沫部件。材料的回收变得越来越重要：您知道欧洲几乎回收所有的纸板材料吗？我们的离心机纸板包装含有约 50%的回收材料。请通过收集冰箱纸板包装材料并将其防止在适当的回收容器中，来支持回收有价值原材料的全球可持续发展倡议。对于LD-PE和泡沫制成的塑料防尘罩，我们建议选择可以回收PE材料的专门回收合作伙伴料。建议联系您当地的废物管理公司或设施管理团队，了解适合贵公司的可用回收方案。

仪器处置
我们的离心机可以使用多年。但如果需要更换，我们建议您根据当地要求，妥善处置此类仪器。我们强烈建议您在具有主动制冷仪器方面具有丰富经验的经认证本地回收合作伙伴。“本地”处理可减少运输影响。由于制冷剂需要以安全、可持续的方式进行移除和回收，建议采用“认证”方式对冷却离心机进行处置。

消毒
该设备用于实验室和/或用于处理生物样品。请记住对需要处理的设备进行充分去污。查看当地要求。有关更多信息，请联系当地的生物安全官员和/或废物管理官员。检查您当地的回收合作伙伴是否有特殊说明和/或文件要求。您也可以使用Eppendorf去污表格作为指导。

阅读更少

规格	5430	5430 R

最大 RCF	30130 × g	30130 × g
最大 RCF，带固定角转	30130 × g	30130 × g
最大 RCF，带水平转子	16049 × g	16049 × g
速度	100 – 17,500 rpm (100 rpm 步进)	100 – 17,500 rpm (100 rpm 步进)
产品类型	离心机	离心机
可用转子	12	12
最大容量	48 × 1.5/2.0 mL，6 × 50 mL，2 × MTP	48 × 1.5/2.0 mL，6 × 50 mL，2 × MTP
加速时间¹⁾	14 s	14 s
减速时间¹⁾	15 s	15 s
定时器	30 秒至99小时59 分钟, 可连续离心	30 秒至99小时59 分钟, 可连续离心
SOFT 软刹车功能	是	是
噪音水平	< 58 dB(A) ， F-45-30-11固定角转	< 54 dB(A)， F-45-30-11固定角转
电源	230 V, 50 – 60 Hz	230 V, 50 – 60 Hz
最大能耗	475 W	1.050 W
尺寸（长x宽x高）	34 × 42 × 25 cm / 11.2 × 16.3 × 9.8 in	38 × 64 × 29 cm / 15 × 25.2 × 11.7 in
产品重量	29 kg / 63.9 lb	56 kg / 123.5 lb
开盖高度	56 cm / 21.8 in	63 cm / 24.8 in
冷冻型	非冷冻	冷冻型
温度范围	–	-11 °C 至 +40 °C