What is the best way to dispense organic solvent?

It is best to use a positive displacement device like the Multipette. Positive displacement devices are most suitable for precise and drip-free dispensing of liquids with high vapor pressure. As the liquid is enclosed in a tip with integrated piston, the solvent vapor does not come into direct contact with the instrument.

How can I adjust my air-cushion pipette for challenging liquids, long tips, or higher/lower altitudes?

Upon manufacturing, pipettes are calibrated to distilled water. Eppendorf mechanical pipettes allow temporary user adjustments, beneficial for liquids with different physical properties than water (density, viscosity, surface tension, vapor pressure), or when capillary actions occur (e.g., with DMSO). Adjustments are also beneficial when atmospheric pressure changes due to altitude or when using non-standard pipette tips (e.g., epT.I.P.S. long). The adjusted pipette delivers the correct volume only for the liquid used during adjustment. Please be aware that variable mechanical pipettes turn into "fixed-volume pipettes" when being adjusted. That means they can only be adjusted to a specific liquid to one volume and not across the entire dispensing range. Research plus and Reference 2 pipettes feature an adjustment window where the change can be monitored. It can be set back to factory settings "0". For detailed procedures and examples, refer to the operating instructions and Application Note No. 198. In contrast to mechanical pipettes, the electronic Xplorer/Xplorer plus pipettes can be adjusted for the entire range of volume. Additionally, the electronic pipettes offer several options for adjusting the pipette in the "Options" menu, e.g. to 75 % ethanol, altitude, elongated tips. Furthermore, individual adjustments are possible, as 1-point, 2-point or 3-point settings. For more information see User Guide 036. Temporary user adjustments can be reset to manufacturer settings without recalibration.

How do I determine the volume values when calibrating gravimetrically?

The most common method involves measuring the mass of distilled water (whose physical properties are known) using a balance, and then converting the mass to a volumetric value. This means that the measurement values from the analytical balance are multiplied by the correction factor Z (see SOP pipettes). This adjustment accounts for the density of water at the specific temperature and the relevant air conditions.

What does EN ISO 8655 cover?

ISO 8655-2022 is the global standard for piston-operated volumetric apparatus (POVA), including pipettes, burettes, dilutors, dispensers, and manual precision syringes, as defined by the International Organization for Standardization (ISO). It ensures standardized performance for manufacturers, calibration providers, and users.It is structured in 10 chapters: ISO 8655-1: Terminology, general requirements and user recommendations ISO 8655-2: Pipettes ISO 8655-3: Burettes ISO 8655-4: Dilutors ISO 8655-5: Dispensers ISO 8655-6: Gravimetric reference measurement procedure ISO 8655-7: Alternative measurement procedures ISO 8655-8: Photometric reference measurement procedure ISO 8655-9: Syringes ISO 8655-10: User guidance, user qualification and POVA qualification.

What do the terms random error and systematic error mean in a pipette calibration?

In pipette calibration, random error and systematic error are two key concepts that help ensure accuracy and precision in measurements. Random Error refers to the variability in measurements when the same volume is dispensed multiple times. It is also known as imprecision and is determined by calculating the repeat standard deviation of 10 measurements. These errors tend to scatter around the true value and can be minimized by consistent technique and proper training. Systematic Error is the consistent deviation of the measured volume from the selected/true volume, also known as inaccuracy and is determined by establishing the mean value of 10 measurements. Systematic errors can arise from defective or misadjusted devices and need to be corrected through proper calibration and maintenance.

Why are the Z factors listed in our SOP different from factors which can be derived from density reference values (of water)?

The Z factor is included in EN ISO 8655-6 and was incorporated into our SOP. As we understand it, it was incorporated in the standard by the Physikalisch Technische Bundesanstalt in Braunschweig. The Z factor describes the density of water at a particular temperature using a 5th degree polynomial. Furthermore, the density of water is corrected by the density of air (derived from air pressure) and a fixed correction value for balances. These corrections are the key reasons for the deviation from density values in various books of tables.

At which volumes should a variable pipette be calibrated?

