Three-dimensional (3D) cell aggregates are of great interest for many applications, including disease modeling, drug toxicity assessment, and manufacture of stem cell-based products. Stirred-tank bioreactors are promising culture systems for 3D cell aggregates, as they allow efficient establishment and maintenance of cell aggregates, process monitoring and control, and process scale-up to larger volumes. Furthermore, they can be operated in perfusion mode, which allows 3D cell aggregates to be sustained longer than in traditional batch cultures.
This webcast will review a research example for process development with the human tumor cell line H157, cultivated in stirred-tank mini bioreactors as 3D cell aggregates.
Key Learning Objectives
- Learn about the challenges and benefits of perfusion cultivation for stem cells and 3D cell models
- Gain insight on the impact of impeller geometry on cell growth and aggregate formation
- Interact with an expert in a live Q&A session