For which SmartBlocks can the ThermoTop be used?

The ThermoTop is compatible with folowing SmartBlocks: SmartBlock 0.5 mL SmartBlock 1.5 mLSmartBlock 2.0 mL SmartBlock plates SmartBlock PCR 96 SmartBlock PCR 384 SmartBlock DWP 500 SmartBlock DWP 1000

What is the temperature control range of the ThermoTop?

The temperture control range of the ThermoTop corresponds to the temperature control range of the device or Smartblock with which the ThermoTop is used. Further information can be found in the operating manual.

Can cell culture plates be used in the SmartBlock plates?

Cell culture plates comply with the SBS standard can be used in the SmartBlock plates. Temper polystyrene plates up to 70°C max. Further information can be found in the operating manual.

Is it possible to put the SmartBlocks for a rapid cool down into a -20°C freezer?

The SmartBlocks can be used at ambient temperatures of 5°C – 40°C and can be cooled appropriate. Cooling down of SmartBlocks in a freezer should not be done.

Which SmartBlocks and vessels can be used with ThermoMixer F models?

The ThermoMixer F models possess a fix installed SmartBlock. ThermoMixer F0.5: installed SmartBlock 0.5 mL for 24 0.5 mL mirco test tubes ThermoMixer F1.5: installed Smartblock 1.5 mL for 24 1.5 mL micro test tubes ThermoMixer F2.0: installed SmartBlock 2.0 mL for 24 2.0 mL mirco test tubes ThermoMixer FP: installed SmartBlock plates for all common plates formats Detailed informations can be found in the operating manual.

Do the IsoRacks fit upon the SmartBlocks for microreaction tubes (0.5 mL; 1.5 mL; 2.0 mL)?

No. The IsoRacks fit only upon the Thermoblocks for the older devices Thermomixer comfort, Thermomixer compact & ThermoStat plus.

Can PCR plates be used in SmartBlock plates respectively ThermoMixer FP?

SmartBlock plates and ThermoMixer FP (with the fixed SmartBlock plates) are dedicated for using MTP’s, DWP’s and also other skirted plates which meet the SBS standard. For optimal usage of PCR plates the SmartBlock PCR 96 and PCR 384 in combination with ThermoMixer C or ThermoStat C is the proper choice. These SmartBlocks possess conical bores which surround the conical wells of PCR plates properly for a perfect temperature transition.

SmartBlock cryo thaw on ThermoMixer C - What is the ‘Thawing cells’ program?

The 'Thawing cells' program is an optimized program to thaw mammalian cells in 1.8-2.0 mL cryovials: reproducibly contamination free standardized It is a ready-to use program, in most cases no changes are required. The pre-set time is 3 minutes (can be extended up to 5 minutes), the pre-set mixing frequency is 500 rpm (can be adapted between 300 – 800 rpm), the temperature profile is fixed. The settings are based on tests with standard mammalian cell lines (CHO, HEK, S16) up to highly sensitive stem cells (hiPSCs) at tested conditions with 2.0 mL cryovials / 1.0 mL fill (1 – 5 vials in parallel). Other volumes or more vials may require an adaption of thawing time. Further information can be found in the Application Note 437 .

What is the difference between the SmartBlock cryo thaw and the SmartBlock cryo?

The 'SmartBlock cryo thaw' replaces the 'SmartBlock cryo'. Newly implemented is an automatic function for thawing cells (program 'Thawing cells') which is available in combination with the ThermoMixer C.

Is it possible to use the SmartBlock cryo thaw with the ThermoStat C?

The SmartBlock cryo thaw is still compatible with the ThermoStat C. However, the automatic thawing function 'thawing cells' is limited to the ThermoMixer C as it requires mixing.

The ThermoMixer C does not show the program ‘Thawing cells’ when inserting the SmarBlock cry thaw. Why?

The software version 3.5.0 or higher is required on the ThermoMixer C to operate the program 'Thawing cells'. How to upgrade the software is described in the operating manual.

Is it possible to use the SmartExtender with the SmartBlock cryo thaw?

The Smart Extender can be used with the SmartBlock cryo thaw, but not in parallel a 'Thawing cells' program is running. In case the user is operating the SmartExtender and intends to start the thawing procedure, the user is informed by a message with the option to stop the SmartExtender and vice versa. There is no automatic stop or re-start of the running program to not disturb the application.

SmartBlock cryo thaw - Does the mixing during the thawing program harm the cells?

No, on the contrary, a slight mixing (500 rpm combined with a small 3 mm orbit) helps to distribute the temperature in the vial evenly and reduces temperature gradients and inhomogeneous warming of the cells by preventing sedimentation. The reproducibility and suitability of the thawing method have been demonstrated by: – in‑vial temperature measurements – analysis of post‑thaw cell viability – further downstream tests (confirming pluripotency for stem cells). Further information can be found in the Application Note 437 .

Is it possible to use former SmartBlock cryo with the program ‘thawing cells’?

No, the program 'thawing cells' is limited to the SmartBlock cryo thaw.