According to the EN ISO 8655-2022 standard, three series of 10 measurements are used to calibrate a variable-volume pipette. The three test volumes are: 1. Nominal volume (the largest volume adjustable by the user, as determined by the manufacturer) 2. 50% of the nominal volume 3. 10% of the nominal volume, or the lower limit if the minimum is over 10%

What is the nominal volume of a variable pipette?

The nominal volume of a variable pipette is always its maximum volume. For a 100 - 1,000 µL pipette, this means 1,000 µL.

I very frequently work with different chemicals. Can I still perform pipette calibration using water?

As per EN ISO 8655-2022, pipette calibration, which involves checking liquid dispensed by the pipette on a balance, should always be performed using distilled or deionized water. If you are working with chemicals that have different properties than water, consider using the temporary adjustment to minimize errors as outlined in Application Note 198. In general, we recommend using direct displacement devices such as the Multipette M4/E3(x) for challenging liquids.

During weighing (pipette calibration) I obtained steadily decreasing measuring results. Why is this?

The weight fluctuations you measured would appear to be a temperature problem. Please ensure that the pipette, the distilled water used for testing, the tips etc. are all at the same temperature. It is recommended that the pipettes be placed in the weighing room at least 2 hours before the test is performed. While measuring, ensure that transfer of heat from your hands is kept to a minimum (otherwise weight fluctuations may occur!) Tip: do not constantly grasp the pipette in the palm of your hand while waiting for each measurement result! We recommend our standard operating procedure (SOP) for pipettes as a source of basic information on pipette calibration.

How are multi-channel pipettes tested?

According to ISO 8655-2022, every channel of a multi-channel pipette must be tested. While all channels need to be filled, only the channel being tested is measured. Due to the high number of measurements required (e.g., 240 measurements for an 8-channel pipette and 360 measurements for a 12-channel pipette), it is advisable to send the multi-channel pipette to a professional calibration lab. These labs are equipped with specialized balance setups that ensure all channels are filled while only one channel is measured at a time.

Is the pipette grease free of resins?

Yes, the pipette grease supplied by Eppendorf is free of resins.

Is a gravimetric test necessary after resetting the adjustment display to "0"?

From a technical point of view, a gravimetric test is not required. However, for certified laboratories a gravimetric test might be required for documentation purposes.

How many overnight cycles or total hours of UV irradiation can the Research plus withstand before any discoloration or change of the material will be visible?

We tested UV radiation under very intense conditions for 165 hours. There was no visible discoloration or change to the material. We recommend sterilizing Research plus pipettes with UV light 30 W at 254 nm and a distance of 60 cm.

Can the Research plus be autoclaved if the locking ring is installed to deactivate the spring loading?

Yes. The locking ring is made of PPS (polyphenylene sulfide) and therefore autoclavable (121 °C, 1 bar, 20 min).

Do I need to disassemble the Research plus before autoclaving?

No. It can be autoclaved in one piece (121 °C, 1 bar, 20 min).

Will water vapor enter the pipette while it is being autoclaved, causing water vapor to bead up on the inside of the numerical volume setting display?

Vapor will penetrate the inside of the pipette during autoclaving but this will not cause damage. We tested our pipettes with 175 autoclaving cycles (121 °C, 1 bar, 20 min). The number of tests chosen corresponds to an average of 5 years of use.

In part 3 of the operating manual of the Research plus, you recommend adjusting the volume setting from a higher value to a lower value. If required, the user should turn the value beyond the required value and then back again. What is the reason for such a recommendation?

For technical reasons, the results are generally more precise when a volume setting is carried out from a higher down to a lower value. This is valid for all manual pipettes.

How should the Research plus be disinfected?

Wipe the outer surface with disinfectant, DNA/RNA decontamination agent, or 70 % isopropanol or a commercially available disinfectant. Check the chemical resistance of the pipettes using the chemical resistance table (website). After the exposure time of the disinfectant, rinse thoroughly with demineralized water. This step is especially important if subsequent decontamination, e.g., with UV-light or autoclaving, should be performed. The Research plus may be temporarily subjected to UV light under a sterile bench. We recommend the use of a Hg-lamp, 254 nm, 30 W. The distance between lamp and pipette should be approx. 60 cm. The Research plus is fully autoclavable.

