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- Challenges and Chances: A Review of the 1st Stem Cell Community Day
- Summertime, and the Livin’ Is Easy…
- Follow-on-Biologics – More than Simple Generics
- Bacteria Versus Body Cells: A 1:1 Tie
- Behind the Crime Scene: How Biological Traces Can Help to Convict Offenders
- Every 3 Seconds Someone in the World Is Affected by Alzheimer's
- HIV – It’s Still Not Under Control…
- How Many Will Be Convicted This Time?
- Malaria – the Battle is Not Lost
- Physicians on Standby: The Annual Flu Season Can Be Serious
- At the Forefront in Fighting Cancer
- Molecular Motors: Think Small and yet Smaller Again…
- Liquid Biopsy: Novel Methods May Ease Cancer Detection and Therapy
- They Are Invisible, Sneaky and Disgusting – But Today It’s Their Special Day!
- How Many Cells Are in Your Body? Probably More Than You Think!
- What You Need to Know about Antibiotic Resistance – Findings, Facts and Good Intentions
- Why Do Old Men Have Big Ears?
- The Condemned Live Longer: A Potential Paradigm Shift in Genetics
- From Research to Commerce
- Chronobiology – How the Cold Seasons Influence Our Biorhythms
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Still Bagging Samples?
Lab Academy
The storage style of samples in the ULT freezer can result in a lively discussion in lab teams. Freelance storage with hundreds of single microtubes in a plastic bag or even spread in one ULT freezer compartment can result in tough discussion within the lab team.
Different sizes
For optimal and economical usage of the existing storage room at -80°C, vessels should be stored in freezer boxes which fit exactly to the vessel dimensions. For sure, 2 mL vessels can be stored in boxes dedicated for 50 mL conical tubes. But this style is uneconomic as you waste a lot of storage space. Besides, removing microtubes out of the 120 mm deep boxes is quite uncomfortable. Nowadays, there are all kind of different box sizes available, fitting to all commonly used vessel types. Different inner grid variants provide a perfect fit to these formats: cryogenic tubes, microtubes, conical tubes 15 and 50 mL, and other laboratory vessels. Common size standard is “inch”, e.g. 2 in boxes for 53 mm microtubes. The footprint of the freezer boxes is by standard 133 mm x 133 mm.Related metal racks are standardized for these footprints.
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inch | mm | Example tubes |
2 | 53 | 1.5/ 2.0 mL Microtubes |
2.5 | 64 | 5 mL microtubes |
3 | 76 | 3 mL cryotubes |
4 | 102 | 5 mL cryotubes |
5 | 127 | 15/ 50 mL conical tubes |
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Material
In principle, there are just two materials used for freezer boxes: Either cardboard or plastic material. The cardboard material (mostly white surface) are cost-efficient but have a limited lifetime due to the humidity and even wetness during daily routine working at the freezer. As the cardboard material can be recycled, the sustainable factor is high.Lire plus
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In contrast to that, plastic boxes have a higher price but the boxes easily survive many years of usage due to their robustness. Besides, differently colored boxes support intuitive sample sorting (e.g. blue box for sample type A or user 1 versus orange boxes for sample type B or user 2).
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Structure
There is a temperature delta of more than 100°C between the ambient temperature in the lab and the frozen samples. The thermodynamic tension on the material requires reinforcement of the boxes by plastic bracing as well as ventilation ports for larger freezer boxes as for 15/ 50 mL tubes conical for an even temperature adaption.Lire plus
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Labeling
The labeling of boxes can be done directly on the surface, via adhesive labels, or via paper slip attached by transparent duct tape. Independent from handwritten, printed labels, or barcodes, a security layer of transparent duct tape might help against mechanical damage.Printed labels (plain writing or barcode) are recommended to make reading as easy as possible for everyone else besides the author.
Number of samples
At the beginning of a research life, the number of samples resp. vessels is limited. The approach “all protein samples belong to the first box, the DNA samples belong to the second box, ...” fits. Over the time, the accumulation of samples exceeds the capacity of this sorting style.A sorting system based on metal racks provides a systematic approach. Sample management for easy refinding of samples can be handled by dedicated databases.
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