In the field of Molecular Biology the goal is to analyze and understand the molecular basis of biological processes. It concerns itself with the function of DNA/RNA sequences and the interaction between DNA, RNA and proteins. Furthermore, this field overlaps to a great extend with other areas of biology and chemistry, particularly biochemistry and genetics. One of the most important molecular biology methods is the polymerase chain reaction (PCR). It is used to exponentially amplify a specific segment of DNA for various downstream applications like DNA cloning (genetic engineering), gene expression analysis (RT-PCR, RT-qPCR), genetic fingerprinting (paternity testing, forensics), sequencing (Next generation sequencing) and others more.