Molecular Biology

In the field of Molecular Biology the goal is to analyze and understand the molecular basis of biological processes. It concerns itself with the function of DNA/RNA sequences and the interaction between DNA, RNA and proteins. Furthermore, this field overlaps to a great extend with other areas of biology and chemistry, particularly biochemistry and genetics. One of the most important molecular biology methods is the polymerase chain reaction (PCR). It is used to exponentially amplify a specific segment of DNA for various downstream applications like DNA cloning (genetic engineering), gene expression analysis (RT-PCR, RT-qPCR), genetic fingerprinting (paternity testing, forensics), sequencing (Next generation sequencing) and others more.



Eppendorf Client Interview Michael Dreifke
Eppendorf Client Interview Michael Dreifke

"A major advantage of the Eppendorf BioSpectrometer is the temperature-regulated cuvette shaft"

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Eppendorf Client Interview Dr. Silvia Ferrara
Eppendorf Client Interview Dr. Silvia Ferrara

"The Eppendorf BioSpectrometer fluorescence for example was the first device that could enable absorbance as well as fluorescence measurement, which is important since we have many samples in various concentration ranges."

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Eppendorf Client Interview Mi Eun Lee
Eppendorf Client Interview Mi Eun Lee

"I use the Biospectrometer kinetic to measure the OD value to establish the degree of microbial growth after microbial cultivation and to draw the growth curve. Here, the BioSpectrometer has been a very suitable and reliable device to use."

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Purification

Purification

Using the Microcentrifuge 5424 Kit rotor for manual sample purification offers a unique benefit. The rotor is designed with an extra high rim to support open tube lids during centrifugation so that the tube lids don’t tear off. Besides manual purification automated systems are available as well.

  • Automation
    • Same as 5075t, plus
    • Combination of magnetic finger module and Thermomixer
    • Dedicated MagSep reagent kits for nucleic acid purification form 1–24 samples
    • 3 x Prep assistants for user guided automation of nucleic acid purification with MagSep kits
    • 4 x PCR assistants to streamline PCR set-up workflow
  • Centrifuges
    • Max. rotor capacity: 24 x 1.5/2.0 mL vessels
    • Max. speed: 20,238 × g (14,680 rpm)
    • OptiBowl® design for quiet operation, even without a rotor lid
    • Soft-touch one-finger lid closure for ergonomic operation
    • SOFT brake to protect sensitive samples
  • Pipettes and tips
    • Repetitive dispensing modes decrease the number of movements by allowing the user to fill the tip once and dispense up to 100 times
      without a refill
    • Automatic Combitip recognition eliminates timeconsuming volume calculations
    • Wide dispensing range: 1 µL to 10 µL
    • Stress-free work via integrated step counter: dispensing procedures can be continued error-free after an interruption or distraction
    • Central Combitip ejector: fully emptied Combitip can be easiliy ejected with one hand
  • Tubes & Plates
    • Hinged Eppendorf Safe-Lock lid prevents unintentional lid opening during incubation and storage
    • Polypropylene provides high resistance to chemicals, mechanical stress and temperature extremes
    • Manufactured without the use of slip agents, plasticizers, and biocides – substances that have been shown to leach from plastic consumables into the sample and negatively affect bio-assay results
    • g-Safe®: Exceptional centrifugation stability up to 30,000 x g prevents sample loss due to tube breakage and provides extra safety when working with hazardous samples
    • Precise lid sealing for minimal evaporation rates during long-term storage
Quantification

Quantification

A system of Detection instruments and additional equipment allows you to select a combination that fits the required measuring performance. Molecular analysis such as nucleic acid and protein quantification, fluorescence or microvolume measurements can be performed.

