How can Multipette devices be calibrated?

To calibrate Multipette in accordance with EN ISO 8655, three different volumes with ten measured values each have to be checked. These three volumes are the nominal volume (= the maximum dispensable volume), 50% of the nominal volume and 10% of the nominal volume of the Combitips advanced used. The checking procedure, the test conditions, and the limits in accordance with EN ISO 8655 are described in "Eppendorf SOP" for Manual Dispensing Systems.

How should warm or cold liquids be pipetted?

Best Practices for Pipetting Warm or Cold Liquids: Warm and cold liquids can behave differently than those at room temperature, requiring adjustments in pipetting techniques. Temperature differences between the liquid and the pipette can affect the air cushion, resulting in inaccuracies and imprecision that may exceed permissible error limits. To achieve optimal results, ensure that the pipette, tips, liquid, and surrounding air are all at the same temperature. For enhanced accuracy and precision, consider using a positive displacement device like the Multipette M4 or E3/E3x.

What does EN ISO 8655 cover?

ISO 8655-2022 is the global standard for piston-operated volumetric apparatus (POVA), including pipettes, burettes, dilutors, dispensers, and manual precision syringes, as defined by the International Organization for Standardization (ISO). It ensures standardized performance for manufacturers, calibration providers, and users.It is structured in 10 chapters: ISO 8655-1: Terminology, general requirements and user recommendations ISO 8655-2: Pipettes ISO 8655-3: Burettes ISO 8655-4: Dilutors ISO 8655-5: Dispensers ISO 8655-6: Gravimetric reference measurement procedure ISO 8655-7: Alternative measurement procedures ISO 8655-8: Photometric reference measurement procedure ISO 8655-9: Syringes ISO 8655-10: User guidance, user qualification and POVA qualification.

What do the terms random error and systematic error mean in a pipette calibration?

In pipette calibration, random error and systematic error are two key concepts that help ensure accuracy and precision in measurements. Random Error refers to the variability in measurements when the same volume is dispensed multiple times. It is also known as imprecision and is determined by calculating the repeat standard deviation of 10 measurements. These errors tend to scatter around the true value and can be minimized by consistent technique and proper training. Systematic Error is the consistent deviation of the measured volume from the selected/true volume, also known as inaccuracy and is determined by establishing the mean value of 10 measurements. Systematic errors can arise from defective or misadjusted devices and need to be corrected through proper calibration and maintenance.

Can the Multipette M4 and E3/E3x be autoclaved?

No, the Multipette M4 and E3/E3x cannot be autoclaved. The device can be decontaminated using a cloth moistened with 70% isoproanol as disinfectant. Then moisten the cloth with water, wipe down the housing and remove residual cleaning agent. Please ensure that no moisture can get into the device during cleaning.

Why can Multipette M4 and E3/E3x only be operated with Combitips advanced?

When using a positive displacement system, the dispenser and the dispenser tip must be perfectly matched. This will ensure that the high demands with regard to precision and accuracy are reliably met. Combitips advanced are perfectly matched to Eppendorf Multipette M4 and E3/E3x and M4. Combined with the center sleeve of the devices, accurate dosing for dispensing is supported in particular.

What is the maximum number of dispensing steps that can be carried out with the Multipette M4?

In position 0.5 of the selection dial, 100 dispensing steps can be performed with the Multipette M4.

What is the volume range covered by the Multipette M4 when Combitips advanced are used?

Eppendorf offers 9 different sizes of Combitips advanced. This enables the Multipette M4 to cover a dispensing volume of 1 µL - 10 mL.

What material is the Multipette M4 and E3/E3x made of and what chemicals are they resistant to?

Please refer to the Operating Manuals on our homepage for detailed information on materials. The Multipette M4 has a chemical resistance sheet.

Does the Multipette M4 also work without a battery?

Yes, the Multipette M4 also works with an empty battery or without a battery. The precision of the dosing is due to the reliable mechanics of the Multipette M4 and will not be affected if the battery fails. Use the volume in the Operating Manual table to set the volume.

How much does the Multipette M4 weight?

The Multipette M4 only weighs 105 g.

What does the “PhysioCare concept” symbol on the Multipette M4 and E3/E3x mean?

The symbol means that the Multipette M4 and E3/E3x have been developed in accordance with the considerations of the Eppendorf PhysioCare concept. With its three areas, the concept includes the whole range of ergonomics, not just a single element. Instead of focusing on a particular product feature, the products with the PhysioCare symbol provide a holistic solution. Further details on the PhysioCare Concept are available at www.eppendorf.link/physiocare.

Is it possible to attach the Multipette M4 to a wall?

Yes. The Multipette M4 comes with a holder which can be inserted in a pipette carousel or it can be fixed to a clean glass or ceramic surface using the enclosed adhesive strip.

