Are Eppendorf consumables free of RNA?

All consumables certified with "PCR clean" are tested free of human DNA, DNase, RNase and PCR inhibitors. The following applies from our point of view: where no human DNA is detected, there can also be no RNA, which is considerably more unstable.

Are lot specific certificates for the purity grades available in the internet?

Yes, the lot specific purity certificates, so-called Test report, can be downloaded at the certificates homepage .

Which advantages do Eppendorf Polypropylene Microplates have over plates made from polystyrene?

Plates made from polypropylene may be used across a broad spectrum of applications, as they are more resistant to temperature variations as well as various chemicals than plates made from polystyrene. Furthermore, they are more robust, allowing them to be centrifuged at higher g-forces. In addition, biomolecules, such as nucleic acids and proteins, bind less strongly to polypropylene.

How may the Eppendorf PP Microplates be sealed?

Eppendorf offers a variety of sealing options for Microplates. Self-adhesive sealers, films and foils for heat sealing, as well as re-usable sealing mats, are available. Information regarding the compatibility of the different sealing methods is listed in the Application Note no. 239 .

Which advantages do the black Eppendorf PP Microplates offer?

Plates made from black material are especially well suited for fluorescence assays (e.g. PicoGreen ® ). The black wells prevent “cross talk” between wells and reduce background signals. The black Eppendorf PP Microplates feature the unique OptiTrack Matrix, the first technology to allow for fast and easy well identification in black plates. Due to carefully selected materials, the plates have very low autofluorescence. The superior signal-to-noise ratio contributes to the increased sensitivity of fluorescence assays (see Application Note no. 203 ).

Which advantages do the white Eppendorf PP Microplates offer?

Plates made from white material may be used for luminescence assays (chemiluminescence, scintillation proximity assays (SPA) etc.), as well as for fluorescence assays (fluorescence intensity, polarization, etc.). The white wells prevent “cross talk” between the wells while amplifying the individual signals. The white Eppendorf PP Microplates feature the unique OptiTrack Matrix, the first technology to allow for fast and easy well identification in white plates. Due to carefully selected materials, the plates offer maximum signal amplification. Due to their very low autofluorescence, the white Eppendorf Microplates are equally well suited for fluorescence assays. Taken together, these features allow for an improvement in the sensitivity of analyses.

May the Eppendorf PP Microplates be stored at –196°C in liquid nitrogen?

No, we recommend storage at a minimum of –80°C.

May the Eppendorf PP Microplates be autoclaved?

Yes; however, the exact specifications of the Microplates could be compromised. The plates are available in the qualities sterile or PCR clean. Hence, autoclaving is necessary only under exceptional circumstances.

Are the Eppendorf PP Microplates in LoBind quality coated or siliconized?

No, the LoBind quality of the Eppendorf PP Microplates is not derived from siliconization or any other type of coating. The functionality is achieved through a carefully selected material, in combination with a special manufacturing process.

How high may Eppendorf PP Microplates be centrifuged?

The Eppendorf PP Microplates may be centrifuged up to 6000 x g. Please follow the instructions provided in the manual.

May immunoassays such as ELISA be performed in the Eppendorf PP Microplates?

Plates made from polypropylene are not suited for the standard variant, where antigen or antibodies are bound to the well wall. For this application, plates made from polystyrene are used, which have a higher binding capacity for biomolecules. However, for antibody-based fluorescence or luminescence assays (e.g. with beads) Eppendorf PP Microplates with black or white wells are suitable, respectively.

Is it possible to perform absorption measurements in the Eppendorf PP Microplates?

Typically, absorption measurements are not performed in plates made from polypropylene, but in plates made from polystyrene, as this material generally has a very high transparency. Since the Eppendorf PP Microplates show considerably higher transparency compared to other plates made from polypropylene (see Application Note no. 202 ), the Eppendorf Microplates may be used for absorption measurements in certain cases.

Why are the Eppendorf PP Microplates offered in Sterile and PCR clean, but not in Eppendorf quality?

The Eppendorf PP Microplates are a very high quality product. In order to underline the quality, all plates are available in the degrees of purity PCR clean or Sterile. This increases the confidence and comfort of the user, who may employ the plates directly for valuable samples in sophisticated applications.

Are Eppendorf PP Microplates also available with barcode?

Yes, the option of Eppendorf Microplates with barcode is available. Further information can be found on the product page microplates .

Are Eppendorf tubes, plates (also LoBind variants), and pipette tips resistant to 100% acetonitrile?

Eppendorf tubes, plates including LoBind, and pipette tips are resistant to 100% acetonitrile only in room temperature conditions. Above 40°C and prolonged exposure (days) may lead to partial damage and is not recommended. For lower acetonitrile concentrations the resistance parameters will be better so prolonged exposure and higher temperatures may be possible. This must be however tested by the customer.

