Eppendorf do BrasilAplicaçõesPharmaCentrifuge SolutionsHigh-Throughput Screening Solution

High-Throughput Screening Solution

Centrifuge Solution for high-throughput screening

High-throughput screening for the identification of antibody hits in culture supernatants of hybridoma clones is usually performed in microtiter plates (MTPs) with 96 or more wells. Various methods are required and all include multiple centrifugation steps of temperature-sensitive samples in MTPs. Refrigerated benchtop Centrifuge 5920 R in combination with swing-bucket Rotor S-4x1000 with plate/tube buckets offer super-high capacity of up to 28 MTPs per run and an advanced cooling performance for optimal sample protection.

Increased Efficiency

  • Super-high capacity of 28 x MTPs per run ideal for high-throughput applications.
  • Fast loading and unloading with high-capacity plate carrier for 7 MTPs.
  • In addition to the FastTemp function for super fast pre-cooling, FastTemp pro® allows for automated pre-cooling based on pre-programmable time and date. Simply turn the centrifuge into standby mode when you leave your lab and let FastTemp pro take care of pre-cooling in the morning so you don’t have to.

Improved Sample Safety

  • End of run counter (time after rotor stopped) to provide information whether PBMCs are still well separated by Ficoll-gradient and thus a maximum yield of viable cells is obtained.
  • Accurate cooling with intelligent Dynamic Compressor Control (DCC) for optimal sample integrity. Unlike other compressors which switch back and forth between 0% and 100% performance output, DCC allows for a constant and smooth temperature regulation. This results in a precise cooling performance and the set temperature is kept stable at all time.
  • In the case of a plate crack you can carry the closed bucket securely to the biosafety cabinet with optional aerosol-tight caps.

 

 

Better Ergonomics

  • New optional Eppendorf mobile tables lower centrifuge to an ergonomic height for easy loading and unloading of samples and rotors for a more ergonomic work environment.
  • Soft-touch lid closure system for ergonomic lid operation. There is no need to exert unnecessary force when closing the centrifuge lid - you can close the centrifuge with ease.
  • Quiet operation to improve your work environment.

 

Components

Centrifuge 5920 R
  • High-throughput multipurpose centrifuge with very high capacity, refrigerated for temperature sensitive samples
  • New optional mobile tables lower centrifuge to an ergonomic height for easy loading and unloading
  • Max. capacity: 4 × 1,000 mL/4 × 7 MTP
Plate Carrier
  • For MTP, DWP and PCR plates
  • Max. capacity: 7 x MTP/PCR plates or 2 x DWP
  • Max. speed: 3,030 × g
Rotor S-4x1000 with plate/tube buckets
  • High capacity swing-bucket rotor for high-throughput plate and tubes applications
  • Aerosol-tight caps optional available
  • Max. speed: 3,076 × g (3,700 rpm)

Recommended Consumables for Key Methods

Recommended Consumables

Key Method Consumables Advantages

High-throughput screening

  
  • DWP 96x500µl Protein LoBind®
  • DWP 384x200µl White, Protein LoBind®  
  • Microplate 384 V-PP white/clear, Protein LoBind®
  • Microplate 96 F-PP white/clear, PCR-Clean   
  • Eppendorf twin.tec® PCR Plate 384, clear
 		  
  • Minimization of rest volume and increased mixing & resuspension efficiency (even in 384 format) with optimized well geometry RecoverMax®
  • Reduced  intra- and inter-lot variance due to the sturdy construction of plates and the high lot-2-lot consistency
  • Minimization of unspecific sample loss with LoBind® consumables
 
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