How many parameters can I control using a DASGIP or DASbox system?

DASGIP Parallel Bioreactor Systems provide comprehensive concurrent monitoring and control of process parameters. During the process run, events are logged together with up to 70 online process variables per bioreactor. In addition to the various DASGIP modules for monitoring and control, with DASware analyze users can integrate analytical devices provided by third parties. Seamless integration into the DASGIP or DASbox system even allows for closed loop solutions such as automated feeding strategies based on the current glucose concentration. Each process value can be used as a trigger enabling users to automate their process.

How many parameters can be displayed in a process overview in DASware control?

DASware control users can freely select all relevant process variables of all or selected bioreactors to be displayed in one chart. There are no limitations in the number of process parameters. When using DASware analyze, the displayed process parameters even include integrated data from third party laboratory devices, such as nutrient analyzers, mass spectrometers or Raman. The software features tables and trend graphs of all measured values. It continuously logs and stores all data and events. These data are immediately accessible for evaluation using e.g. Microsoft Excel. Advanced charting functionalities ease further data analysis and comparison of runs.

Do I need programming skills to automate my process in DASware control?

DASware control includes a set of easy to implement automation features without any need of programming. Profiles and triggers allow for various control strategies, all of them easy to select by mouse click with parameters entered manually or just copy-pasted. Users with programming skills and special control requirements, however, are free to run scripted control strategies and profiles as well.

Can I change triggers, profiles, cascades and scripts online in DASware control?

Set-points, profiles, cascades, and scripts are online editable in DASware control. Offline values and events can be entered during or after the run and documented with a time stamp. These time stamps can be set individually, e.g to meet the time, when the sample was taken. This provides users with great flexibility to interact with the control system and reconsider parameter settings even in highly automated processes. All events, including online changes, are recorded in a SQL Server database.

How are DASGIP vessels agitated?

DASGIP bioreactors feature powerful overhead drives with agitation speeds ranging 30 – 1,600 rpm. The DASGIP TC4SC4 controls temperature and agitation in up to four bioreactors. Users can individually define each outlet set-point as well as choose from different operation modes (e.g. continuous or interval agitation).

Does DASware control give any alarms in case of critical deviation?

Critical deviation of process parameters such as pH, DO, redox, and temperature are color-coded in the software and saved in the event log. DASware control professional additionally allows notification of supervisory control systems or mobile communication tools via SMS or e-mail.

Can you help adjusting my controller loop parameters in DASware control?

DASGIP controllers are PI controllers, which are configured with default controller parameters adapted to the type of vessel and default experimental requirements. Depending on special customer requirements it might be necessary to modify these parameters. Our service will be happy to help you finding the right settings.

How to calibrate a pH sensor in DASware control?

DASware control software allows for parallel calibration of all sensors used within one system with all data logged in the central database together with set-points, profiles, cascades, process values, user interactions and other events. Good maintenance will prolong the sensors' lifetime whereas high temperatures (i.e. during autoclavation), alkaline solutions (i.e. during alkaline deactivation), repeated etchings and improper maintenance will reduce the electrode's lifetime.

How reliable are exhaust data when working at benchtop scale?

Exhaust oxygen and carbon dioxide concentrations of multiple bioreactor units are continuously monitored with the DASGIP GA exhaust analyzer. Together with the mass flow measurement and the input values of oxygen and carbon dioxide concentrations, automatic calculation of oxygen transfer rate (OTR), carbon dioxide transfer rate (CTR), and respiratory quotient (RQ) is possible, permitting direct conclusions on the metabolic state of a culture. As the DASGIP GA considers humidity, volume, and total flow typical sources for inaccurate OTR and CTR data in small working volumes are eliminated.

Is it possible to automatically control the foam in DASGIP vessels?

All DASGIP vessels can be equipped with foam control components. All you need is a level/foam sensor connected to the DASGIP sensor module (e.g. PH4PO4L) and a feed line for dosing of the antifoam agent. Now foam formation can be detected by the level sensor and antifoam agent is automatically pumped into the vessel until all foam is collapsed. The DASware control software allows any feed line to be used for antifoam or level control. Since medium composition has a strong impact on the electrical conductivity of medium and foam, every conductivity-based level sensor needs to be adjusted to foam and medium conductivity. DASware control supports monitoring conductivity and free adjustment of the conductivity threshold.

