Optimize Your Bioprocess – From shake flask to bioreactor

Bioreactors and shakers are used to cultivate microorganisms, plant, insect, and mammalian cells in different volumes. Upscaling of production often involves shake flasks and bioreactors.

Shake flasks are used for bioreactor inoculum production and are an easy-to-use instrument for early process development like organism screening and media design. At the next process steps, highly controllable bioreactors are needed for flexible solutions, automated process control, and predictable scalability when larger quantities of cells are required. The challenge during upscaling is to keep a constant efficiency and quality during all process steps.

In this webinar, we will depict the typical applications of shakers and bioreactors in an upscaling bioprocess. We will highlight applicational differences and factors that influence cultivation and system transition. Furthermore, we will introduce to you the basic components of a bioreactor and the various process parameters that can be controlled. Finally, we will go through different process strategies to further optimize a process and increase production.

Target groups:

Beginners, who start using shake flasks and bioreactors for cultivation and upscaling
Early bioreactor users, who want to deepen their knowledge and understanding of the possibilities of process control strategies

Covered areas in the session:

Learn about typical applications of shake flasks and stirred-tank bioreactors in bioprocess development
Learn about influencing factors and optimizing setscrews in shaking/stirring
Learn about the basic bioreactor components and the different process parameters that can be monitored and controlled, and what they tell you about your culture
Learn about feeding possibilities and their impact on your culture
Live “Ask the Expert” session – Deepen your knowledge and optimize your process

Speakers

Ines Hartmann, Application Specialist Cell Handling, Eppendorf

Ines Hartmann is an application specialist for cell handling and responsible for the shakers and incubators at the Eppendorf headquarter in Hamburg, Germany. She joined the company in 2008 and has worked on several topics in cell handling, liquid handling and consumables. She obtained her diploma degree in biology at the Humboldt University of Berlin, Germany and gained expertise in various laboratory techniques.

Igor Vassilev, PhD, Application Lab Manager, Eppendorf Bioprocess

Igor Vassilev studied Chemical Biology at the University of Dortmund (Germany). He did his PhD in Biotechnology at the University of Queensland (Australia) on the topic of microbial electrosynthesis. After several stations as a laboratory coordinator in Queensland (Australia), visiting scientist in Wageningen (Netherlands) and a postdoc position in Tampere (Finland), he started his career at Eppendorf as Application Laboratory Manager beginning of 2023.