Are Eppendorf products free of RNA?

All products certified with "PCR clean" are tested free of human DNA, DNase, RNase and PCR inhibitors. The following applies from our point of view: Where no human DNA is detected, there can also be no RNA, which is considerably more unstable.

What is the meaning of the PCR Clean seal?

For molecular-biological requirements Eppendorf offers a new standard for uniform purity specifications. All consumables marked with the "Certified PCR Clean” label are guaranteed free of human DNA, DNase, RNase and PCR inhibitors.

Why are there double labels on the folding boxes, as "PCR clean" + "Sterile" or "PCR clean" + "Protein-free"?

Due to the modularization of purity grades, some products are labeled with more than one purity grade symbol. For example, the epDualfilter T.I.P.S. has the double seal "PCR clean "+ "Sterile" and the UVette the double seal "PCR clean" + "Protein-free" on the packing. The certification remains unaffected by the modularization.

Are lot specific certificates for the purity grades available in the internet?

Yes, the lot specific purity certificates, so-called Test report, can be downloaded at the certificates homepage .

Can products of the previous and new modular purity grades (with new seals) be used simultaneously in one application?

Yes, if no batch attachment is required for the application, products of the old and new modular purity grades can be used, as their specifications are the same.

Which advantages do Eppendorf Polypropylene Microplates have over plates made from polystyrene?

Plates made from polypropylene may be used across a broad spectrum of applications, as they are more resistant to temperature variations as well as various chemicals than plates made from polystyrene. Furthermore, they are more robust, allowing them to be centrifuged at higher g-forces. In addition, biomolecules, such as nucleic acids and proteins, bind less strongly to polypropylene.

Are Eppendorf PP Microplates resistant to organic solvents, e.g. DMSO?

The Eppendorf PP Microplates are made from polypropylene and are thus resistant to DMSO and other organic solvents, unlike plates made from polystyrene. An overview of chemical resistance can be found in Application Note no. 56.

Are the colored rim and labeling resistant to organic solvents?

All areas of the plate, including the colored rim, are resistant to chemicals. The alpha numeric labeling is equally durable. An overview of chemical resistance can be found in Application Note no. 56.

How may the Eppendorf PP Microplates be sealed?

Eppendorf offers a variety of sealing options for Microplates. Self-adhesive sealers, films and foils for heat sealing, as well as re-usable sealing mats, are available. Information regarding the compatibility of the different sealing methods is listed in the Application Note no. 208.

Which advantages do the black Eppendorf PP Microplates offer?

Plates made from black material are especially well suited for fluorescence assays (e.g. PicoGreen ® ). The black wells prevent “cross talk” between wells and reduce background signals. The black Eppendorf PP Microplates feature the unique OptiTrack Matrix, the first technology to allow for fast and easy well identification in black plates. Due to carefully selected materials, the plates have very low autofluorescence. The superior signal-to-noise ratio contributes to the increased sensitivity of fluorescence assays (see Application Note no. 203).

Which advantages do the white Eppendorf PP Microplates offer?

Plates made from white material may be used for luminescence assays (chemiluminescence, scintillation proximity assays (SPA) etc.), as well as for fluorescence assays (fluorescence intensity, polarization, etc.). The white wells prevent “cross talk” between the wells while amplifying the individual signals. The white Eppendorf PP Microplates feature the unique OptiTrack Matrix, the first technology to allow for fast and easy well identification in white plates. Due to carefully selected materials, the plates offer maximum signal amplification. Due to their very low autofluorescence, the white Eppendorf Microplates are equally well suited for fluorescence assays. Taken together, these features allow for an improvement in the sensitivity of analyses.

May the Eppendorf PP Microplates be stored at –196°C in liquid nitrogen?

No, we recommend storage at a minimum of –80°C.

May the Eppendorf PP Microplates be autoclaved?

Yes; however, the exact specifications of the Microplates could be compromised. The plates are available in the qualities sterile or PCR clean. Hence, autoclaving is necessary only under exceptional circumstances.

Are the Eppendorf PP Microplates in LoBind quality coated or siliconized?

No, the LoBind quality of the Eppendorf PP Microplates is not derived from siliconization or any other type of coating. The functionality is achieved through a carefully selected material, in combination with a special manufacturing process.

How high may Eppendorf PP Microplates be centrifuged?

The Eppendorf PP Microplates may be centrifuged up to 6000 x g. Please follow the instructions provided in the manual.

May immunoassays such as ELISA be performed in the Eppendorf PP Microplates?

Plates made from polypropylene are not suited for the standard variant, where antigen or antibodies are bound to the well wall. For this application, plates made from polystyrene are used, which have a higher binding capacity for biomolecules. However, for antibody-based fluorescence or luminescence assays (e.g. with beads) Eppendorf PP Microplates with black or white wells are suitable, respectively.

Is it possible to perform absorption measurements in the Eppendorf PP Microplates?