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Multipette^® M4

Multipette® M4 multi-dispenser with an assortment of fitting Combitips® advanced positive displacement pipette tips

Eppendorf旋转式移液器支架2或旋转式充电支架2可以安全存放您的Multipette® M4多道分液器。

Multipette® M4 Multi-Dispenser Held By Scientist (Product Close-Up)

Female and Male Scientists Working with Multipette® M4 and Eppendorf Conical Tubes

ACT label of sustainability certification for Multipette® M4 starter kit

产品信息

彻底告别耗时费力的连续分液工作！Multipette M4移液器——Eppendorf专业之选，无需反复多次抽吸液体，十分适合步骤较多的连续分液任务。搭配Combitips^® advanced分液管， Multipette便可轻松处理传统移液器难以移取的粘性、挥发性或发泡液体。Multipette/Combitips正位移分液系统采用密封吸头，可确保在处理传染性、毒性或放射性液体时高度安全。

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产品信息

技术数据

用途

特征

一次吸液便可完成多达百次分液

Multipette M4手动分液器可实现“一次吸液、百次分液”，是长时间重复精准分液的理想选择，如离心管或工作板加样、试剂等分等。液体通过外置活塞吸入和分配，这意味着没有可能受液体样品特性影响的气体活塞。这可确保始终分配正确的体积——无论液体的密度、粘度和挥发性如何。
与分液器相配套的Combitips^® advanced与ViscoTip^®正位移分液管可被自动识别，省去耗时的体积换算步骤；且分液管为密封结构，可充分保障操作人员、仪器与样本安全，是处理感染性、有毒性或放射性液体的理想选择。

Eppendorf于1978年率先推出Multipette/Combitips多次分液系统，并在此后对其进行多次迭代升级。这是全球首款实现“一次吸液、多次分液”的移液系统。其设计至今仍是提升液体处理效率的核心方案，广泛应用于大量使用微孔板或系列试管的实验室。目前推出的MultipetteM4型号是热销款Multipette plus手动分液器的升级版。2024年，EppendorfMultipette M4多道分液器与Combitips advanced分液管共同获颁ACT^®环境影响因子标签，彰显了我们对绿色制造的承诺，以及协助客户做出更环保购买决策的决心。

此款连续移液器配备先进操作界面和传感器控制器，操作便捷

步骤计数器
• 显示已执行完成的分液步骤数

体积选择刻度盘
• Combitips advanced共有20种不同体积设置，适用于不同尺寸

运动传感器
即使Combitips advanced分液吸头没有弹出，Multipette M4也会在不使用时自动关机以节省电力。当再次移动时，它会自动启动，并且“记住”执行的分液步骤数，以提高效率。

Combitips advanced装卸传感器。
• 自动Combitips advanced吸头识别和体积显示
• 确保Combitips advanced和Multipette M4之间的安全连接

让耗时费力的连续移液工作变得更加轻松舒适

Multipette M4配备易于操作和易于调节的体积选择刻度盘，有助轻松管理连续移液工作。采用符合人体工程学的扶手设计，带来放松的手部姿势并且易于操作，确保Multipette M4为全天工作提供更高水平的舒适度。

操作杆形状设计符合人体工学，单手即可完成吸液、分液以及Combitips advanced分液管脱卸操作。

如何使用Multipette M4连续移液器？

Multipette M4的操作使用十分简单：只需将适配的分液吸头（Combitips advanced或ViscoTip）插在M4上，同时按下操作杆即可。移液器会自动识别吸头大小，接着您便可以直接选择满足您应用需求的分液体积和步骤。不同分液步骤的体积设置可以更改。将吸头浸入液体样品中，轻轻按压操作杆，吸取需要分配的总体积。再次按压操作杆，启动反向排液，以最大限度提高准确度。然后缓慢推动操作杆，直至无法推动为止，即可开始向目标容器中分配样品。如需了解更多信息，请参阅《快速入门指南》。

多道分液移液器便捷收纳方案

Eppendorf Multipette M4壁挂式移液器支架或Eppendorf Pipette Holder System既可固定于几乎任何平面，也可搭配充电式或非充电式手动移液器旋转支架使用。此外，一支Multipette M4多道分液器还可借助Pipette Stand 2（Eppendorf）进行收纳。

处置生命周期结束后的包装和分液器

Multipette M4分液器包装含有纸板、塑料薄膜等不同材料。我们的 Multipette包装用纸板含有约 92 %的回收材料。请收集包装材料并分类放于贵司废物收集容器，以实际行动支持回收有价值原材料的全球可持续性倡议。对于采用LD-PE制成的包装薄膜，我们建议选择一位专门回收聚乙烯材料的合作伙伴。

Eppendorf液体处理仪器的设计使用年限长达数年。如果需要处置Multipette，请注意它们包含电子元件和电池并可能已被污染。请联系您所在地的生物安全官员和/或废品管理机构，了解电子废物和实验室废物的处置要求与理想做法。如有文件要求，您可以将《Eppendorf消毒证书》作为指导。

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Eppendorf Multipette^® M4连续移液器技术参数与规格，包括量程范围、电池类型、产品重量等更多信息。

规格	Multipette^® M4 启动套装	Multipette^® M4

ACT 标签	是	是
产品类型	分液器	分液器
移液类型	外置活塞系统	外置活塞系统
通道数量	1-通道	1-通道
体积范围	1 µL – 10 mL	1 µL – 10 mL
操作模式	手动	手动
随机测量误差	see Combitips advanced	see Combitips advanced
系统测量误差	see Combitips advanced	see Combitips advanced
高温高压灭菌	否	否
电池类型	纽扣电池	纽扣电池
Consumables	Combitips^® advanced, ViscoTip^®	Combitips^® advanced, ViscoTip^®
开放系统（第三方耗材）	否	否
产品重量	105 g	105 g
交付内容	多道分液器，电池（已插入），操作手册，Combitips^® advanced Box 2.0分液管，Combitips^® advanced 混合装（每种尺寸分液管各1个），Pipette Holder 2 移液器支架	多道分液器，电池（已插入），操作手册，Combitips advanced 2.5 mL分液管，Pipette Holder 2移液器支架