What is the Tube Clip used for?

The Eppendorf Tubes ® 5.0 mL offer secure closure at incubation temperatures up to 80 °C. For higher temperatures, the Tube Clip should be used to secure the lid.

Can the universal adapter for conical 15 mL screw-cap tubes be used in each rotor/adapter that is designed for this tube type?

It can be used in the respective Eppendorf rotors/adapters, but we assume no liability for use in rotors/adapters from other manufacturers.

Is it necessary to buy new equipment to be able to use the new tube?

The bottom shape of the Eppendorf Tubes ® 5.0 mL is similar to that of conical 15 mL tubes which means that they are compatible with a wide range of standard laboratory equipment. In addition, an extensive range of accessories is available, enabling them to be used in many devices. Both racks and storage boxes are available for lab bench work and storage. Incubation and mixing operations can be performed using suitable blocks on Eppendorf thermomixers and compatibility with Eppendorf centrifuges is ensured by special rotors and a variety of adapters. With a suitable reservoir rack module, the tubes can also be integrated into the epMotion ® automated liquid handling workstation. A detailed description of the accessories is provided in Application Note No. 265

What types of applications can be carried out with Eppendorf Tubes ® 5.0 mL?

Basically, this tube type can be used for the same applications as the micro centrifugation tubes available in 1.5 and 2 mL formats. They close a gap between this small tube format and conical 15 mL tubes for sample volumes up to 5 mL. The tubes can be used in the field of cell culture. For this purpose, they are available in the purity grades “Sterile” (= sterile and pyrogen-free) or Biopur ® . They are suitable for lysis of cells and tissues and purification of nucleic acids and proteins. For this, the PCR clean purity grade and LoBind variants are provided. They are also ideally suited for the preparation of large volumes of master mixes, buffers or dilution series as well as for space-saving storage of samples.

Are the Eppendorf Tubes ® 5.0 mL available with the same purities and LoBind variants as the small micro centrifugation tubes from Eppendorf?

The Eppendorf Tubes ® 5.0 mL are available in the purity grades Eppendorf Quality, PCR clean and Biopur ® and also in the purity grade Sterile. In addition to irradiation for sterilization, this also includes certified testing for the absence of pyrogens. The tubes are also available as DNA LoBind and Protein LoBind variants.

Up to which g-force can the Eppendorf Tubes ® 5.0 mL be centrifuged?

All tube types are resistant up to 25,000 x g when used in combination with a suitable rotor or adapter. (Test conditions: 45° fixed-angle rotor at 40 °C, with aqueous salt solution (density 1.0 g/mL) and 90 mins). The mechanical loading capacity of the tubes is reduced by the use of organic solvents.

How resistant are the Eppendorf Tubes ® 5.0 mL to chemicals?

The tubes are made of high quality highly transparent polypropylene and therefore resistant to a variety of commonly used chemical substances in the laboratory. An overview is provided in Application Note No. 56.

Is it possible to store samples in 5.0 mL Eppendorf Tubes ® in the freezer?

Yes, the tubes can be stored down to -86 °C.

Are Eppendorf tubes and plates (also LoBind variants) resistant to 100% acetonitrile?

Eppendorf tubes and plates including LoBind variants are resistant against 100% acetonitrile only in room temperature conditions. Above 40°C and prolonged exposure (days) may lead to partial damage and is not recommended. For lower acetonitrile concentrations the resistance parameters will be better so prolonged exposure and higher temperatures may be possible. This must be however tested by the customer.

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Eppendorf Tubes^® 5.0 mL - 微型管

Eppendorf Tubes® 5.0 mL placed in centrifuge rotor buckets

Eppendorf microtubes® 5 mL placed in reservoir rack module

Eppendorf microtube® 5 mL next to 1.5 mL microtube and 15 mL conical tube

Eppendorf microtubes® 5 mL in high speed rotor

Eppendorf microtubes® 5 mL in amber with closed lid

Eppendorf microtube® rack with 5 mL and 1.5 mL tubes.

