How many parameters can I control using a DASGIP or DASbox system?

DASGIP Parallel Bioreactor Systems provide comprehensive concurrent monitoring and control of process parameters. During the process run, events are logged together with up to 70 online process variables per bioreactor. In addition to the various DASGIP modules for monitoring and control, with DASware analyze users can integrate analytical devices provided by third parties. Seamless integration into the DASGIP or DASbox system even allows for closed loop solutions such as automated feeding strategies based on the current glucose concentration. Each process value can be used as a trigger enabling users to automate their process.

How many parameters can be displayed in a process overview in DASware control?

DASware control users can freely select all relevant process variables of all or selected bioreactors to be displayed in one chart. There are no limitations in the number of process parameters. When using DASware analyze, the displayed process parameters even include integrated data from third party laboratory devices, such as nutrient analyzers, mass spectrometers or Raman. The software features tables and trend graphs of all measured values. It continuously logs and stores all data and events. These data are immediately accessible for evaluation using e.g. Microsoft Excel. Advanced charting functionalities ease further data analysis and comparison of runs.

Do I need programming skills to automate my process in DASware control?

DASware control includes a set of easy to implement automation features without any need of programming. Profiles and triggers allow for various control strategies, all of them easy to select by mouse click with parameters entered manually or just copy-pasted. Users with programming skills and special control requirements, however, are free to run scripted control strategies and profiles as well.

Can I change triggers, profiles, cascades and scripts online in DASware control?

Set-points, profiles, cascades, and scripts are online editable in DASware control. Offline values and events can be entered during or after the run and documented with a time stamp. These time stamps can be set individually, e.g to meet the time, when the sample was taken. This provides users with great flexibility to interact with the control system and reconsider parameter settings even in highly automated processes. All events, including online changes, are recorded in a SQL Server database.

How difficult is it to modify a DASGIP system subsequently?

Due to the unique modular design of the DASGIP Parallel Bioreactor System upgrades and modifications can easily be done: Functions like OD monitoring, exhaust analysis or extra feed lines can be added as modules to the control system. It is just as easy to substitute the bioreactor type or to upgrade a 4-vessel system to eight vessels. Actually, system modifications are that simple, some customers even share specific modules. For instance, the DASGIP GA4 exhaust analyzer is renowned for being moved from one bioreactor system to another. Like this, it delivers reliable in-depth analysis of OTR, CTR, and RQ for many bioreactors on demand.

How many speed-controlled pumps do the DASGIP bioreactors feature?

Up to eight peristaltic speed-controlled pumps per vessel offer continuous flow rates from 0.3 mL/h - 5 L/h. Each pump can be operated independently. Two different pump head sizes for the multi pump modules DASGIP MP4 and MP8, fitting various tube sizes make the DASGIP multi pumps a flexible and accurate solution.

How are DASGIP vessels agitated?

DASGIP bioreactors feature powerful overhead drives with agitation speeds ranging 30 – 1,600 rpm. The DASGIP TC4SC4 controls temperature and agitation in up to four bioreactors. Users can individually define each outlet set-point as well as choose from different operation modes (e.g. continuous or interval agitation).

Can I integrate DASGIP with my existing historians & supervisory control?

Yes, with the software DASware connect an optional server allows corporate-wide exchange of all process data via seamless integration with historians and supervisory control systems such as Delta V, OSI Pi, etc. This includes events, set-up information, and system status.

How can I harness data generated by the DASGIP system?

With DASware control all data and events are logged in one central SQL Server database. Further features such as export into Excel, PDF, and other file formats via one mouse click and strong charting capabilities ease plotting and data analysis. To harness data from numerous projects, on the long-run advanced archiving capabilities and data retrieval is required. DASware discover offers comprehensive and user-defined archiving functions and data retrieval for powerful data comparison and analysis. Users can, for example, link the utilized strain or cell line with its media composition, the controller setpoints and feeding profiles as well as with achieved product yields or viable cell densities to select for the best optimized combination and process.

Does DASware control give any alarms in case of critical deviation?

Critical deviation of process parameters such as pH, DO, redox, and temperature are color-coded in the software and saved in the event log. DASware control professional additionally allows notification of supervisory control systems or mobile communication tools via SMS or e-mail.

Can you help adjusting my controller loop parameters in DASware control?

DASGIP controllers are PI controllers, which are configured with default controller parameters adapted to the type of vessel and default experimental requirements. Depending on special customer requirements it might be necessary to modify these parameters. Our service will be happy to help you finding the right settings.

How to calibrate a pH sensor in DASware control?

DASware control software allows for parallel calibration of all sensors used within one system with all data logged in the central database together with set-points, profiles, cascades, process values, user interactions and other events. Good maintenance will prolong the sensors' lifetime whereas high temperatures (i.e. during autoclavation), alkaline solutions (i.e. during alkaline deactivation), repeated etchings and improper maintenance will reduce the electrode's lifetime.

How reliable are exhaust data when working at benchtop scale?