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Eppendorf SmartBlock

Eppendorf ThermoStat C con selezione di Smartblocks differenti

Rimozione di Eppendorf SmartBlock esercitando pressione sulla leva (passaggio 1)

Rimozione di Eppendorf SmartBlock esercitando pressione sulla leva (passaggio 2)

Rimozione di Eppendorf SmartBlock esercitando pressione sulla leva (passaggio 3)

Eppendorf SmartBlock per 24 provette da 1,5 mL

Eppendorf SmartBlock per 24 provette da 2,0 mL

Eppendorf SmartBlock for 8 x 5.0 mL vessels

Eppendorf SmartBlock per 8 provette da 15,0 mL

Eppendorf SmartBlock per 4 provette da 50,0 mL

Adattatore per provette coniche da 25 mL da utilizzare in Eppendorf ThermoMixer C

Eppendorf SmartBlock per provette 24 x 12 mm

Eppendorf SmartBlock for 24 x cryo vessels with several cryovials

Eppendorf SmartBlock DWP 500 with white DWP

Eppendorf SmartBlock DWP 1000 con DWP da banco

Eppendorf SmartBlock for plates incl. piastre DWP bianco-blu

Image – Eppendorf SmartBlock for 96-well PCR plates con piastra PCR semi-skirted blu da 96 pozzetti

Eppendorf SmartBlock for 384-well PCR plates con piastra PCR rossa

Eppendorf Transfer_Rack_1.5/2.0 mL_per trasferire i campioni da Eppendorf ThermoMixer C

Image – Set of Eppendorf SmartBlocks with ThermoTop

Informazioni sul prodotto

Sostituibili in una manciata di secondi.
Questi SmartBlocks, controllati individualmente da sensori, sono la soluzione perfetta per voi per mantenere temperature uniformi e accurate. Un'ampia varietà di blocchi termici per tutti i più comuni recipienti di laboratorio consente l'effettuazione di una vasta serie di applicazioni, venendo utilizzati con Eppendorf ThermoMixer^® C (riscaldamento/raffreddamento e miscelazione) e ThermoStat C (riscaldamento/raffreddamento).

Mostra più informazioni sul prodotto

Troverai ulteriore materiale scaricabilein fondo a questa pagina

Informazioni sul prodotto

Dati tecnici

Caratteristiche

Necessità di utilizzare diverse misure di contenitori all'interno del vostro flusso di laboratorio?
La flessibilità non è mai stata così semplice. Eppendorf offre una vasta gamma di Eppendorf SmartBlocks per provette da 0,2 mL a 50 mL come anche piastre (MTP, DWP e PCR da 96/384 pozzetti) da utilizzare con Eppendorf ThermoMixer^® C e ThermoStat C. Tutti gli Eppendorf SmartBlocks sono dotati dello straordinario sistema Eppendorf QuickRelease che rende la sostituzione dei blocchi estremamente semplice e veloce.
Per un abbinamento e un trasferimento della temperatura ottimali consigliamo di utilizzare Eppendorf Deepwell Plates con il corrispondente Eppendorf SmartBlock DWP 500 e Eppendorf SmartBlock DWP 1000. Considerare che Eppendorf SmartBlock DWP 500 e Eppendorf SmartBlock DWP 1000 possono essere utilizzati solo con Eppendorf Deepwell Plates.

Nessun utensile a portata di mano?

Gli Eppendorf SmartBlock possono essere sostituiti con facilità. Basta premere la leva della parte anteriore del blocco e questo viene sostituito in pochi secondi, senza bisogno di strumenti.

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Specifiche	Eppendorf SmartBlock 0.5 mL	Eppendorf SmartBlock 1.5 mL	Eppendorf SmartBlock 2.0 mL	Eppendorf SmartBlock 5 mL	Eppendorf SmartBlock 15 mL	Eppendorf SmartBlock 50 mL	Eppendorf SmartBlock PCR 96	Eppendorf SmartBlock PCR 384	Eppendorf SmartBlock plates	Eppendorf SmartBlock DWP 500	Eppendorf SmartBlock DWP 1000	Eppendorf SmartBlock cryo thaw	Eppendorf SmartBlock 12 mm

ThermoTop	sì	sì	sì	−	−	−	sì	sì	sì	sì	sì	no	−
Capacità campioni	24 × microprovette (0,5 mL)	24 × microprovette (1,5 mL)	24 × microprovette (2,0 mL)	8 × 5 mL	8 × 15 mL provette coniche	4 × 50 mL provette coniche	1 piastra PCR 96	1 piastra PCR 384	1 × micropiastra per test / 1 × piastra deepwell	Eppendorf Deepwell Plates 96/500 µL	Eppendorf Deepwell Plates 96/1.000 µL	24 × provette cryo (1,5/2,0 mL)	24 × microprovette, diametro 12 mm
SmartExtender	sì	sì	sì	sì	sì	sì	sì	sì	sì	sì	sì	sì	sì
ThermoMixer C / ThermoStat C	sì	sì	sì	sì	sì	sì	sì	sì	sì	sì	sì	sì	sì
Temp. max.	100 °C	100 °C	100 °C	100 °C	100 °C	100 °C	100 °C	100 °C	100 °C	100 °C	100 °C	110 °C¹⁾	110 °C¹⁾
Coperchio	sì	sì	sì	−	−	−	sì	sì	sì	sì	sì	−	−
Max. velocità	2.000 rpm	2.000 rpm	2.000 rpm	1.000 rpm	1.000 rpm	1.000 rpm	2.000 rpm	3.000 rpm²⁾	3.000 rpm³⁾	1.600 rpm	1.600 rpm	2.000 rpm	2.000 rpm