Are Eppendorf consumables free of RNA?

All consumables certified with "PCR clean" are tested free of human DNA, DNase, RNase and PCR inhibitors. The following applies from our point of view: where no human DNA is detected, there can also be no RNA, which is considerably more unstable.

Are Eppendorf tubes, plates (also LoBind variants), and pipette tips resistant to 100% acetonitrile?

Eppendorf tubes, plates including LoBind, and pipette tips are resistant to 100% acetonitrile only in room temperature conditions. Above 40°C and prolonged exposure (days) may lead to partial damage and is not recommended. For lower acetonitrile concentrations the resistance parameters will be better so prolonged exposure and higher temperatures may be possible. This must be however tested by the customer.

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PCR films & foils

MasterclearリアルタイムPCRフィルムで密封されたA 96ウェルプレート

製品情報

すべてのPCRプレートで信頼性の高いシーリングを実現します。EppendorfPCRフィルムおよびフォイルにより、PCRプレートのシーリングが気密性を確保します。アッセイに適したシーリング材を選択してください。例えば、優れた光学特性を持つMasterclear^®リアルタイムPCRフィルムは、 qPCR に最適です。

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製品情報

アプリケーション

PCRフィルムとフォイルは効果的で気密なプレートシールです。
EppendorfPCRフィルムおよびフォイルは、プレートに効果的で気密性の高いシーリングを提供し、サーマルサイクリングや保存時にPCR試料を保護します。PCRプレートシールは優れた耐薬品性を備えており、さまざまな一般的なラボ用物質に対する耐性がテストされています。最適な光学特性を持つ特殊シールを使用して、qPCR の信号損失を最小限に抑えることができます。

詳細については、 UserGuide 043 （ PCR プレート、ディープウェルプレート、マイクロプレートのシーリングシステムの化学的抵抗の調査）を参照してください。

選択に困っていますか？Eppendorfはマイクロウェルプレート用のさまざまなシーリングシステムを取り揃えております。たとえば、次のようなものがあります：

• 接着フィルムまたはフォイル
•シーリングマット
•プレートリッド
•ヒートシールフィルムまたはフォイル

Adhesive films or foils

• Adhesive films or foils are glued the plate surface.
• Simple handling; however, diligent and equal pressure are necessary to ensure good protection from evaporation.
• Possible influence of the adhesive on the sample has to be taken into consideration.
• Optimal adhesion only at PCR temperatures and should not be used for storage applications.

Sealing mats

• Reusable mats made from an elastomer.
• The plate can be opened and re-sealed at any time.
• The mat needs to be compatible with the plate (round/square wells).

Plate lids

• Reusable transparent lid made from polystyrene.
• Simplest and fastest closure option.
• Mainly used as a temporary cover and for short incubations, since protection from evaporation is limited compared to other sealing options.

PCR Film and Foil (self-adhesive)Heat-sealing foils and films (requires a plate sealer)

• Heat-sealed foils and films are welded to the plates using a heat-sealing device.
• Very secure and effective heat seal.
• Only suitable for plates made from polypropylene or similar materials (including Eppendorf twin.tec^® PCR Plates)
• A raised rim around the individual wells is required for heat seals.

Which sealing system should I choose for my PCR plates?

PCR sealing film or foil?

The right sealing option will highly depend on your intended application. The first choice you should make is whether foils or films are most suitable. In general, foils are well-suited for light-sensitive samples. Foils can also be easily pierced, meaning your samples can be transferred via a needle or automated system. On the other hand, films are a more practical option if you need transparency for optical measurements (e.g. qPCR) and protection from unintended piercing.

Permanent or temporary sealing?

Another key consideration is whether your seal needs to be permanent or temporary. If you need permanent sealing, heat-sealing foils and films are a durable long-term option, providing strong protection against evaporation. For applications requiring temporary sealing, self-adhesive options and sealing mats are an ideal choice, offering easy, residue-free removal.

Conducting qPCR?

Masterclear real-time PCR film is optimized for maximum light transmission. Transmission values of > 90 % between 350 nm and 750 nm guarantee optimal excitation of your fluorescent dyes and optimal readout of the emitted fluorescence.

Using automated systems?

Our Heat Sealing Foils are made of aluminum. They provide a strong heat seal that prevents evaporation, even with low sample volumes. They also can be easily pierced by autosamplers, making them particularly well-suited for high-throughput automated assays.

Sealing option selection guide

Still stuck on which sealing option to choose? Explore the table below for more information to help you choose the best sealing option for your application.

^{Table 1: Eppendorf sealing option selection guide, outlining the main features of each product. The guide includes self-adhesive films and foils, heat-seal films and foils, storage films and foils, sealing mats, plate lids, and options for real-time PCR.}