Visit our support center

If you have any questions or would like to contact us, please visit the Support Center.

Pipetting knowledge hub

NGS

Next Generation Sequencing (NGS) enables comprehensive analysis of genetic material and is reshaping genomics research and diagnostics.

About the NGS workflow

Library Preparation for Next Generation Sequencing (NGS) involve readying available DNA or RNA samples for sequencing. Depending on the research goal, e.g. whole genome or whole exome sequencing, workflows vary in detail but follow the same general principal. This includes normalization, fragmentation, and adapter ligation. Samples then undergo size-selection and are finally amplified. Lastly, quality control is being done before loading the sequencer's flow cell.
However, what all NGS library prep workflows have in common is the need for hundreds of precise pipetting tasks, be it for reagent addition, bead handling, or normalization steps. All the time while using costly reagents and having strict requirements towards reproducibility.

Challenges in NGS

Tedious, costly workflows that require high focus over hours
Risk of cross-contamination and sample mix-up
Demand for consistent results and traceability
Handling precious samples, low volumes, and challenging liquids

How our automated liquid handlers help

epMotion automates the lion's share NGS Library Prep workflows, from reagent addition to magnetic bead handling and normalization. We offer pre-validated protocols for all important NGS vendors and their most-demanded kits, so users can automate their library prep efficiently and safely. Our flexible software enables users to optimise protocols to adapt to their needs, while tracability is achieved by built-in documentation and tracking features. In terms of throughput, most protocols are amenable to batchsizes of up to 96.

Discover our epMotion systems

Why our expertise matters

We understand that your sequencing results depend on the quality of your library prep. You'll receive in any step regarding automation of NGS library prep protocols by our application specialists, while our instrument ensures consistent, high-quality libraries with minimal risk of pipetting errors. Due to our long-standing collaborations with major NGS vendors, we can join forces and facilitate finding the solution that suits you best. With Eppendorf, you gain a partner who knows the critical points of NGS Library Prep and how to solve them.

Explore a wide variety of ready-to-use protocols for NGS and more. Discover how easy it is to own your solution

Visit our methods overview

Access all the resources you need in one place with the Eppendorf
download center. Find manuals, certificates, brochures, and more
to support your laboratory work.

Explore our downloads

Está a punto de abandonar este sitio.