What maintenance is required for Eppendorf bioprocess systems?

As all complex technical systems Eppendorf bioprocess equipment should be maintained regularly to keep all parts in good condition. This maintenance avoids cost-intensive down times and contributes to preservation of value. A complete preventive maintenance is recommended once a year. Additional maintenance actions are recommended to be executed by the user prior to every run or in regular cycles (e.g. every month). Our service will be happy to advice.

What do I have to consider when returning equipment for repair?

Equipment returned to Eppendorf for service or any other reason needs to be decontaminated first. Please fill out the Decontamination Form that you will find in the “Service & Support” section of the Eppendorf website and attach it to the package. Equipment which was sent without the completely filled form cannot be handled. This form must also be completed before any on-site service, whether covered under warranty or not, can commence.

What does your warranty cover?

Eppendorf issues a warranty of one year from the day of delivery on its products in accordance with our terms and conditions. Excluded are consumables such as cables, tubing, glassware, and sensors as well as used material.

Does Eppendorf offer bioreactor training?

We highly recommend a training at the customer’s site for all new customers. Besides this initial training, Eppendorf also offers individual trainings adapted to the user’s requirements like training of new employees or advanced training/recapitulation for employees already having experience with DASGIP or DASbox systems. All trainings are performed at the customer’s site using the customer’s equipment to have the training focused on the customer’s demands. The structure and methods of the training can be chosen to the customer’s requirements from general overview to detailed sessions on specific products or issues. Training in small groups is as possible as personal guidance.

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DASbox^® Mini Bioreactor System

BioBLU c and BioBLU f Single-Use Bioreactors for small and bench scale cell culture, stem cell, and fermentation applications.

제품 정보

Eppendorf DASbox Mini Bioreactor System은 소용량, 소형 디자인 그리고 고급 제어 및 정보 관리 옵션으로 연구 및 초기 프로세스 개발 단계의 요구사항을 충족합니다. DASware Software Suite와 함께 DoE(Design of Experiments) 접근법과 프로세스 개발의 표준화 및 간소화를 위한 최적의 시스템을 구성합니다.

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특징

DASbox는 미생물 및 세포 배양 실험과 줄기세포 실험에 적합한 소형 바이오리액터 시스템입니다. 최대 24개의 병렬식 바이오리액터를 사용할 수 있는 4단 시스템으로 설계되었습니다. 용량이 60 – 250 mL인 DASbox는 고급 프로세스 개발과 DoE(Design of Experiments) 작업에 이상적인 도구입니다. 모든 중요 프로세스 파라미터를 정밀하게 제어할 수 있습니다. 무액체 온도 제어와 배기 응축으로 취급이 간편합니다.
산업 표준 유리 바이오리액터를 사용하는 것 외에도 DASbox에 Eppendorf BioBLU 0.3 Single-Use Bioreactors를 장착할 수 있습니다. 모든 장비가 완비된 일회용 미니 바이오리액터입니다.

사양	DASbox^® Cell Culture	DASbox^® Cell Culture 일회용	DASbox^® 미생물	DASbox^® 미생물 일회용

용도	Cell Culture	Cell Culture	미생물	미생물
병렬 유닛 수	최대 24개	최대 24개	최대 24개	최대 24개
소프트웨어	DASware control, 기타 DASware 옵션	DASware control, 기타 DASware 옵션	DASware control, 기타 DASware 옵션	DASware control, 기타 DASware 옵션
사용자 인터페이스	모니터가 장착된 프로세스 컴퓨터	모니터가 장착된 프로세스 컴퓨터	모니터가 장착된 프로세스 컴퓨터	모니터가 장착된 프로세스 컴퓨터
일반 소비 전력(4단 시스템, 프로세스 컴퓨터 미포함)	168 W (@ 230 V)/ 154 W (@ 115 V)	168 W (@ 230 V)/ 154 W (@ 115 V)	168 W (@ 230 V)/ 154 W (@ 115 V)	168 W (@ 230 V)/ 154 W (@ 115 V)
치수(W × D × H, 4단 시스템, 프로세스 컴퓨터 미포함)	30 x 70 x 49 cm (12 x 28 x 19 in)	30 x 70 x 49 cm (12 x 28 x 19 in)	30 x 70 x 49 cm (12 x 28 x 19 in)	30 x 70 x 49 cm (12 x 28 x 19 in)
제품 무게	44 kg	44 kg	44 kg	44 kg
일반 무게	44 kg	44 kg	44 kg	44 kg
박테리아/효모/균류	예	예	예	예
포유류/동물	예	예	예	예
곤충	예	예	예	예
교체형 Vessel	예	예	예	예
검증성	아니요	아니요	아니요	아니요
줄기 세포	예	예	예	예