  • Photometer
    • UV/Vis spectral range 200 nm to 830 nm
    • Freely selectable wavelengths across the entire UV/Vis spectral range (increment: 1 nm)
    • Compatible with microliter measuring cells and standard cuvettes
    • Temperature controlled cuvette shaft in the BioSpectrometer kinetc
      (20 °C to 42 °C in 0.1 °C increments)
    • Fluorescence intensity measuruements across a range of 0.5 nM to 2,000 nM fluorescein with the BioSpectrometer fluorescence
  • Cuvettes
    • Suitable for measuring small volumes (≥50 µL)
    • UV and Vis-transparent between 220 nm and 1,600 nm
    • Two built-in optical path lengths in a single cuvette-just turn
      the UVette 90° to change from 10 mm and 2 mm
    • Volume markings for 500 µL and 1,000 µL
    • Available in individually packaged PCR clean and protein-free quality
  • Microvolume cuvette
    • Microvolume measuring cell for measuring 1.5 - 10 µL sample volumes
    • Low self-absorption (≤0.05 A @ 260 nm)
    • Hydrophobic surface coating on quartz glass for precise formation and positioning of the sample volume
    • Simple cleaning to minimize the risk of sample carry-over
    • A defined angle for folding the sides of the cuvette quarantees reproducible handling
  • Reader Plates
    • UV-VIS microplates feature an ultrathin film bottom for excellent light transmission in the UV range
    • Black Microplates offer an excellent signal-to-noise ratio - for clear signals even with low-concentration samples
    • White Eppendorf Microplates are optimized for highest sensitivity
      in the detection of luminescence signals by maximizing reflection
    • Solid black and white assay plates are made of polypropylene resulting
      in high resistance to chemicals, mechanical stress and temperature extremes
    • All plates are optimized for minimal autofluorescence and autoluminescence
  • Pipettes and Tips
    • The unique one-button operation offers a fast and ergonomic handling with reduced operating effort and active aerosol reduction. To avoid an accidential tip ejection a clear haptic feedback is provided.
    • The spring loaded tip cone improves user to user reproducibility and improved ergonomics by lowering tip attachment force
    • The Reference 2 multi channel offers a high flexibility incl a spring-loaded tip cone which can be switched on/off optionally
    • The secondary adjustment supports the easy adjustment of your Reference 2 for the most accurate pipetting of different liquids or other external conditions without the need for a full calibration.
    • The quick lock enables an easy cleaning and maintenance of your pipette.
Preparation & Set-up

Preparation & Set-up

The mixing performance of our Thermomixers guarantees efficient mixing of your set-up. Even difficult solutions such as the PCR master mix show perfect results while minimizing the number of bubbles.

  • Thermo mixing devices
    • Heating, mixing and cooling in all common vessel and plate formats
      from 5 µL to 50 mL
    • Increased mixing frequency up to 3,000 rpm (depending on the SmartBlock used)
    • Excellent mixing performance due to unique 2DMix-Control technology
    • Anti-spill technology prevents lid wetting and cross-contamination
    • Reliable prevention of condensate formation with the new ThermoTop (condens.protect®)
  • Centrifuges
    • Rotor capacity: 12 × 1.5/2.0 mL vessels, 2 × PCR strip
    • Max. speed: 14,100 × g (14.500 rpm)
    • Extremely compact footprint
    • Acceleration and deceleration time < 13 s
    • User-friendly digital display for time and speed
  • Tubes and Plates
    • Unmatched, transparent polypropylene (PP) for improved sample visibility
    • OptiTrack® Matrix: 30 % faster sample identification and fewer pipetting errors via high-contrast alphanumeric labeling
    • RecoverMax® well design: Optimized well geometry for minimal remaining volume and excellent mixing properties
    • Raised well rims and smooth surface for reliable closing, even with heat sealing
    • g-Safe®: exceptional centrifugation stability up to 6,000 x g
  • Pipettes and Tips
    • The ultralight Research was developed according to the strict criteria of the Eppendorf PhysioCare Concept
    • Eppendorf PerfectPiston®: Ultralight and highly resistant piston system
    • The positions and shapes of the control buttons/displays have been optimized for stress-free work procedures and intuitive operation
    • The spring-loaded tip cone provides you with an optimal pipette fit with minimal insertion force (not available for 5 mL and 10 mL pipettes)
    • The adjustment option allows you to perfectly adapt the pipette for specific liquids or other external conditions
  • Automation 
    • 0.5 to 300 μL with one system
    • Electronic pipetting with synchronous 96 channel piston movement for better precision and reproducibility or results
    • Auto-detect function for tip size
    • Use 50 μL & 300 μL reload tips for best accuracy
    • Intuitive & industry proven software concept & convenient touch screen control
Amplification & Analysis

Amplification & Analysis

Convenient and extremely fast PCR runs are guaranteed using our Mastercyclers, with or without gradient function for optimization. And with the Detection equipment further analysis such as UV/Vis absorption or fluorescence detection can be conducted.

