Adapting Centrifuge Time and Speed

Academia de laboratorio 14. febrero 2018

What can you do if your centrifuge and rotor provide, e.g., only lower g × force than your protocol requires? You can centrifuge for an appropriate longer length of time. But what centrifuge time and speed will be required? And how do you calculate this? The answer: by using the k-factor, a measure of the sedimentation distance. This will tell you how long it will take the particles to settle at the bottom of the test tube.

The k-factor represents the pelleting efficiency of a centrifugation system at maximum rotational speed. You can download our Centrifuge Family Overview to find out the maximum speed of your Eppendorf centrifuge.
The k-factor is a measure of the sedimentation distance in a test tube; it depends on the difference between the longest and the shortest distance between the sample and rotor axis. The shorter the sedimentation distance, the shorter the centrifugation time. In addition, the angle of the tubes within the rotor influences the sedimentation distance.

The radius of the rotor and the angle of the bores as well as the type of vessel respectively the adapter you use will all have an impact on the sedimentation distance and thus on the centrifugation time you will need.
The k-factor is a measure of the sedimentation distance and tells us how long it will take until the particles settle at the bottom of the test tube.
The smaller the k-factor, the better the pelleting efficiency.

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The formula for calculating the k-factor considers these parameters:

formel_1_calculating_the_relative_centrifugation_time

Maximum rotor radius

Minimum rotor radius

Speed

To achieve the same sedimentation result and compensate for not having g × force, you can calculate how much longer to centrifuge using a formula based on the k-factors of both rotors and the centrifugation time of the rotor originally used.

Is adjusting your centrifuge speed and time getting in the way of your experiments? You can also use our calculation tool to measure the required rotational speed and relative centrifugal force, using the rotor diameter.

Learn more basics in centrifugation

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Adapting Centrifuge Time and Speed