Can the Multipette E3/E3x be used in a cold room?

The Multipette E3/E3x can be used in a cold room down to 5°C. Please note that the technical data for precision and accuracy only applies to the temperature range described in the Eppendorf SOP in compliance with the ISO 8655.

What is the difference between Multipette E3 and Multipette E3x?

The Multipette E3 has three functions: dispensing, automatic dispensing and pipetting. The Multipette E3x has four additional functions: sequential dispensing, aspiration, aspiration/dispensing, and titration. Both models use Combitips advanced.

Is it possible to adapt the aspiration and dispensing speed using the Multipette E3/E§x??

Yes, the aspiration and dispensing speed can be set to 8 different speed levels using theMultipette E3/E3x.

How long does it take to recharge the battery of a Multipette E3/x?

Charging the battery of a Multipette E3/x takes approximately two hours. It is possible to go on working while charging.

How large is the capacity of the fully charged battery of a Multipette E3/x? What is its lifetime?

The battery of a Multipette E3/xlife depends on various factors. The full battery capacity is sufficient for at least 500 aspirations and 10000 dispensing steps under the specified test conditions specified in the Instructions for Use. Under these conditions, the battery life is approx. 5 hours.

Are the Multipette E3/E3x stable to UV light?

The Multipette E3 and E3x are stable to UV light. In the event of prolonged irradiation with UV light, material discolorations may occur, but this does not affect the proper function of the Multipette.

How can Multipette devices be calibrated?

To calibrate Multipette in accordance with EN ISO 8655, three different volumes with ten measured values each have to be checked. These three volumes are the nominal volume (= the maximum dispensable volume), 50% of the nominal volume and 10% of the nominal volume of the Combitips advanced used. The checking procedure, the test conditions, and the limits in accordance with EN ISO 8655 are described in "Eppendorf SOP" for Manual Dispensing Systems.

Are your pipettes free of nucleic acids?

We cannot guarantee that our pipettes are completely free of nucleic acids. While we maintain high standards of cleanliness and care during production, we do not implement specific measures to prevent nucleic acid contamination. To protect your samples from potential contamination, we recommend using positive displacement devices or filter tips, such as ep Dualfilter T.I.P.S. or ep Dualfilter T.I.P.S. SealMax.

How should warm or cold liquids be pipetted?

Best Practices for Pipetting Warm or Cold Liquids: Warm and cold liquids can behave differently than those at room temperature, requiring adjustments in pipetting techniques. Temperature differences between the liquid and the pipette can affect the air cushion, resulting in inaccuracies and imprecision that may exceed permissible error limits. To achieve optimal results, ensure that the pipette, tips, liquid, and surrounding air are all at the same temperature. For enhanced accuracy and precision, consider using a positive displacement device like the Multipette M4 or E3/E3x.

What does EN ISO 8655 cover?

ISO 8655-2022 is the global standard for piston-operated volumetric apparatus (POVA), including pipettes, burettes, dilutors, dispensers, and manual precision syringes, as defined by the International Organization for Standardization (ISO). It ensures standardized performance for manufacturers, calibration providers, and users.It is structured in 10 chapters: ISO 8655-1: Terminology, general requirements and user recommendations ISO 8655-2: Pipettes ISO 8655-3: Burettes ISO 8655-4: Dilutors ISO 8655-5: Dispensers ISO 8655-6: Gravimetric reference measurement procedure ISO 8655-7: Alternative measurement procedures ISO 8655-8: Photometric reference measurement procedure ISO 8655-9: Syringes ISO 8655-10: User guidance, user qualification and POVA qualification.

What do the terms random error and systematic error mean in a pipette calibration?

In pipette calibration, random error and systematic error are two key concepts that help ensure accuracy and precision in measurements. Random Error refers to the variability in measurements when the same volume is dispensed multiple times. It is also known as imprecision and is determined by calculating the repeat standard deviation of 10 measurements. These errors tend to scatter around the true value and can be minimized by consistent technique and proper training. Systematic Error is the consistent deviation of the measured volume from the selected/true volume, also known as inaccuracy and is determined by establishing the mean value of 10 measurements. Systematic errors can arise from defective or misadjusted devices and need to be corrected through proper calibration and maintenance.

Why are the Z factors listed in our SOP different from factors which can be derived from density reference values (of water)?

The Z factor is included in EN ISO 8655-6 and was incorporated into our SOP. As we understand it, it was incorporated in the standard by the Physikalisch Technische Bundesanstalt in Braunschweig. The Z factor describes the density of water at a particular temperature using a 5th degree polynomial. Furthermore, the density of water is corrected by the density of air (derived from air pressure) and a fixed correction value for balances. These corrections are the key reasons for the deviation from density values in various books of tables.

Can the Multipette M4 and E3/E3x be autoclaved?

