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GMP info

Regarding the suitability of Eppendorf bioprocess equipment in GMP-regulated applications, please reach out to your Eppendorf sales representative.

Cell Culture in Bioreactors

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Bioreactors can be used for the large-scale cultivation of various cell types, including mammalian cell lines, stem cells , and insect cells. Cell culture in bioreactors has a wide range of applications, including cell and gene therapy development , and the production of antibodies , vaccines , and viral vectors . There are many factors to consider when setting up these bioprocesses, including the optimization of critical process parameters, finding the right feeding strategy, and ensuring bioprocess scalability.

What factors should be considered when establishing bioreactor cell cultures?

The cultivation of human and animal cells in stirred-tank bioreactors offers many benefits: Cell culture in stirred-tank bioreactors is scalable, from research scale in the milliliter range to thousands of liters. High yields can be achieved, and batch-to-batch variability can be kept low. However, to achieve this, optimal growth conditions must be created in the bioreactor. In the following, we look at which factors are particularly important.

The shear force on the cell culture in bioreactors‌

Regulation of critical process parameters‌

Feeding bioreactor cell cultures‌

Prevent contamination of cell cultures in bioreactors‌

Bioprocess scalability‌

Frequently used cell types tailored towards your bioprocessing application of choice

There are several frequently used cell lines in bioreactors, optimized for various applications and bioreactor conditions. Whether it’s animal or human cell lines, stem cells or other primary cells, the cell type most suitable for your application and bioprocess should be carefully chosen. Find more information about the cultivation of different cell types in bioreactors.

Chinese hamster ovary (CHO) cell culture in bioreactors

CHO cells are the workhorses of the biopharmaceutical industry, and are largely used for the production of recombinant proteins such as monoclonal antibodies. Download selected application examples of CHO cell culture in Eppendorf bioreactor systems:

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Stem cell culture in bioreactors

Stem cells are emerging as powerful therapeutic and drug discovery tools. As the stem cells themselves are the final product, parameter control is particularly important to ensure high cell quality and reproducibility. Commonly used stem cells in bioreactors include mesenchymal stem cells and pluripotent stem cells.

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T cell culture in bioreactors

T cell lymphocytes have shown promising results in cell and gene therapies, particularly in the targeting of tumor cells.

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HEK293 cell culture in bioreactors

Derived from human embryonic kidneys, HEK293 cells are frequently used for viral vector production. These cells were initially anchorage-dependent. However, suspension-adapted HEK293 cell lines have been developed to allow for improved productivity and scalability. Download application notes on the use of Eppendorf bioreactor systems for HEK923 cell cultivation:

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Vero cell culture in bioreactors

Vero cells are a widely used cell type for viral vector and vaccine production, and are a type of mammalian cell derived from the kidney epithelial tissue of African green monkeys. However, Vero cell lines are anchorage-dependent, requiring growth in packed-bed bioreactors or microcarrier-based cell culture systems.

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Sf9 cell culture in bioreactors

These are obtained from the ‘fall armyworm’ insect, Spodoptera frugiperda. Sf9 cells are commonly used with baculovirus expression system for the production of viral vectors, vaccines, and proteins. These cells are suspension-adapted to improve handling, productivity, and scalability.

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