Exhaust oxygen and carbon dioxide concentrations of multiple bioreactor units are continuously monitored with the DASGIP GA exhaust analyzer. Together with the mass flow measurement and the input values of oxygen and carbon dioxide concentrations, automatic calculation of oxygen transfer rate (OTR), carbon dioxide transfer rate (CTR), and respiratory quotient (RQ) is possible, permitting direct conclusions on the metabolic state of a culture. As the DASGIP GA considers humidity, volume, and total flow typical sources for inaccurate OTR and CTR data in small working volumes are eliminated.

Is it possible to automatically control the foam in DASGIP vessels?

All DASGIP vessels can be equipped with foam control components. All you need is a level/foam sensor connected to the DASGIP sensor module (e.g. PH4PO4L) and a feed line for dosing of the antifoam agent. Now foam formation can be detected by the level sensor and antifoam agent is automatically pumped into the vessel until all foam is collapsed. The DASware control software allows any feed line to be used for antifoam or level control. Since medium composition has a strong impact on the electrical conductivity of medium and foam, every conductivity-based level sensor needs to be adjusted to foam and medium conductivity. DASware control supports monitoring conductivity and free adjustment of the conductivity threshold.

What maintenance is required for Eppendorf bioprocess systems?

As all complex technical systems Eppendorf bioprocess equipment should be maintained regularly to keep all parts in good condition. This maintenance avoids cost-intensive down times and contributes to preservation of value. A complete preventive maintenance is recommended once a year. Additional maintenance actions are recommended to be executed by the user prior to every run or in regular cycles (e.g. every month). Our service will be happy to advice.

What do I have to consider when returning equipment for repair?

Equipment returned to Eppendorf for service or any other reason needs to be decontaminated first. Please fill out the Decontamination Form that you will find in the “Service & Support” section of the Eppendorf website and attach it to the package. Equipment which was sent without the completely filled form cannot be handled. This form must also be completed before any on-site service, whether covered under warranty or not, can commence.

What does your warranty cover?

Eppendorf issues a warranty of one year from the day of delivery on its products in accordance with our terms and conditions. Excluded are consumables such as cables, tubing, glassware, and sensors as well as used material.

Does Eppendorf offer bioreactor training?

We highly recommend a training at the customer’s site for all new customers. Besides this initial training, Eppendorf also offers individual trainings adapted to the user’s requirements like training of new employees or advanced training/recapitulation for employees already having experience with DASGIP or DASbox systems. All trainings are performed at the customer’s site using the customer’s equipment to have the training focused on the customer’s demands. The structure and methods of the training can be chosen to the customer’s requirements from general overview to detailed sessions on specific products or issues. Training in small groups is as possible as personal guidance.

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DASGIP^® Parallel Bioreactor System

Informazioni sul prodotto

Il DASGIP Parallel Bioreactor System è un sistema di bioreattori per la ricerca e lo sviluppo dei processi con volumi di lavoro da 200 mL a 1,8 L. Può gestire in paralleo fino a 16 giare in vetro o BioBLU^® Single-Use Bioreactors ed è adatto per la fermentazione microbica e la coltura cellulare, compresa la coltivazione di cellule staminali. Il design modulare consente agli utenti di scegliere la configurazione più adatta alle proprie esigenze applicative.

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Informazioni sul prodotto

Dati tecnici

Applicazioni

Caratteristiche

Il DASGIP Parallel Bioreactor System consente di sviluppare processi altamente efficienti grazie a un controllo preciso dei parametri di processo critici, a un rapido confronto di numerose condizioni sperimentali e a un utilizzo ottimale di tutti i dati prodotti.
• Il funzionamento in parallelo di massimo 16 bioreattori consente di risparmiare tempo e spazio e migliora la riproducibilità.
• I bioreattori sono facilmente convertibili da monouso a riutilizzabili e viceversa.
• La suite software DASware^® consente un monitoraggio e un controllo precisi dei parametri di processo critici, nonché l'implementazione di loop di feedback personalizzati, strategie di alimentazione e altre funzioni.
• L'integrazione perfetta con il Bioprocess Autosampler consente un campionamento automatico 24 ore su 24, 7 giorni su 7, per ottenere set di dati completi.
• Si collega al software BioNsight^® cloud per il monitoraggio remoto dei processi e un'analisi semplice e remota di tutti i set di dati per prendere decisioni informate.

Utilizzo di bioreattori multipli in parallelo

• Il DASGIP Parallel Bioreactor System è concepito come un sistema a 4 moduli con fino a 16 bioreattori in funzione in parallelo.
• I valori richiesti, le strategie di controllo e il tempo di inoculazione di ciascun bioreattore possono essere controllati individualmente da un unico sistema software. In questo modo è possibile testare più condizioni sperimentali in parallelo.

Automazione del campionamento nei bioprocessi

• Il sistema del bioreattore può essere perfettamente integrato con il Bioprocess Autosampler, che preleva campioni da più bioreattori 24 ore su 24, 7 giorni su 7, a intervalli brevi e regolari, e li conserva a temperatura controllata per analisi successive.
• Il Bioprocess Autosampler può essere installato con sistemi di controllo dei bioreattori nuovi oppure già esistenti in un secondo momento.