No, the Multipette M4 and E3/E3x cannot be autoclaved. The device can be decontaminated using a cloth moistened with 70% isoproanol as disinfectant. Then moisten the cloth with water, wipe down the housing and remove residual cleaning agent. Please ensure that no moisture can get into the device during cleaning.

Why can Multipette M4 and E3/E3x only be operated with Combitips advanced?

When using a positive displacement system, the dispenser and the dispenser tip must be perfectly matched. This will ensure that the high demands with regard to precision and accuracy are reliably met. Combitips advanced are perfectly matched to Eppendorf Multipette M4 and E3/E3x and M4. Combined with the center sleeve of the devices, accurate dosing for dispensing is supported in particular.

What material is the Multipette M4 and E3/E3x made of and what chemicals are they resistant to?

Please refer to the Operating Manuals on our homepage for detailed information on materials. The Multipette M4 has a chemical resistance sheet.

What does the “PhysioCare concept” symbol on the Multipette M4 and E3/E3x mean?

The symbol means that the Multipette M4 and E3/E3x have been developed in accordance with the considerations of the Eppendorf PhysioCare concept. With its three areas, the concept includes the whole range of ergonomics, not just a single element. Instead of focusing on a particular product feature, the products with the PhysioCare symbol provide a holistic solution. Further details on the PhysioCare Concept are available at www.eppendorf.link/physiocare.

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Multipette^® E3/Multipette^® E3x

The Multipette® E3x, a digital repeater pipette by Eppendorf

Multipette® E3/E3x multi-dispensers are ideal to quickly fill plates

The well-organized display of the Multipette® E3/E3x electronic multi-dispenser pipette from Eppendorf

Multipette® E3/E3x multi-dispenser with an assortment of fitting Combitips® advanced positive displacement pipette tips

Pipette Holder 2 with a Multipette® E3x multi-dispenser from Eppendorf

The Pipette Carousel 2 pipette holder from Eppendorf. This pipette accessory holds six Eppendorf pipettes

Multipette® E3x Electronic Multi-Dispenser Held By Scientist (Product Close-Up)

Female scientist using a Multipette® E3x multi-dispenser to quickly fill a microplate

Fill microplates with ease with the Multipette® E3x electronic multi-dispenser from Eppendorf

Easily update the software of your Multipette® E3(x) with the Eppendorf Pipette Software Update Tool

ACT label of sustainability certification for Multipette® E3x pipette

产品信息

无论液体是何性质，Multipette E3/E3x 电动多道分液移液器均能轻松、精准地对其进行分液。此款重复移液器重量轻、易于操作，即使是孔板或系列试管加样也不会造成任何疲劳。搭配使用Combitips^® advanced 分液吸头和ViscoTip^®，便会成为实验室多种液体处理应用的必备工具。

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用途

特征

精确、安全、多功能，长时间移液的理想选择！

在不影响精确度和准确度的情况下，为所有液体类型选择Multipette E3或E3x多道分液器。这些电动正位移分液移液器采用轻量化、人体工程学设计，利用电机驱动实现便捷操作，可有效减轻操作疲劳并降低重复性劳损风险，是实现无压力、长时间重复分液的理想解决方案。Multipette E3与E3x 的分液范围极广，从1 µL至50 mL共5,000种不同分液体积，最小体积增量低至100 nL，同时适配Combitips^® advanced正位移吸头和ViscoTip^®高粘性液专用吸头。Multipette/Combitips 系统具备自动吸头识别功能，适用于各种类型液体，并可防止气溶胶从注射器式吸头中逸出，确保用户可以安全处理有害液体和挥发性溶液。

Eppendorf 1978年首创了Multipette/Combitips多道分液系统，此后对其进行了多次改进。这是首款能够实现一次吸液多次分液的移液系统。其设计理念仍是提升液体处理效率的核心驱动力，并已成为众多涉及孔板或系列试管操作的实验室的标配。2023年，EppendorfMultipette E3与E3x多道分液器与Combitips advanced 分液器吸头共同荣获ACT^®环境影响因子标签，彰显了我们对绿色制造的承诺，以及协助客户做出更环保购买决策的决心。

选择比E3拥有更多操作模式的Multipette E3x

虽然Multipette E3E3已经拥有三种主要操作（模式分液、自动分液和移液），可以满足了许多应用需求，但是Multipette E3xE3x还提供了四种额外模式：正向分液、吸液、吸液/分液、滴定。两款自动重复移液器均搭配使用Combitips advanced分液吸头，确保液体处理便捷、高效、无污染。
Multipette E3x重复移液器还新增吸液/分液模式，可先吸取任意体积液体，然后再将相同体积液体均等分配。该模式为蛋白质生物化学、分子生物学、细胞生物学和微生物学等多个领域应用增添了更多可能性：其一般应用包括提取上清液再分配至多个反应管中。该功能同样有助于判断供液瓶中剩余液体是否足够进行一次试验。更多信息详见第25期白皮书。.

