Exosomes are naturally occurring extracellular vesicles secreted by cells with the primary function of carrying molecular payloads such as proteins or genetic material between cells. In doing so, exosomes have the ability to modulate cell communication, immune regulatory processes, tumor metabolism, and regenerative/degenerative processes. They have recently become of great interest for their use as therapeutic and diagnostic tools. Research into exosomes in the preclinical cell and gene therapy (CGT) space is growing rapidly, creating an ever-greater need for large-scale production to meet the demands of exosome research and development projects. The use of stirred-tank bioreactors represents one of the best approaches to ensuring optimal production conditions and enabling tight monitoring and control of the bioprocess in a highly scalable setup. The use of centrifuges is considered the gold standard for the purification of exosomes from the culture medium.