How can I clean and maintain the Research plus?

The Research plus pipettes are easy to clean. Only the lower part should be cleaned on a regular basis in accordance with the frequency of use. The lower part of the single-channel and multi-channel Research plus pipettes can be removed easily as described in the operating manual of the Research plus or in our Application Note 198.

Is it possible to inactivate the spring-loaded tip cone of a single-channel pipette?

Yes, the delivery includes a locking ring to easily deactivate the spring-loaded tip cone. Disassemble the upper from the lower part of the pipette and push the locking ring onto the lower part from the top. Refer to the Operating Manual for an illustrated guide. This is useful for pipette tips not designed for Eppendorf pipette cones.

What is the difference between factory setting and user adjustment?

Eppendorf pipettes offer factory adjustment and temporary adjustment. The temporary adjustment is an easy way of adjusting the pipette temporarily to different conditions (e.g. pipetting a liquid with high density). The factory adjustment should be changed if the pipette was found to be out of specifications during calibration. Before adjusting, exclude other causes such as pipette handling, environmental errors, or worn-out parts.

How often should pipettes be calibrated?

The user determines the frequency of calibration. However, according to EN ISO 8655-2022 Part 1, calibration must be performed regularly and at a minimum, once per year. Routine tests should be performed more frequently, ideally at intervals shorter than those for calibration. The frequency of both calibration and routine tests should be established through a risk assessment. This assessment needs to consider factors such as the frequency of pipette use, the number of users, the aggressiveness of the liquids being dispensed, and the acceptable error limits set by the user. Additionally, any industry- or organization-specific frequency requirements must be adhered to.

I have noticed that more solution is used for reverse pipetting than for normal pipetting. What is the reason for this?

Reverse pipetting means uptake of liquid including the blow-out volume and dispensing of liquid without the blow-out volume. Reverse pipetting is recommended to reduce the error with viscous or foaming liquids or when dispensing very small sample volumes. The excess solution aspirated remains in the tip and is discarded.

What is reverse pipetting using an air-cushion pipette? Is there literature available?

Unlike forward pipetting, reverse pipetting involves pressing the pipette button to the second pressure point, allowing a greater volume to be aspirated into the tip. When dispensing liquid, there’s no pipette blow-out; instead, the residual liquid remains in the tip. This technique offers enhanced accuracy and precision for pipetting liquids with high viscosity or those prone to foam or produce bubbles. Eppendorf offers a variety of literature explaining the technique and proving application examples, such as the Userguide 020, several webinars, and YouTube videos.

What is pre-wetting using an air-cushion pipette, and is it volume-independent?

Regardless of the volume, the air cushion should be wetted by multiple filling and emptying of the tip before dispensing.

What are effective techniques for pipetting viscous liquids?

When pipetting viscous liquids, we recommend positive displacement instruments such as the Multipette (M4, E3, E3x). Those instruments offer the best dosing accuracy for viscous liquids due to the piston integrated into the tip. When handling viscous liquids with an air-cushion pipette (Research plus, Reference 2), be sure to pipette slowly and use reverse pipetting (see Userguide 020 or our extensive eBook on www.eppendorf.com/Pipetting-eBook for guidance). Additionally, adjust your pipette settings to account for differences in density by using the temporary adjustment. However, results will not match those achieved with a positive displacement device.

Are your pipettes free of nucleic acids?

We cannot guarantee that our pipettes are completely free of nucleic acids. While we maintain high standards of cleanliness and care during production, we do not implement specific measures to prevent nucleic acid contamination. To protect your samples from potential contamination, we recommend using positive displacement devices or filter tips, such as ep Dualfilter T.I.P.S. or ep Dualfilter T.I.P.S. SealMax.

When should I grease my Eppendorf pipettes?

The piston or cylinder in the lower part of the pipette should be relubricated after cleaning, decontamination, or exposure to grease-dissolving solutions such as organic solvents. Re-greasing your pipette after autoclaving is not necessary.

How should warm or cold liquids be pipetted?