使用Multipette/Combitips 系统有助提高移液精度、缩短移液时间

Combitips advanced吸头轻松连接到Multipette E3/E3x电动多道分液器，在更短时间内完成精确分液。内置传感器可以识别吸头尺寸，并且体积会自动显示在显示屏上，从而消除耗时的体积计算和不正确的分液体积。Combitips advanced 有九种尺寸可供选择，提供更广泛的分液范围，包括最多112种不同容量，仅需一次吸液就能完成更多连续分液操作。
吸头内置活塞可确保移液系统不受液体密度或粘度影响，而且由于不会形成气溶胶，用户可以更加安全地分配含有放射性和/或毒性物质的溶液以及高蒸气压液体。完成操作后，只需按下按钮即可弹出吸头，并在使用后进行处理——始终无污染！

采用正位移设计理念，能够准确移取高粘或挥发性液体

Multipette /Combitips 系统是准确分配粘性液体的理想选择。Multipette E3/E3x电动多道分液移液器能够轻松完成孔板或多排试管加样，同时显著提高移液准确度和精度，搭配使用Combitips advanced分液吸头或专用ViscoTip ，更可处理99.5 %甘油等高粘性液体。

Eppendorf Multipette E3分液器如何使用？

Multipette E3与E3x电动连续移液器配备整洁的用户友好型界面，操作直观便捷。具备自动吸头识别功能，图形化用户界面能够清晰显示所有相关移液参数，防止出现人为错误。“快速入门指南”与“教学视频”能够帮助新用户迅速熟悉移液器各项操作。
使用Eppendorf移液器软件升级工具，您可以轻松升级电动Multipette，并随时了解最新的软件更新和功能！

处置生命周期结束后的包装和分液器。

Multipette E3/E3x分液器包装含有纸板、塑料薄膜等不同材料。我们的 Multipette包装用纸板含有约 92 %的回收材料。请收集包装材料并分类放于贵司废物收集容器，以实际行动支持回收有价值原材料的全球可持续性倡议。包装膜采用低密度聚乙烯（LD-PE）制成，建议选择一家专门从事聚乙烯材料回收的合作伙伴。

Eppendorf 液体处理仪器的设计年限很长，如需处置Multipette ，务必注意其可能已被污染，且包含多个电子元件和一块充电电池。请联系您所在地的生物安全官员和/或废品管理机构，了解电子废物和实验室废物的处理要求及合适做法。如有文件要求，您可以将《Eppendorf消毒证书》作为指导。

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规格	Multipette^® E3x 搭配充电器	Multipette^® E3 搭配充电器	Multipette^® E3x	Multipette^® E3

ACT 标签	是	是	是	是
产品类型	分液器	分液器	分液器	分液器
移液类型	外置活塞系统	外置活塞系统	外置活塞系统	外置活塞系统
通道数量	1-通道	1-通道	1-通道	1-通道
体积范围	1 µL – 50 mL	1 µL – 50 mL	1 µL – 50 mL	1 µL – 50 mL
操作模式	电动	电动	电动	电动
随机测量误差	see Combitips advanced	see Combitips advanced	see Combitips advanced	see Combitips advanced
系统测量误差	see Combitips advanced	see Combitips advanced	see Combitips advanced	see Combitips advanced
显示语言	9 种不同的语言	9 种不同的语言	9 种不同的语言	9 种不同的语言
高温高压灭菌	否	否	否	否
电源	100 – 240 V ±10 %, 50 – 60 Hz	100 – 240 V ±10 %, 50 – 60 Hz	100 – 240 V ±10 %, 50 – 60 Hz	100 – 240 V ±10 %, 50 – 60 Hz
电池类型	可充电锂离子电池	可充电锂离子电池	可充电锂离子电池	可充电锂离子电池
Consumables	Combitips^® advanced，ViscoTip吸头^®	Combitips^® advanced，ViscoTip吸头^®	Combitips^® advanced，ViscoTip吸头^®	Combitips^® advanced，ViscoTip吸头^®
开放系统（第三方耗材）	否	否	否	否
交付内容	多功能分液器，带插头适配器电源，操作手册，简版说明书，Combitips^® advanced混合装（每种尺寸吸头各1个），ViscoTip吸头，Charger Stand 2充电支架	多功能分液器，带插头适配器电源，操作手册，简版说明书，Combitips^® advanced混合装（每种尺寸吸头各1个），ViscoTip吸头，Charger Stand 2充电支架	多功能分液器，带插头适配器电源，操作手册，简版说明书，Combitips^® advanced混合装（每种尺寸吸头各1个），ViscoTip吸头	多功能分液器，带插头适配器电源，操作手册，简版说明书，Combitips^® advanced混合装（每种尺寸吸头各1个），ViscoTip吸头