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Next Generation Sequencing

NGS library preparation

DNA sequencing is a method to decipher the base sequence in nucleic acids. The elucidation of the DNA sequence is essential for the understanding of virtually all biological processes e.g. human disease biology, inheritance, immunology, oncology, cellular biology. First generation sequencing devices use the capillary electrophoresis-based Sanger sequencing technique. The invention of the Next Generation Sequencing (NGS) technique massively improved the weaknesses of the 1st generation sequencing technique e.g. low throughput, scalability, speed, and resolution. NGS allows massive parallel sequencing which reduces the costs and increases the speed of DNA sequencing.

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Automated NGS Solutions

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Streamlined

Small Upgrade, Huge Improvement. Get The Most Out Of Your NGS Library Preparation with the epMotion®.

Eppendorf twin.tec® PCR Plates 96 LoBind Increase Yield of Transcript Species and Number of Reads of NGS Libraries

Sequencing is an increasingly important technique in life sciences. Multiple sequencing strategies exist, but most of them require high sample quality and, often, a large amount of starting material.

Validation of a next generation sequencing based HLA Typing system

The Illumina TruSight HLAv2 kit for typing of HLA-A, -B, -C, -DRB, -DQ and -DP for solid organ and haematopoietic stem cell transplantation have been validated and implemented. In addition we have validated the use of this kit on the Eppendorf epMotion® 5075 liquid handling robot.

Improve your laboratory workflow

Next-generation sequencing (NGS) requires optimal, thorough sample preparation upstream of the sequencing process to ensure the best possible results