Typically, absorption measurements are not performed in plates made from polypropylene, but in plates made from polystyrene, as this material generally has a very high transparency. Since the Eppendorf PP Microplates show considerably higher transparency compared to other plates made from polypropylene (see Application Note no. 202), the Eppendorf Microplates may be used for absorption measurements in certain cases.

Why are the Eppendorf PP Microplates offered in Sterile and PCR clean, but not in Eppendorf quality?

The Eppendorf PP Microplates are a very high quality product. In order to underline the quality, all plates are available in the degrees of purity PCR clean or Sterile. This increases the confidence and comfort of the user, who may employ the plates directly for valuable samples in sophisticated applications.

Are Eppendorf PP Microplates also available with barcode?

Yes, the option of Eppendorf Microplates with barcode is available. Further information can be found on the product page microplates .

Are Eppendorf tubes and plates (also LoBind variants) resistant to 100% acetonitrile?

Eppendorf tubes and plates including LoBind variants are resistant against 100% acetonitrile only in room temperature conditions. Above 40°C and prolonged exposure (days) may lead to partial damage and is not recommended. For lower acetonitrile concentrations the resistance parameters will be better so prolonged exposure and higher temperatures may be possible. This must be however tested by the customer.

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Eppendorf Microplates

OptiTrack® matrix를 지원하는 Eppendorf Microplate

4개의 microplate, 384-well 검은색 및 투명 plate, 96-well 흰색 및 투명 plate

Combitip® 피펫 팁을 사용해 microplate로 시료를 피펫팅하는 모습

자동화된 liquid handling 기기에서 처리 중인 Eppendorf Microplate

제품 정보

Eppendorf Microplates의 탁월한 투명도를 경험해 보세요! 고품질 폴리프로필렌과 탁월한 well 디자인으로 시료의 피펫팅, 제어 및 회수가 쉽고 간편해집니다. 또한 Eppendorf의 plate는 모든 실험에 적합합니다.

제품 정보 자세히 표시

이 페이지 하단에서 추가 다운로드 자료를찾을 수 있습니다.

제품 정보

애플리케이션

특징

Eppendorf Microplates를 사용하면 시료의 피펫팅, 제어 및 회수가 간편해집니다! 가독성이 뛰어난 OptiTrack^® matrix 라벨 덕분에 각 well의 식별이 쉬워지고 주입 시간이 단축되며 피펫팅 실수 위험이 현저히 감소합니다. Eppendorf Microplates는 투명도가 우수한 폴리프로필렌을 사용해 제조되어 시료 위치를 쉽고 빠르게 확인할 수 있고, Eppendorf LoBind^®로도 제공되어 시료 회수를 극대화할 수 있습니다.

탁월한 투명도와 OptiTrack^® matrix

Eppendorf Microplates는 최고 품질의 폴리프로필렌으로 제조되어 최대의 투명도를 보장하고 재현성을 높입니다. 가독성이 뛰어난 OptiTrack matrix로 well을 신속하게 식별하고 실수 및 오염 위험을 줄일 수 있어 실험실의 효율성이 향상됩니다.
Eppendorf Microplates를 사용해 시료를 얼마나 빨리 식별할 수 있는지 알아보려면 Application Note 202( Eppendorf Polypropylene Microplates – 빠르고 안정적인 시료 식별)를 참조하십시오.

가장 짧은 시간에 회수율을 극대화하는 RecoverMax^® well 디자인

Eppendorf microplate의 RecoverMax^® well은 최대의 시료 회수율과 탁월한 혼합 특성을 보장합니다. Well 코너 디자인이 둥글고 매끄러워 시료 손실을 방지하고 잔여량을 최소화할 수 있을 뿐만 아니라 사각형 well plate의 매끄러운 윤곽으로 교차 오염 위험이 거의 없습니다.

Eppendorf Microplates는 다양한 타입으로 제공되며 탁월한 투명도와 품질을 보장합니다.

• 96 및 384 well 타입
• F–bottom (flat), U–bottom (round), V–bottom (conical).
• Eppendorf PCR clean 및 Sterile purity grade
• DNA 및 Protein LoBind로도 제공
• g–Safe^®: 최대 6,000 x g의 탁월한 원심분리 성능으로 프로토콜 속도 및 시료 품질 향상.
• 위로 올라간 형태의 웰 테두리와 균일한 표면이 Heat Sealing으로도 확실한 밀봉 보장.
• 화학물질, 기계적 응력 및 극한 온도에 대한 저항성이 뛰어난 고품질 폴리프로필렌.
• 오토클레이브 가능(121 °C, 20 분).
• 이형제, 연화제 또는 항균제를 사용하지 않고 제조.

추가 정보는 Application Note 56 참조: 오리지널 Eppendorf Tubes^® 및 Plates에 사용된 최고의 소재: 폴리프로필렌의 특성 및 내화학성. Application Note 239: Eppendorf Deepwell Plates 및 Microplates - sealing 방법 조사.