Best Practices for Pipetting Warm or Cold Liquids: Warm and cold liquids can behave differently than those at room temperature, requiring adjustments in pipetting techniques. Temperature differences between the liquid and the pipette can affect the air cushion, resulting in inaccuracies and imprecision that may exceed permissible error limits. To achieve optimal results, ensure that the pipette, tips, liquid, and surrounding air are all at the same temperature. For enhanced accuracy and precision, consider using a positive displacement device like the Multipette M4 or E3/E3x.

Can the heat of my hand affect my pipetting result?

Modern pipettes address this issue by ensuring sufficient spatial separation between the piston and the handle. Additionally, the materials used in our pipettes support thermal shielding/isolation to prevent heat transfer from your hand (see Userguide 021).

Do Eppendorf pipettes always have to be kept in a stand or can they also be put down on the laboratory bench?

The pipette can be placed on the laboratory bench, but never with liquid inside the tip. In general, it is best to keep pipettes on the pipette carousel. When storing pipettes in a drawer, ensure the pipette does not come into contact with the drawer walls or other equipment, as this may damage the pipette when the drawer is opened or closed.

What material is my pipette made of and what chemicals is it resistant to?

Please refer to the Operating Manual on our homepage for information on material or chemical resistance before use. The Research plus and Reference 2 have separate chemical resistance sheets. Please note that aggressive chemicals used over a longer period of time may shorten the lifetime of the pipette.

My Research plus/Xplorer plus pipette with 20 µL has the color code "pearl white". Which tips should I use?

Eppendorf changed the color code of all 20 µL pipettes and epT.I.P.S. ® 384 tips to light pink. You can use light pink tips with your pipette in pearl white. As part of our service offering, we offer to convert 20 µL pipettes sent in to light pink free of charge. This avoids confusion with the appropriate tips.

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Eppendorf Research^® plus

Eppendorf Research® plus難しい液体の二次調整オプション - クローズアップ

Eppendorf Research® plus 8と12チャンネル機械ピペットおよび96ウェルプレート

Eppendorf Research® plus 16 and 24-channel mechanical pipettes and 384-well plate

Eppendorf 16チャンネルと24チャンネルピペットは384ウェルプレートの液体を分注します

アグロ―ゲルへピペッティングするEppendorf Research® plus 24チャンネルピペット

Male laboratory technician works at the bench with PCR, Eppendorf ThermoMixer® and dispenses liquid in a plate

Female Scientist using Eppendorf Research® plus Single-Channel Pipette

ACT label of sustainability certification for Eppendorf Research® plus pipette, 100-1000 µL (blue), single-channel, variable

製品情報

Eppendorf Research plusは、現在入手可能な最も安全で最も人間工学的なメカニカルピペットの1つです。幅広い製品で高い柔軟性を提供しながらも、最も高い精密性と正確性のニーズを満たすResearch plusは、実験室に欠かすことのできないパートナーとなることでしょう。このマイクロピペットは驚異的に軽く、重量とピペッティング力の両方の点で、お客様の手と腕の緊張を回避するのに役立ちます。新しい16と24チャンネルピペットシステムは立証されたコンセプトを新しい技術革新と組み合わせ、384ウェルプレートにおける迅速＆簡単な手動ピペッティングを促進します。

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製品情報

アプリケーション

特徴

労力不要な軽量ピペッティング

このEppendorf Research plusメカニカルピペットは60年を超える経験から生まれたものであり、世界で最も広く使用されているピペットの1つです。先駆的な Eppendorf PhysioCare Concept ^®のおかげで、Research plusは極めて人間工学的に作られており、実験室でのお客様の健康を守ります。非常に軽量で、操作に必要な力が小さいという特長を備えており、また極めて信頼性が高く、完全にオートクレーブ可能で使いやすい製品です。
容量固定または容量可変のシングルチャンネルピペット、8、12、16、24チャンネルから選べるマルチチャンネルピペット、Research plus Move It チップ間隔が調整可能なマイクロピペットなどの幅広い選択肢を取り揃えており、自信を持ってお客様の用途に最適な製品をお届けします。
正しい事、つまり世界でも有数で高度なピペットで作業をしていることを知るのはいい事です。スプリング式チップコーン、一時的調整オプション、改善された容量ディスプレイ、そしてこういったすべてが軽量、フルオートクレーブ可能なピペットに含まれています：これがEppendorf Research plus手動ピペットです。 2021年、可変容量付きのResearch plus シングルチャンネルピペットのラインナップは My Green Lab^®非営利組織によってACT^®Environmental Impact Factor Labelの公認を受けました。これによって、より持続可能製品を探す実験室にとって理想的な選択であることが証明されました。

