Are Eppendorf consumables free of RNA?

All consumables certified with "PCR clean" are tested free of human DNA, DNase, RNase and PCR inhibitors. The following applies from our point of view: where no human DNA is detected, there can also be no RNA, which is considerably more unstable.

Are Eppendorf tubes, plates (also LoBind variants), and pipette tips resistant to 100% acetonitrile?

Eppendorf tubes, plates including LoBind, and pipette tips are resistant to 100% acetonitrile only in room temperature conditions. Above 40°C and prolonged exposure (days) may lead to partial damage and is not recommended. For lower acetonitrile concentrations the resistance parameters will be better so prolonged exposure and higher temperatures may be possible. This must be however tested by the customer.

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PCR films & foils

Masterclear real-time PCR film으로 밀봉된 96-well plate

제품 정보

모든 PCR plate를 완벽하게 밀봉해 보십시오. Eppendorf PCR film 및 foil은 PCR plate의 완벽한 밀봉을 보장합니다. Assay에 가장 적합한 sealing 소재를 선택하십시오. 예를 들어, 광학적 특성이 뛰어난 Masterclear^® real-time PCR film은 qPCR을 위한 완벽한 선택입니다.

제품 정보 자세히 표시

이 페이지 하단에서 추가 다운로드 자료를찾을 수 있습니다.

제품 정보

애플리케이션

PCR Film 및 Foil은 Plate를 효과적이고 완벽하게 밀봉합니다.
Eppendorf PCR film 및 foil은 효과적으로 plate를 밀봉해 서멀 사이클링과 보관 중에 PCR 시료를 보호합니다. PCR plate seal은 뛰어난 내화학성을 제공하고 다양한 실험 물질에 대한 저항성 테스트를 거쳤습니다. 최적의 광학적 특성을 갖는 특수한 seal을 사용해 qPCR에서 신호 손실을 최소화합니다.

자세한 정보는 사용자 가이드 043(PCR Plates, Deepwell Plates 및 Microplates용 Sealing 시스템의 내화학성 조사)을 참조하십시오.

선택하는 데 어려움이 있나요? Eppendorf는 microwell plate를 위한 다양한 sealing 시스템을 제공합니다.

• 접착식 film 또는 foil
• sealing mat
• plate lid
• heat-sealing film 또는 foil

Adhesive films or foils

• Adhesive films or foils are glued the plate surface.
• Simple handling; however, diligent and equal pressure are necessary to ensure good protection from evaporation.
• Possible influence of the adhesive on the sample has to be taken into consideration.
• Optimal adhesion only at PCR temperatures and should not be used for storage applications.

Sealing mats

• Reusable mats made from an elastomer.
• The plate can be opened and re-sealed at any time.
• The mat needs to be compatible with the plate (round/square wells).

Plate lids

• Reusable transparent lid made from polystyrene.
• Simplest and fastest closure option.
• Mainly used as a temporary cover and for short incubations, since protection from evaporation is limited compared to other sealing options.

PCR Film and Foil (self-adhesive)Heat-sealing foils and films (requires a plate sealer)

• Heat-sealed foils and films are welded to the plates using a heat-sealing device.
• Very secure and effective heat seal.
• Only suitable for plates made from polypropylene or similar materials (including Eppendorf twin.tec^® PCR Plates)
• A raised rim around the individual wells is required for heat seals.

Which sealing system should I choose for my PCR plates?

PCR sealing film or foil?

The right sealing option will highly depend on your intended application. The first choice you should make is whether foils or films are most suitable. In general, foils are well-suited for light-sensitive samples. Foils can also be easily pierced, meaning your samples can be transferred via a needle or automated system. On the other hand, films are a more practical option if you need transparency for optical measurements (e.g. qPCR) and protection from unintended piercing.

Permanent or temporary sealing?

Another key consideration is whether your seal needs to be permanent or temporary. If you need permanent sealing, heat-sealing foils and films are a durable long-term option, providing strong protection against evaporation. For applications requiring temporary sealing, self-adhesive options and sealing mats are an ideal choice, offering easy, residue-free removal.

Conducting qPCR?

Masterclear real-time PCR film is optimized for maximum light transmission. Transmission values of > 90 % between 350 nm and 750 nm guarantee optimal excitation of your fluorescent dyes and optimal readout of the emitted fluorescence.

Using automated systems?

Our Heat Sealing Foils are made of aluminum. They provide a strong heat seal that prevents evaporation, even with low sample volumes. They also can be easily pierced by autosamplers, making them particularly well-suited for high-throughput automated assays.

Sealing option selection guide

Still stuck on which sealing option to choose? Explore the table below for more information to help you choose the best sealing option for your application.

^{Table 1: Eppendorf sealing option selection guide, outlining the main features of each product. The guide includes self-adhesive films and foils, heat-seal films and foils, storage films and foils, sealing mats, plate lids, and options for real-time PCR.}