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- Challenges and Chances: A Review of the 1st Stem Cell Community Day
- Summertime, and the Livin’ Is Easy…
- Follow-on-Biologics – More than Simple Generics
- Bacteria Versus Body Cells: A 1:1 Tie
- Behind the Crime Scene: How Biological Traces Can Help to Convict Offenders
- Every 3 Seconds Someone in the World Is Affected by Alzheimer's
- HIV – It’s Still Not Under Control…
- How Many Will Be Convicted This Time?
- Malaria – the Battle is Not Lost
- Physicians on Standby: The Annual Flu Season Can Be Serious
- At the Forefront in Fighting Cancer
- Molecular Motors: Think Small and yet Smaller Again…
- Liquid Biopsy: Novel Methods May Ease Cancer Detection and Therapy
- They Are Invisible, Sneaky and Disgusting – But Today It’s Their Special Day!
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- From Research to Commerce
- Chronobiology – How the Cold Seasons Influence Our Biorhythms
- Taskforce Microbots: Targeted Treatment from Inside the Body
- Eyes on Cancer Therapy
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- Challenges and Chances: A Review of the 1st Stem Cell Community Day
- Summertime, and the Livin’ Is Easy…
- Follow-on-Biologics – More than Simple Generics
- Bacteria Versus Body Cells: A 1:1 Tie
- Behind the Crime Scene: How Biological Traces Can Help to Convict Offenders
- Every 3 Seconds Someone in the World Is Affected by Alzheimer's
- HIV – It’s Still Not Under Control…
- How Many Will Be Convicted This Time?
- Malaria – the Battle is Not Lost
- Physicians on Standby: The Annual Flu Season Can Be Serious
- At the Forefront in Fighting Cancer
- Molecular Motors: Think Small and yet Smaller Again…
- Liquid Biopsy: Novel Methods May Ease Cancer Detection and Therapy
- They Are Invisible, Sneaky and Disgusting – But Today It’s Their Special Day!
- How Many Cells Are in Your Body? Probably More Than You Think!
- What You Need to Know about Antibiotic Resistance – Findings, Facts and Good Intentions
- Why Do Old Men Have Big Ears?
- The Condemned Live Longer: A Potential Paradigm Shift in Genetics
- From Research to Commerce
- Chronobiology – How the Cold Seasons Influence Our Biorhythms
- Taskforce Microbots: Targeted Treatment from Inside the Body
- Eyes on Cancer Therapy
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JP | JPY
384-Well Pipetting - Time to Fire the Rocket
Dr. Stefanie Rösel Lab Academy
384-well microplates incredibly improve our high-throughput lab routines. But for all its advantages, researchers who are dependent on a manual system suffer twice: tiny reaction vessels in an incredible number of 384, waiting for 384 individual samples. Here we show you how you can save 70 % of your valuable time.
Pipetting the smallest volumes by hand into a 384-well format is laborious. The plate is miniaturized, the wells are tiny. Although 8- or 12-channel pipettes make our lives easier when loading buffer, medium, or master mix, there are some tricky circumstances to master. Tip shape and tip distance are optimized for a 96-well system. As they are quite thick compared to the wells, hitting entire rows or columns simultaneously requires the highest concentration. Besides, you can only hit and fill every second well. In the case of the smallest mistake, the complete plate and its valuable contents must be disposed of.
The manual transfer of 384 individual samples then becomes the ultimate bottleneck. Laborious sample maneuvering with single-channel pipettes out of different tubes or plate formats into 384-well plates means pipetting back and forth up to 384 times. For your eyes this is maximum performance and for your joints and muscles ergonomically very demanding.
The manual transfer of 384 individual samples then becomes the ultimate bottleneck. Laborious sample maneuvering with single-channel pipettes out of different tubes or plate formats into 384-well plates means pipetting back and forth up to 384 times. For your eyes this is maximum performance and for your joints and muscles ergonomically very demanding.
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The accelerators
Use pipetting systems that are optimized for 384-well plates . This includes a considerable number of channels, tips specifically shaped to fit the small wells, and an appropriate 4.5 mm tip space adapted to a 384-well plate. The following concept also enables you to set up or validate an automated process manually, and to have a back-up system in case automation fails.
You can benefit from about 50% time-savings by using pipettes with 16/24 channels instead of 8/12 channels! With 16/24-channel pipettes, you can even manually fill a 384-well plate within one minute. Entire columns or rows can be easily equipped, and you don’t need to pipette alternately. Specifically shaped tips ensure to hit the wells ideally. You can concentrate less and make fewer mistakes. Ideally, use a 24-channel pipette for loading master-mix, buffer, or medium.
- 1. 16/24-channel pipettes for master-mix, buffer or medium:
50 % time-saving
50 % time-saving
You can benefit from about 50% time-savings by using pipettes with 16/24 channels instead of 8/12 channels! With 16/24-channel pipettes, you can even manually fill a 384-well plate within one minute. Entire columns or rows can be easily equipped, and you don’t need to pipette alternately. Specifically shaped tips ensure to hit the wells ideally. You can concentrate less and make fewer mistakes. Ideally, use a 24-channel pipette for loading master-mix, buffer, or medium.
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Tipp: With electronic pipettes, the multichannel pipetting of reaction liquids into 384-well plates can be even further accelerated . Using the dispensing mode not only relaxes your muscles and eyes but saves you up to 80 % of the time compared to 8/12-channel pipettes.
Adjustable tip spacing pipettes move multiple samples simultaneously from one vessel format to another. Instead of pipetting 384 times using a single-channel pipette, you can transfer up to 12 samples simultaneously into a 384-well plate.
So, put aside your single-channel pipette, move samples from 96-well plates with a 12-channel adjustable tip spacing pipette, and save up to 70% time! Likewise, for moving samples from tubes, you save up to 64% time by using an 8-channel adjustable tip spacing instead of a single-channel pipette.
- 2. Adjustable tip spacing pipettes for individual samples:
70 % time-saving
70 % time-saving
Adjustable tip spacing pipettes move multiple samples simultaneously from one vessel format to another. Instead of pipetting 384 times using a single-channel pipette, you can transfer up to 12 samples simultaneously into a 384-well plate.
So, put aside your single-channel pipette, move samples from 96-well plates with a 12-channel adjustable tip spacing pipette, and save up to 70% time! Likewise, for moving samples from tubes, you save up to 64% time by using an 8-channel adjustable tip spacing instead of a single-channel pipette.
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- 3. Combine both for maximum outcome
The most efficient way to manually fill a 384-well plate is the combination of both:
- 1. Loading reaction liquid: 24-channel electronic pipette
- 2. Individual sample transfer: Adjustable tip spacing pipette
Both combined save you up to 70% time.
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