For which applications may I use Eppendorf Conical Tubes ® 25 mL?

In general, all applications where standard conical tubes 15mL and 50 mL are routinely used. The Eppendorf Conical Tubes ® 25 mL close a gap between 15 mL and 50 mL conical tubes and additionally offer in this format a unique snap cap – the so called SnapTec™ cap - which markedly improves handling. This makes the Eppendorf Conical Tubes ® 25 mL ideally suited for all cell/tissue culture applications including splitting cells, media change, transfections, etc. Also assay preparation and biomolecule purification protocols (gDNA, plasmid DNA, RNA, proteins), which require organic solvent extractions and multiple centrifugation steps may be successfully performed. They are also ideally suited for general reagent / sample handling and space-saving storage.

Is it necessary to buy new equipment to enable the use of Eppendorf Conical Tubes 25 mL within existing lab periphery?

No. The bottom shape / diameter of the Eppendorf Conical Tubes 25 mL is virtually the same with standard conical 50 mL tubes. With the respective height adapter (provided as Eppendorf accessory), the Eppendorf Conical Tubes 25 mL will be compatible with a wide range of standard laboratory equipment: centrifuges, mixers, etc., additionally tube racks, storage boxes and single-tube stands (skirts) are available for lab bench work, weighing and storage. For detailed accessories description please refer to www.Eppendorf.com .

Is it possible to store samples in 25 mL Eppendorf Conical Tubes in the freezer?

Yes, the tubes can be stored down to -86 °C.

How resistant are the Eppendorf Conical Tubes ® 25 mL to chemicals?

The tubes are made of high quality, transparent polypropylene and resistant to a majority of commonly used chemical substances in the laboratory. An overview is provided in Application Note No. 56. In case a substance/chemical is not listed there, please contact our local contact.

Which purity grades and LoBind / Amber variants are available for Eppendorf Conical Tubes 25 mL?

The Eppendorf Conical Tubes 25 mL are available in the purity grades: Eppendorf Quality, PCR clean and Sterile/Pyrogen-free. For general and lot-specific certificates please refer to Eppendorf website eppendorf.com/certificates. The DNA and Protein LoBind as well as Amber variants of Eppendorf Conical Tubes ® 25 mL will be available within short term as well.

Correct opening and closing of the snap Lid (SnapTec ® ).

The snap cap of the Eppendorf Conical Tubes ® 25 mL is much larger than in other tube formats and may require some short learning of correct closing/opening at the beginning. Opening: press the safety tab from below and steady press upwards. In case of pressure buildup (organic solvents, higher temp incubations) make sure you slightly hold the lid with your thumb and don’t let the lid to pop out rapidly. Closing: press down the lid in the front area and make sure the safety tab clicks and is locked at right angle to the tube. Improperly locked tab may cause leaking.

Are Eppendorf tubes and plates (also LoBind variants) resistant to 100% acetonitrile?

Eppendorf tubes and plates including LoBind variants are resistant against 100% acetonitrile only in room temperature conditions. Above 40°C and prolonged exposure (days) may lead to partial damage and is not recommended. For lower acetonitrile concentrations the resistance parameters will be better so prolonged exposure and higher temperatures may be possible. This must be however tested by the customer.

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Eppendorf Conical Tubes 25 mL

Conical Tube 25mL single-handed operation

Tube Rack 5, 15, 25, 50 mL top view, with medium

Eppendorf Freezer storage box family for 6 different tube volume classes

ACT label of sustainability certification for Eppendorf 25 mL conical tubes (SnapTec, sterile)

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次のレベル – Eppendorf Conical Tubes 25 mL！
大きいEppendorfTube Familyの最新のメンバーは15 mLと50 mLの伝統的な円錐形のチューブの量の間のギャップを埋めます。最適化された高さ、卓越した品質、仕様により、これらのコニカルチューブは細胞生物学、例えばT75フラスコからの培地のプール、ミディプレップサイズでの細菌培養、緩衝液調製、保存、輸送などの重なり合う用途に最適です。

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Eppendorf Conical Tubes 25 mL

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25 mL conical SnapTec sterile (0030118421) (2025) ACT label

How It Works

Eppendorf LoBind® - How it works

Reduced risk of contamination

Improved sample accessibility and recovery

Single-handed operation

製品情報

SnapTec 25mL

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