「Seal of Quality」を受賞

Research plus機械式実験室ピペットは SelectScience^®による「SelectScience Seal of Quality」を受賞しました。これらのシールは、評価の高いSelectScienceプラットフォームで 100 件を超える肯定的なレビューを獲得した、研究室で使用されている製品の上位 0.1 % に付与されます。この賞は科学者に「かけがえのない」洞察を提供したと称賛され、彼らが彼らの職場環境に理想的な実験室製品を選択するのを助けることを意図しています。

どのレベルのフレキシビリティと安全性をお客様は必要としていますか？

最高のフレキシビリティのための可変容量ピペット
• 0.1 µL～10 mLの範囲内の分注容量を調整します
• 選択した容量は大型の4桁容量ディスプレーに表示されます
• 迅速で正確な容量設定
• シングル、8、12、16、24チャンネル並びにMove It チップ間隔調整可能ピペット

迅速で信頼できるワークフローのための固定容量ピペット
• 固定設定容量でより速いペースで作業
• 可変容量のピペットと比べて誤りの可能性を軽減
• 校正が速く簡単
• 12種類のシングルチャンネルピペットが揃っています
• 初心者と限られた予算の実験室向き

アプリケーションのニーズのために適正なピペットを選択してください

すべての実験が同じ作業条件を基にしているわけではありません。容器のタイプによって、チューブに理想的なシングルチャンネルマイクロリッターピペットを選び、プレートにはより良く合うマルチチャンネルピペットを選びます。

• チューブ：固定容量または可変容量シングルチャンネルピペット
• 96ウェルと24ウェルプレート付き： 8および12チャンネルピペット
• 384ウェルプレート： 16および24チャンネルピペット
• 容器の種類変更：Move It 調整可能なチップ間隔ピペット

疲れない人間工学的なピペッティング

Eppendorf Research plusマイクロリッターピペットは直観的に操作でき、完璧なバランスを供給し、オペレーターの緊張と疲労を最小化します。 Eppendorf PhysioCare Conceptが備わったラインで開発されました。結果は軽量でウェルバランスが取れたピペットで、ユニークなスプリング式チップコーンとSoftEject特長が装備されています。操作に必要な力を、Research plus16と24チャンネルピペットをepT.I.P.S.ピペットとテクノロジーを使って組み合わせることによってさらに軽減することができます。 SoftAttach^® テクノロジーを採用した 384 個のチップ。

信頼性の高いチップのフィットおよび低いチップ取り付け力のためのばねバネ式ノーズコーン

バネ式ノーズコーンを備えた Eppendorf ピペットは、エネルギーと時間を節約します。触覚フィードバックにより、軽く 1 回押すだけでチップを簡単に取り付けることができます。スプリングリミットアタッチメントと排出は再現性のある安全なチップのフィットを強制して保証します。この賢い設計により、ユーザー対ユーザーの再現性が向上し、異なる実験室メンバー間でより一貫した結果が得られます。 Eppendorf から 1,000 µL までのすべてのマイクロピペット（Move It 300 µL モデルを除く）には、バネ式ノーズコーン機能が装備されています。

SoftEjectテクノロジーによりチップ排出力が50 %減少

Eppendorfのすべての非調整式マルチチャンネルピペットは、革新的なSoftEjectテクノロジーを使用して、各チップ排出ストローク中に外側のチップが内側のチップより先に排出されるようにします。これはユーザーにはわかりませんが、チップ排出力が 50 % 減少します。イジェクトボタンをわずかに1回押すだけで、自動的にチップが連続して排出されます。

