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Optimize Your Plasmid Yields - Key Success Factors in Production from Shaker Flask to Fermenter

Ines Hartmann – Application Specialist Cell Handling Eppendorf SE Lab Academy

Recombinant plasmid DNA (pDNA) is produced in bacterial cultures, mostly in Escherichia coli (E. coli) in different scales from tube culture to fermenter. Successful pDNA production with consistent high yields and quality relies on multiple factors, including the vector type, the host strain, the insert, and the methodology chosen for cultivation and downstream purification.

In this webinar, we review the distinct steps in the plasmid production workflow and give tips on optimization: from vector and strain selection, to cultivation from shake flask to fermenter up to downstream processing like harvesting and purification. The focus will be on the optimization of the cultivation in shake flasks and fermenters.

Target groups:

  • Scientists and technical personnel in academic and industrial setting, as well as students who start or currently work on plasmid production and want to improve their plasmid yields in tube, shake or fermenter cultures.

Key learning objectives:

  • Learn about the different application fields of plasmid production
  • Get an overview on the basic steps involved in plasmid production
  • Learn what to consider in plasmid vector and host strain selection
  • Learn how to optimize your plasmid culture in tube and shake flask culture
  • Learn about stirred-tank fermenter culture set-up and scale-up considerations
  • Get advice on harvesting and purification
  • Live: “Ask the Expert” session – Deepen your knowledge and optimize your plasmid culture

Bio Speaker:

Ines Hartmann is an application specialist for cell handling and responsible for the shakers and incubators at the Eppendorf headquarter in Hamburg, Germany. She joined the company in 2008 and has worked on several topics in cell handling, liquid handling and consumables. She obtained her diploma degree in biology at the Humboldt University of Berlin, Germany and gained expertise in various laboratory techniques.

Register and watch recording now!