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- Challenges and Chances: A Review of the 1st Stem Cell Community Day
- Summertime, and the Livin’ Is Easy…
- Follow-on-Biologics – More than Simple Generics
- Bacteria Versus Body Cells: A 1:1 Tie
- Behind the Crime Scene: How Biological Traces Can Help to Convict Offenders
- Every 3 Seconds Someone in the World Is Affected by Alzheimer's
- HIV – It’s Still Not Under Control…
- How Many Will Be Convicted This Time?
- Malaria – the Battle is Not Lost
- Physicians on Standby: The Annual Flu Season Can Be Serious
- At the Forefront in Fighting Cancer
- Molecular Motors: Think Small and yet Smaller Again…
- Liquid Biopsy: Novel Methods May Ease Cancer Detection and Therapy
- They Are Invisible, Sneaky and Disgusting – But Today It’s Their Special Day!
- How Many Cells Are in Your Body? Probably More Than You Think!
- What You Need to Know about Antibiotic Resistance – Findings, Facts and Good Intentions
- Why Do Old Men Have Big Ears?
- The Condemned Live Longer: A Potential Paradigm Shift in Genetics
- From Research to Commerce
- Chronobiology – How the Cold Seasons Influence Our Biorhythms
- Taskforce Microbots: Targeted Treatment from Inside the Body
- Eyes on Cancer Therapy
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JP | JPY
Automated Liquid Handling Systems Facilitate Small Volume Pipetting
Lab Academy
Automated liquid handling systems have many advantages when handling problematic liquids like viscous or volatile liquids, as well as very small volumes. The systems have strategies to deliver accurate and reliable results with some tricks programmable in the software.
At first, an automated liquid handling system might seem complicated and overwhelming. But once you have started working with these devices, you will realize how they simplify your workflow. Engineers have developed many different features to facilitate challenging applications.
When handling small volumes with automated liquid handling systems, it is possible to aspirate all reagents needed for the reaction in one tip, separated by an air-gap. This technique is widely discussed, especially in terms of contamination of the different liquids by drops on the outside of the pipette tip. Some manufacturers recommend this anyway to save time and effort1. The systems can aspirate the water first, followed by reagent A, then reagent B, etc. Each liquid layer is separated with an air gap to prevent mixing or the reaction starting inside the tip. When the liquid is dispensed, all reagents are mixed directly and the smallest volumes are washed out of the tip by the larger volumes in the tip. The tip should be changed after every pipetting step.
A better option is to use special tools optimized for small volumes, e.g., for transferring volumes of 1 µL in free-jet dispensing. This increases speed and avoids cross-contamination. If volumes below 1 µl are pipetted, it is better to dispense directly into a target liquid or against the vessel surface to dispense the whole volume. Dispensing small volumes with liquid contact is also recommended when challenging liquids such as viscous liquids are pipetted.
Another very helpful feature of automated liquid handling systems is tip dipping. When only 1 µL sample is aspirated into the tip, the liquid drop often sticks to the outside of the tip during dispensing. It is possible to program the tip to dip into the liquid in the well so that drops and micro-drops on the outside surface of the tip reach the reaction.
Furthermore, setting the aspiration and dispensing speed as well as the blow-out volume and speed helps too. The perfect speed for each type of liquid and volume can be programmed. And setting these parameters leads to highly reproducible results because we pipette at different speed every day depending on our personal performance. Automated liquid handling can ease your mind and increase trust in challenging applications by taking over the annoying parts.
1 Astle T W, Small Volume Pipetting, technical report. 1998. Journal of the Association for Laboratory Automation. Vol. 3 No 3, July 1998
When handling small volumes with automated liquid handling systems, it is possible to aspirate all reagents needed for the reaction in one tip, separated by an air-gap. This technique is widely discussed, especially in terms of contamination of the different liquids by drops on the outside of the pipette tip. Some manufacturers recommend this anyway to save time and effort1. The systems can aspirate the water first, followed by reagent A, then reagent B, etc. Each liquid layer is separated with an air gap to prevent mixing or the reaction starting inside the tip. When the liquid is dispensed, all reagents are mixed directly and the smallest volumes are washed out of the tip by the larger volumes in the tip. The tip should be changed after every pipetting step.
A better option is to use special tools optimized for small volumes, e.g., for transferring volumes of 1 µL in free-jet dispensing. This increases speed and avoids cross-contamination. If volumes below 1 µl are pipetted, it is better to dispense directly into a target liquid or against the vessel surface to dispense the whole volume. Dispensing small volumes with liquid contact is also recommended when challenging liquids such as viscous liquids are pipetted.
Another very helpful feature of automated liquid handling systems is tip dipping. When only 1 µL sample is aspirated into the tip, the liquid drop often sticks to the outside of the tip during dispensing. It is possible to program the tip to dip into the liquid in the well so that drops and micro-drops on the outside surface of the tip reach the reaction.
Furthermore, setting the aspiration and dispensing speed as well as the blow-out volume and speed helps too. The perfect speed for each type of liquid and volume can be programmed. And setting these parameters leads to highly reproducible results because we pipette at different speed every day depending on our personal performance. Automated liquid handling can ease your mind and increase trust in challenging applications by taking over the annoying parts.
1 Astle T W, Small Volume Pipetting, technical report. 1998. Journal of the Association for Laboratory Automation. Vol. 3 No 3, July 1998
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