機械式マルチチャンネルピペットでワークフローをスピードアップ

24、96、384ウェルマイクロプレートを簡単かつ迅速に充填する必要がありますか？軽量でEppendorf Research plus8、12、16、24チャンネルを使うと、高容量精密作業をより速く行うことができます。バネ式チップコーンは、揺れを必要とせずに、迅速かつ確実にマルチチャンネルチップをロードするのに役立ちます。すべてのチャンネルで非常に一貫性のある試料収集を行い、ユーザー間の再現性を最大限に高め、実験室のメンバー間でより均一な結果を得ることができます。

96ウェルプレート
96ウェルプレートをコーン距離9 mmのResearch plus8または12チャンネルピペットで簡単に充填できます。弊社の 1,200 µL 容量ピペットは、ディープウェルプレートの充填に最適です。

9 mm間隔のepT.I.P.S.チップを選択する

384ウェルプレート
384ウェルプレートでのアプリケーションには、Research plus16または24チャンネルピペット、または特選Move Itコーン距離 (最小) 4.5 mmの調整可能なチップ間隔を備えた特定のピペットモデルを選択してください。行または列を一杯にして、ワンステップだけで同時に16または24の反応を開始します。

4.5mm間隔のepT.I.P.S. 384チップをお選びください

384ウェルプレートへの迅速で効率的なピペッティング

384ウェルプレートアプリケーション用の効率的な手動ピペッティング方法が必要ですか？ Eppendorfの16チャンネルと24チャンネルピペットは384ウェルプレートとの作業を快適で人間工学的で安全にします。

お客様のメリット
• 8チャンネルまたは12チャンネルピペットを使用する退屈な代替ウェルピペッティングは過去の事です。
• 4つの96ウェルプレートの代わりに一つの384ウェルプレートを処理することは、ピペッティングステップが50 %軽減するのでピペッティングの効率を改善します
• 16または24の反応を同時に同じ開始条件でスタートさせます
• 偶発的なウェルの誤認と実験の繰り返しを回避します
• epT.I.P.S. 384ピペットチップはこれらのマイクロリッターピペットと完全に調和するように設計されています

16チャンネルと24チャンネルピペット用SoftAttach^®テクノロジー付き

epT.I.P.S. 384

初回用に、ピペットチップには伸縮性のあるフォーミンググローブが装備されています。チップコーンの形状があらかじめ定義されているようにチップを伸ばすことができます。その一方で、チップの取り付けおよび排出力は大幅に低減される。 Eppendorf PhysioCare Conceptの最新式を使用することで、このシステムは操作力が最小に軽減する間に完璧なチップシールを可能にします。

お客様のメリット：
• 確実なチップの装着と締め付け
• スム―ズで均等なチップアタッチメント
• チップアタッチメント力が8チャンネルおよび12チャンネルピペットと比べて40 %減少
• 排出力が軽減されます
• 安全なプレート操縦と液体移送のための特別な同軸性による完璧なチップアラインメント
• すべての384ウェルを完璧にヒットするための極めて微細なチップ形状

ライフサイクル終了時点でのパッケージとピペットの廃棄

Eppendorfピペットおよび分注器のパッケージにはさまざまな材質が含まれており、これには段ボール、ビニールフィルム、ピペットチップの箱などがあります。当社のフリーザー包装用段ボールの材料は、約 77 ％リサイクル材料で構成されています。段ボール箱は回収し、お客様の施設で適切な回収容器に入れて廃棄し、貴重な原材料をリサイクルするという世界規模の持続可能性の取り組みにご協力ください。 LD-PE製のラッピングホイルの点で、PE材をリサイクリングできる専門のリサイクリングパートナーを選択することを推奨します。

Eppendorfリキッドハンドリング器機は長年使用することを想定して設計されています – しかし、廃棄の必要性が生じた場合には汚染されている可能性に注意してください。そのため、ピペットや分注器のリサイクルは困難な場合があります。地域のバイオセーフティ担当者や廃棄物管理部にご相談ください。多くの国では、このような廃棄物は法規制があるため焼却する必要があります。実験室用品の廃棄処分に経験のある地域の公認リサイクリングパートナーと協力する他、他の方法があるかを調べることを推奨します。