Eppendorf Microtube® 5 mL inside conical bottom centrifuge adaptor

5 mL microtubes in the Eppendorf ThermoMixer® C

Scientist pipetting into Eppendorf 5 mL microtubes® stored in rack

Eppendorf Tubes® 5 mL with screw cap and snap cap open

5 mL microtubes - screw cap, snap cap and amber

The ultimate screw cap microtube for sample security.

Screw cap microtube 5 mL with Eppendorf stamp

Eppendorf Storage Box: Space for up to 5 x 5 Eppendorf Tubes® 5 mL

Tubes 5.0 mL screw cap, amber and transparent

Light Protection as well as Transparency

Selection of Eppendorf tubes® including microtubes in 0.5 mL, 1.5 mL, 5 mL format, and conical tubes in 15 mL and 50 mL format

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使用中等容量，并且不确定要选择哪种微离心管？能够处理 0.5 mL - 5 mL的样品，Eppendorf Tubes 5.0 mL 填补替代离心管型号与锥形离心管之间的空白，容积更大，可达 5.0 mL。

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特征

忘记不切实际和不方便的样本量处理，并找到可以容纳高达 5 mL的正确试管。 Eppendorf微离心管是简单、安全的样品制备基准，可选择易于操作的卡扣盖或安全的旋盖。

品质无与伦比的优质微离心管

确保您的DNA和蛋白质数据尽可能可靠！ Eppendorf微离心管的制造不使用增滑剂、增塑剂和杀生物剂，以防止污染浸出物干扰定量分析。

图形显示在 90 °C条件下，使用不同厂商的离心管培养30 分钟后纯水的紫外吸收光谱。此数据使用的是5.0 mL 带锁扣盖型号。如需了解更多信息和所述规程，请参阅应用文献第264期。

5 mL微离心管，完整的系统

5 mL微离心管延续自历史达到50年的Eppendorf microliter system，其中包括1.5 mL微离心管，我们知名的Thermomixer，微离心机和Eppendorf移液器。基于这一已成熟并且广泛流行的理念，Eppendorf还提供5.0 mL 系统，具有与我们5 mL微离心管密切关联的组件，以确保简化您的工作流程。

• Eppendorf Tubes^® 5.0 mL
• 节约空间的Eppendorf 离心管架子和存储盒
• Thermomixer-兼容加热模块。
• 5 mL移液器（手动和电子式）以及epT.I.P.S.移液器吸头
• 通用离心机适配器，包括高速方案，用于现有电机
• 用于epMotion自动化工作站的适配器

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Eppendorf Tubes® 5.0 mL - 微型管

Tubes 5.0 mL with screw cap and snap cap ACT Label

Eppendorf Tubes® 5.0 mL placed in centrifuge rotor buckets

Eppendorf microtube® 5 mL tube clip

Eppendorf microtubes® 5 mL placed in reservoir rack module

Eppendorf microtube® 5 mL next to 1.5 mL microtube and 15 mL conical tube

Eppendorf microtubes® 5 mL in high speed rotor

Eppendorf microtubes® 5 mL in amber with closed lid

Eppendorf microtube® rack with 5 mL and 1.5 mL tubes.

Eppendorf Microtube® 5 mL inside conical bottom centrifuge adaptor

5 mL microtubes in the Eppendorf ThermoMixer® C

Scientist pipetting into Eppendorf 5 mL microtubes® stored in rack

5 mL Eppendorf microtube®, open

Eppendorf Tubes® 5 mL with screw cap and snap cap open

5 mL microtubes - screw cap, snap cap and amber

The ultimate screw cap microtube for sample security.

Screw cap microtube 5 mL with Eppendorf stamp

Eppendorf Storage Box: Space for up to 5 x 5 Eppendorf Tubes 5 mL

Tubes 5.0 mL screw cap, amber and transparent

Light Protection as well as Transparency

Selection of Eppendorf tubes® including microtubes in 0.5 mL, 1.5 mL, 5 mL format, and conical tubes in 15 mL and 50 mL format

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Tubes 5.0 mL

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品质无与伦比的优质微离心管

5 mL微离心管，完整的系统

Eppendorf Tubes^® 5.0 mL - 微型管