Optimize Your Plasmid Yields - Key Success Factors in Production from Shaker Flask to Fermenter

Ines Hartmann – Application Specialist Cell Handling Eppendorf SE Lab Academy 28. septembre 2022

Recombinant plasmid DNA (pDNA) is produced in bacterial cultures, mostly in Escherichia coli (E. coli) in different scales from tube culture to fermenter. Successful pDNA production with consistent high yields and quality relies on multiple factors, including the vector type, the host strain, the insert, and the methodology chosen for cultivation and downstream purification.

In this webinar, we review the distinct steps in the plasmid production workflow and give tips on optimization: from vector and strain selection, to cultivation from shake flask to fermenter up to downstream processing like harvesting and purification. The focus will be on the optimization of the cultivation in shake flasks and fermenters.

Target groups:
Scientists and technical personnel in academic and industrial setting, as well as students who start or currently work on plasmid production and want to improve their plasmid yields in tube, shake or fermenter cultures.

Key learning objectives:

Learn about the different application fields of plasmid production
Get an overview on the basic steps involved in plasmid production
Learn what to consider in plasmid vector and host strain selection
Learn how to optimize your plasmid culture in tube and shake flask culture
Learn about stirred-tank fermenter culture set-up and scale-up considerations
Get advice on harvesting and purification

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Speaker

Ines Hartmann – Application Specialist, Cell Handling, Eppendorf SE

Stefan Schloesser – Scientific Communications Manager, Bioprocess Center, Eppendorf SE

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Optimize Your Plasmid Yields - Key Success Factors in Production from Shaker Flask to Fermenter

Speaker