Bioreactor
Vessel	유리 Vessel	일회용 Vessel	유리 Vessel	일회용 Vessel
멸균	오토클레이브 가능	사전 멸균	오토클레이브 가능	사전 멸균
총 용량	350 mL을 사용한 정밀한 one-step 피펫팅	380 mL을 사용한 정밀한 one-step 피펫팅	350 mL을 사용한 정밀한 one-step 피펫팅	380 mL을 사용한 정밀한 one-step 피펫팅

교반
드라이브	다이렉트 오버헤드 드라이브	마그네틱 오버헤드 드라이브	다이렉트 오버헤드 드라이브	마그네틱 오버헤드 드라이브
속도 범위	20 – 2500 rpm	20 – 500 rpm	20 – 2500 rpm	20 – 2000 rpm
임펠러	선박	피치 블레이드	Rushton 타입	Rushton 타입

기체 주입
기체 공급	TMFC; 오버레이 및/또는 스파저	TMFC; 오버레이 및/또는 스파저	TMFC; 스파저	TMFC; 스파저
표준 기체 유량	0.04 – 5 sL/h, 0.04 – 3.5 sL/h CO₂	0.04 – 5 sL/h, 0.04 – 3.5 sL/h CO₂	0.2 – 25 sL/h, 0.2 – 18 sL/h CO₂	0.2 – 25 sL/h, 0.2 – 18 sL/h CO₂
표준 기체 Mixing	Air, N₂, O₂ 및/또는 CO₂	Air, N₂, O₂ 및/또는 CO₂	Air, N₂, O₂ 및/또는 CO₂	Air, N₂, O₂ 및/또는 CO₂

공급
Vessel당 공급 라인	2 (기본사항)/ 4 (선택사항)	2 (기본사항)/ 4 (선택사항)	2 (기본사항)/ 4 (선택사항)	2 (기본사항)/ 4 (선택사항)
표준 공급 속도(튜브 직경에 따라 다름)	0.3 – 9.5 mL/h - 13 – 420 mL/h	0.3 – 9.5 mL/h - 13 – 420 mL/h	0.3 – 9.5 mL/h - 13 – 420 mL/h	0.3 – 9.5 mL/h - 13 – 420 mL/h

모니터링 및 제어
온도 제어	무액체 가열 및 냉각(Peltier)	무액체 가열 및 냉각(Peltier)	무액체 가열 및 냉각(Peltier)	무액체 가열 및 냉각(Peltier)
표준 온도 범위(25° C 실온)	10 – 60°C	10 – 45°C	10 – 60°C	10 – 45°C
pH 제어	CO₂/염기 및 기타 셋업	CO₂/염기 및 기타 셋업	산 및/또는 염기, 및 기타 셋업	산 및/또는 염기, 및 기타 셋업
DO 제어	캐스케이드(O₂ 농도, 기체 유량) 및 기타 셋업	캐스케이드(O₂ 농도, 기체 유량) 및 기타 셋업	캐스케이드(교반 속도, O₂ 농도, 기체 유량) 및 기타 셋업	캐스케이드(교반 속도, O₂ 농도, 기체 유량) 및 기타 셋업
ORP (redox) 측정	-	-	선택사항(산화환원과 레벨 중 선택)	선택사항(산화환원과 레벨 중 선택)
레벨/거품	선택사항	선택사항	선택사항(산화환원과 레벨 중 선택)	선택사항(산화환원과 레벨 중 선택)
OD 측정	선택사항 (DASGIP OD4)	선택사항 (DASGIP OD4)	선택사항 (DASGIP OD4)	선택사항 (DASGIP OD4)
배기 응축	무액체(Peltier)	무액체(Peltier)	무액체(Peltier)	무액체(Peltier)
배기 분석	-	-	선택사항 (DASGIP GA4)	선택사항 (DASGIP GA4)