Adatto per colture cellulari e fermentazioni microbiche

• Il DASGIP Parallel Bioreactor System è adatto per affrontare le esigenze poste dal trattamento di diversi tipi di cellule, tra cui batteri, lieviti, cellule di insetti, cellule di mammiferi differenziate e cellule staminali.
• Due varianti sono state studiate per supportare i requisiti di flusso di gas delle applicazioni microbiche e delle colture cellulari.
• Il DASGIP Parallel Bioreactor System è compatibile con i bioreattori progettati per soddisfare le esigenze delle colture microbiche e cellulari.

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Specifiche	DASGIP^® Parallel Bioreactor System	DASGIP^® Parallel Bioreactor System

Applicazione	Coltura cellulare	Microbiologia
Numero di unità in parallelo	Fino a 16	Fino a 16
Software	DASware control, altro DASware in opzione	DASware control, altro DASware in opzione
Interfaccia utente	Computer di processo con monitor	Computer di processo con monitor
Consumo di energia tipico (sistema a 4 elementi senza computer di processo)	480 W (a 230 V)/ 432 W (a 115 V) (595 W/552 W senza DASGIP Bioblock)	541 W (a 230 V)/ 467 W (a 115 V) (639 W/584 W senza DASGIP Bioblock)
Dimensioni (L × P × H, sistema a 4 elementi senza computer di processo)	140 x 70 x 53 cm (55 x 28 x 21 in)	140 x 70 x 53 cm (55 x 28 x 21 in)
Peso tipico	78 kg (60 kg senza DASGIP Bioblock)	96 kg (78 kg senza DASGIP Bioblock)
Batteri/Lieviti/Funghi	sì	sì
Mammiferi/Animali	sì	sì
Insetti	sì	sì
Giare intercambiabili	sì	sì
Validabile	no	no
Cellule staminali	sì	sì

Bioreattori
Recipienti	Giare in vetro / monouso	Giare in vetro / monouso
Sterilizzazione	Autoclavabile/ presterilizzato	Autoclavabile/ presterilizzato
Volume totale	da 1,5 L a 2,3 L	da 1,3 L a 2,1 L

Agitazione
Azionamento	Azionamento di testa a comando diretto/a comando magnetico	Azionamento di testa a comando diretto/a comando magnetico
Intervalli di velocità	301,250 –  rpm (standard)/ 1001,600 –  rpm (opzionale)	1001,600 –  rpm (standard)/ 301,250 –  rpm (opzionale)
Giranti	A pale inclinate	Tipo Rushton

Gassificazione
Alimentazione di gas	TMFC; overlay e/o diffusore	TMFC/rotametro; diffusore
Portate gas standard	0,1 – 50 sL/h, 0,1 – 40 sL/h CO₂ (DASGIP MX4/4, altri moduli disponibili)	0,5 – 250 sL/h, 0,5 – 150 sL/h CO₂ (DASGIP MX4/4H, altri moduli disponibili)
Miscelazione di gas standard	Air, N₂, O₂ e/o CO₂	Air, N₂, O₂ e/o CO₂

Alimentazione
Linee di alimentazione per giara	Fino a 8	Fino a 8
Portate di alimentazione standard (a seconda del diametro del tubo)	da 0,3 – 9,5 mL/h a 13 – 420 mL/h (DASGIP MP8) da 10 – 70 mL/h a 0,4 – 5 L/h (DASGIP MP4)	da 0,3 – 9,5 mL/h a 13 – 420 mL/h (DASGIP MP8) da 10 – 70 mL/h a 0,4 – 5 L/h (DASGIP MP4)

Monitoraggio e controllo
Regolazione della temperatura	Riscaldamento e raffreddamento integrati in DASGIP Bioblock (dita fredde aggiuntive opzionali)	Riscaldamento e raffreddamento integrati in DASGIP Bioblock (dita fredde aggiuntive opzionali)
Intervallo di temperatura standard (a 25° C, temp. ambiente)	5 K al di sopra della temperatura dell'agente di raffreddamento – 99°C (DASGIP Bioblock)¹⁾	5 K al di sopra della temperatura dell'agente di raffreddamento – 99°C (DASGIP Bioblock)¹⁾
Controllo del pH	−	Acido e/o base e altre configurazioni
Controllo OD	Cicli in cascata (concentrazione di O₂, portata del flusso di gas) e altre configurazioni	Cascata (velocità di agitazione, concentrazione di O₂ opzionale, portata di gas opzionale) e altre configurazioni
Misurazione ORP (redox)	-	Opzionale
Livello/schiuma	Opzionale	Opzionale
Misurazione densità ottica	Opzionale (DASGIP OD4)	Opzionale (DASGIP OD4)
Condensazione di scarico	Raffreddamento ad acqua o senza liquidi (Peltier con DASGIP EGC4)	Raffreddamento ad acqua o senza liquidi (Peltier con DASGIP EGC4)
Analisi dello scarico	Opzionale (DASGIP GA4)	Opzionale (DASGIP GA4)
1)Si applicano le condizioni di utilizzo specifiche dell'applicazione.