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Eppendorf Microplates & Assay/Reader Plates

4つのマイクロプレート、384ウェルブラックおよび透明プレート、96ウェルホワイトと透明プレート

Stacked assay reader plates - clear, white and black

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製品情報

卓越した透明性、感度、再現性をMicroplates と Assay/Reader Platesを使用してあなたのマイクロプレートアッセイで達成してください。弊社のプレートは、高品質のポロプロピレンから作られ、最適なウェルパフォーマンスのために設計されており、あなたのワークフローを容易にし、サンプルを効率的に回復させます。強いS/N比と低い検出限界のメリット—毎回あなたの結果を信頼することができます。蛍光や化学発光、それとも可視検知で作業するにしても、あなたのアッセイをサポートする適正なプレートを見つけることができるでしょう。

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製品情報

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特徴

サンプルのピペッティング、コントローリング、リカバリングが弊社のMicroplatesを使用すればシンプルになります。英数字OptiTrack^® マトリックスラベルを簡単に読み取ることは、個々のウェルを迅速に識別することに役立ち、ワークフローを合理化し、ピペッティングエラーのリスクを軽減します。高い透明性のポリプロピレン製の弊社のMicroplates によって、一目でサンプルを簡単に見つけることができます。最大サンプルリカバリーのためには、LoBind^®オプションを選びます。弊社のAssay/Reader platesのポートフォリオは、信頼できる測定のために設計されています—可視領域の吸光、蛍光、または化学発光で作業するにも、全てトップリーディングプレートリーダーのために最適化されています。低濃度サンプルでもクリア信号を体験してみてください。
蛍光検知のためには黒色マイクロプレートを選んでください、強いS/N比が低検知制限をサポートします。発光または弱い蛍光の場合には、弊社の白色のmicroplates が反射を最大化し、高い感度の結果をもたらします。

優れた透明性とOptiTrack^®マトリックス

弊社のEppendorf Microplatesは最高のクオリティのポリプロピレンで製造されており、最大の透明性を保証し、再現性を改善するので安心です。コントラスト豊富なOptiTrackマトリックスで実験室の効率を上げましょう – 記録的なタイムでウェルを識別し、エラーと汚染のリスクを軽減します。
Eppendorf Microplatesが素早くサンプルを識別する方法をもっと詳しく。Application Note 202を読んでください：Eppendorfポリプロピレンマイクロプレート – サンプルを迅速かつ確実に識別できます。

RecoverMax^®ウェル設計を使用して最小の時間で最大の回収

Eppendorf マイクロプレートのRecoverMax^®最適化されたウェルゲオメトリで最大のサンプル回収と優れたミキシング特性を掴んでください。ウェルコーナーの丸型でスムーズな設計がサンプル損失を防ぐ一方、残余量を最小化し、四角いウェルプレートのスムーズな輪郭は交差汚染のリスクを事実上根絶します。

Eppendorf Microplatesは究極的な透明性とクオリティをさまざまなフォーマットで提供します：

• 96および384ウェルフォーマット
• Fボトム（平型）、Uボトム（丸型）、Vボトム（円錐型）。
• Eppendorf PCR clean そしてSterile 純度等級。
• DNAおよび Protein LoBindとしても揃っています。
• g–Safe^®：最大6,000 x という優れた遠心分離の安定性により、高速なプロトコルおよびサンプル品質の改善を実現。
• 縁が高くなっており、表面が滑らかなので信頼できるクロージング、ヒートシーリングも付いています
• 耐薬品性、機械的耐久性、温度安定性が高いポリプロピレン製です。
• オートクレーブ可能（121 °C、20 分）。
• スリップ剤、可塑剤、殺生物剤無しで製造

詳細はApplication Note 56参照：Eppendorf純正チューブ^®、チップ、プレート用に最高級の素材：ポリプロピレンの性能と化学薬品耐性そしてApplication Note 239： Eppendorf Deepwell Platesとマイクロプレート - シーリング方法の投資

Are Eppendorf microplates suitable for fluorescence readers, spectrophotometers, and other microplate readers?

Yes, our microplates are suitable for fluorescence readers, spectrophotometers, and other microplate readers. Whether you’re conducting 96-well plate fluorescence reader assays, 96-well plate spectrophotometer assays, or 384-well plate reader assays, our products provide excellent optical characteristics and low background interference.
However, it’s important that you choose the right plate colour for your intended application. Black microplates for fluorescence help to minimize well-to-well crosstalk and minimize signal-to-noise ratios, making them an excellent choice for sensitive fluorescence applications. White assay plates enhance signal reflection for chemiluminescence assays, while transparent assay plates are well suited for absorbance measurements and routine microplate assays.