  • Thermocycler
    • Intuitive graphic programming
    • Small footprint
    • Universal block for ultimate flexibility
    • Connect up to 2 Mastercycler nexus eco to your existing unit
    • Available with gradient
  • PCR Consumables
    • One-piece design: combining a polycarbonate frame and polypropylene wells for optimum performance
    • Extremely thin-walled polypropylene wells guarantee optimum heat transfer to the sample
    • Exceptionally solid and torque-resistant polycarbonate frame
    • Improved well-to-well tolerance
    • Cut-away corner and alphanumeric grid-referencing
  • Pipettes and Tips
    • The unique one-button operation offers a fast and ergonomic handling with reduced operating effort and active aerosol reduction. To avoid an accidential tip ejection a clear haptic feedback is provided.
    • The spring loaded tip cone improves user to user reproducibility and improved ergonomics by lowering tip attachment force
    • The Reference 2 multi channel offers a high flexibility incl a spring-loaded tip cone which can be switched on/off optionally
    • The secondary adjustment supports the easy adjustment of your Reference 2 for the most accurate pipetting of different liquids or other external conditions without the need for a full calibration.
    • The quick lock enables an easy cleaning and maintenance of your pipette.
  • Microvolume cuvette
    • Microvolume measuring cell for measuring 1.5 - 10 µL sample volumes
    • Low self-absorption (≤0.05 A @ 260 nm)
    • Hydrophobic surface coating on quartz glass for precise formation and positioning of the sample volume
    • Simple cleaning to minimize the risk of sample carry-over
    • A defined angle for folding the sides of the cuvette quarantees reproducible handling
  • Photometer
    • UV/Vis spectral range 200 nm to 830 nm
    • Freely selectable wavelengths across the entire UV/Vis spectral range (increment: 1 nm)
    • Compatible with microliter measuring cells and standard cuvettes
    • Temperature controlled cuvette shaft in the BioSpectrometer kinetic
      (20 °C to 42 °C in 0.1 °C increments)
    • Fluorescence intensity measuruements across a range of 0.5 nM to 2,000 nM fluorescein with the BioSpectrometer fluorescence
Storage

Storage

The New Brunswick freezers meet the highest standards for quality in regard to performance, sample security, and convenience. To concentrate your samples prior to the storage, the Concentrator plus offers an advanced technology and assures quick, efficient and gentle vacuum concentration.

  • Freezer
    • Provides 30% more storage capacity than freezers of equal size
    • Inner doors in upright models are gasketed and insulated creating three separate compartments minimizing cold air loss
    • Unique heated air vent with plunger to clear ice buildup and to relieve vacuum formation to gain immediate access to the freezer
    • Built-in voltage stabilizer on U535, U725, C585, and C760 freezers provides extra sample security
  • Deepwell Plates
    • OptiTrack® matrix: 30 % faster sample identification and fewer pipetting errors via high-contrast alphanumeric labeling
    • Conical RecoverMax® well design: Optimized well geometry for maximum sample recovery and excellent mixing properties
    • Minimal remaining volume and high uniformity from well to well
    • Raised well rims and smooth surface for reliable closing, even with heat sealing
    • Easy and reliable stacking
  • Conical tubes
    • Premium USP class 6 raw material and no use of slip agents, plasticizers, and biocides during manufacturing ensure highest sample integrity, high...
    • Cap with flattened and grooved sides supports a user-friendly ergonomic handling, enables a stable upright positioning, and minimizes contamination risk.
    • Reliable and safe labelling due to a large writing area on the tube and due to a flat and light-colored cap.
    • Precise dimensions enable maximum compatibility with centrifuge rotors, mixers and shakers.
    • Sterile, pyrogen-, DNase-, RNase-, and DNA-free. Each individual lot is checked and certified for quality and purity
  • Sealing options
    • Storage Film and Foil glued seals for simple and dependable sealing during sample storage
    • Heat Sealing Film and foil for continuous locking with the best evaporation protection (see heat sealing materials)
    • Foil products are made of aluminum, can be pierced, and protect light-sensitive samples
    • Film products are transparent and provide protection against unwanted punctures
    • Eppendorf Plate Lid: stable, flexible protection of samples during short-term storage
  • Concentrator
    • Comes in 3 system configurations
    • Extremely quite operation <50 dB(A)
    • Chemical-resistant, maintenance-free PTFE diaphragm pump eliminates the need for changing up pump oil
    • Small footprint saves valuable bench space
    • Wide variety of 15 different rotor options to accommodate tubes
      from 0.2 mL to 50 mL, microplates and PCR plates