Pipetting knowledge hub

Library Preparation for Next Generation Sequencing (NGS) involve readying available DNA or RNA samples for sequencing. Depending on the research goal, e.g. whole genome or whole exome sequencing, workflows vary in detail but follow the same general principal. This includes normalization, fragmentation, and adapter ligation. Samples then undergo size-selection and are finally amplified. Lastly, quality control is being done before loading the sequencer's flow cell.
However, what all NGS library prep workflows have in common is the need for hundreds of precise pipetting tasks, be it for reagent addition, bead handling, or normalization steps. All the time while using costly reagents and having strict requirements towards reproducibility.

Challenges in NGS

• Tedious, costly workflows that require high focus over hours
• Risk of cross-contamination and sample mix-up
• Demand for consistent results and traceability
• Handling precious samples, low volumes, and challenging liquids